Microfluidics and fluorescence microscopy protocol to study the response of C. elegans to chemosensory stimuli

Autor: Christine W. Bruggeman, Guus H. Haasnoot, Erwin J.G. Peterman

Publikováno v: STAR Protocols, Vol 4, Iss 1, Pp 102121- (2023)

Summary: Here, we present a protocol to use microfluidics in combination with fluorescence microscopy to expose the C. elegans tail to chemosensory stimuli. We describe steps for the preparation of microfluidic chips and sample preparation through th

Externí odkaz: https://doaj.org/article/e1ccc2a00f464d5984f0079ca1d4d374

Nonlinear mechanics of human mitotic chromosomes

Autor: Anna E. C. Meijering, Kata Sarlós, Christian F. Nielsen, Hannes Witt, Janni Harju, Emma Kerklingh, Guus H. Haasnoot, Anna H. Bizard, Iddo Heller, Chase P. Broedersz, Ying Liu, Erwin J. G. Peterman, Ian D. Hickson, Gijs J. L. Wuite

Publikováno v: Nature
Nature, 605(7910), 545-550. Nature Publishing Group
Meijering, A E C, Sarlós, K, Nielsen, C F, Witt, H, Harju, J, Kerklingh, E, Haasnoot, G H, Bizard, A H, Heller, I, Broedersz, C P, Liu, Y, Peterman, E J G, Hickson, I D & Wuite, G J L 2022, ' Nonlinear mechanics of human mitotic chromosomes ', Nature, pp. 545–550 . https://doi.org/10.1038/s41586-022-04666-5
Meijering, A E C, Sarlós, K, Nielsen, C F, Witt, H, Harju, J, Kerklingh, E, Haasnoot, G H, Bizard, A H, Heller, I, Broedersz, C P, Liu, Y, Peterman, E J G, Hickson, I D & Wuite, G J L 2022, ' Nonlinear mechanics of human mitotic chromosomes ', Nature, vol. 605, no. 7910, pp. 545-550 . https://doi.org/10.1038/s41586-022-04666-5

In preparation for mitotic cell division, the nuclear DNA of human cells is compacted into individualized, X-shaped chromosomes1. This metamorphosis is driven mainly by the combined action of condensins and topoisomerase IIα (TOP2A)2,3, and has been

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8c4d0e3b888b6632e4d6ea88afe55b9

Publisher Correction: Nonlinear mechanics of human mitotic chromosomes

Autor: Anna E. C. Meijering, Kata Sarlós, Christian F. Nielsen, Hannes Witt, Janni Harju, Emma Kerklingh, Guus H. Haasnoot, Anna H. Bizard, Iddo Heller, Chase P. Broedersz, Ying Liu, Erwin J. G. Peterman, Ian D. Hickson, Gijs J. L. Wuite

Publikováno v: Meijering, A E C, Sarlós, K, Nielsen, C F, Witt, H, Harju, J, Kerklingh, E, Haasnoot, G H, Bizard, A H, Heller, I, Broedersz, C P, Liu, Y, Peterman, E J G, Hickson, I D & Wuite, G J L 2022, ' Publisher Correction: Nonlinear mechanics of human mitotic chromosomes ', Nature, vol. 607, no. 7920, E23 . https://doi.org/10.1038/s41586-022-05051-y

In the version of this article initially published, Extended Data Fig. 5 was a duplicate of Extended Data Fig. 6. The correct image is now in place in the HTML and PDF versions of the article.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::623d3b3b5bdaa32949b9f01d665655d1
https://doi.org/10.1038/s41586-022-05051-y