In-vivo Single-Molecule Imaging in Yeast: Applications and Challenges

Autor: Sheetal Paliwal, Nitesh Kumar Podh, Ayan Das, Partha Dey, Shruti Morjaria, Gunjan D. Mehta

Publikováno v: Journal of molecular biology. 433(22)

Single-molecule imaging has gained momentum to quantify the dynamics of biomolecules in live cells, as it provides direct real-time measurements of various cellular activities under their physiological environment. Yeast, a simple and widely used euk

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccda7109c4b27906a23287ff237eef33
https://pubmed.ncbi.nlm.nih.gov/34537238

Functional characterization of kinetochore protein, Ctf19 in meiosis I: an implication of differential impact of Ctf19 on the assembly of mitotic and meiotic kinetochores inSaccharomyces cerevisiae

Autor: Meenakshi Agarwal, Gunjan D. Mehta, Santanu K. Ghosh

Publikováno v: Molecular Microbiology. 91:1179-1199

Summary Meiosis is a specialized cell division process through which chromosome numbers are reduced by half for the generation of gametes. Kinetochore, a multiprotein complex that connects centromeres to microtubules, plays essential role in chromoso

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6a1c586d693ab57957b9ddf8bf663b74
https://doi.org/10.1111/mmi.12527

Single molecule tracking of Ace1p in Saccharomyces cerevisiae defines a characteristic residence time for non-specific interactions of transcription factors with chromatin

Autor: Tatiana S. Karpova, James G. McNally, David A. Ball, Tatsuya Morisaki, Gunjan D. Mehta, Davide Mazza, Florian Mueller, Ronit Salomon-Kent

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2016, 44 (21), pp.e160. ⟨10.1093/nar/gkw744⟩
Europe PubMed Central
Nucleic Acids Research, 2016, 44 (21), pp.e160. ⟨10.1093/nar/gkw744⟩

In vivo single molecule tracking has recently developed into a powerful technique for measuring and understanding the transient interactions of transcription factors (TF) with their chromatin response elements. However, this method still lacks a soli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c352f77bbbd373ef29b324718735056e
https://hal-pasteur.archives-ouvertes.fr/pasteur-01622680/document

Optimization of medium constituents for improved chitinase production byPaenibacillussp. D1 using statistical approach

Autor: Anil Kumar Singh, H. S. Chhatpar, Gunjan D. Mehta

Publikováno v: Letters in Applied Microbiology. 49:708-714

Aims: Statistical optimization of medium components for improved chitinase production by Paenibacillus sp. D1. Methods and Results: Urea, K2HPO4, chitin and yeast extract were identified as significant components influencing chitinase production by P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc033a1d4511f4755c170eff0369b1e7
https://doi.org/10.1111/j.1472-765x.2009.02731.x

Role of Ctf3 and COMA subcomplexes in meiosis: Implication in maintaining Cse4 at the centromere and numeric spindle poles

Autor: Meenakshi Agarwal, Gunjan D. Mehta, Santanu K. Ghosh

Publikováno v: Biochimica et biophysica acta. 1853(3)

During mitosis and meiosis, kinetochore, a conserved multi-protein complex, connects microtubule with the centromere and promotes segregation of the chromosomes. In budding yeast, central kinetochore complex named Ctf19 has been implicated in various

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8b0ffa84baecc73d39b3c117661cf5c
https://pubmed.ncbi.nlm.nih.gov/25562757