Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Gunasekaran Dhandapani"'
Publikováno v:
Nano Select, Vol 4, Iss 6, Pp 386-394 (2023)
Abstract As downstream purification and separation technologies progress towards raising the concentration of therapeutic‐grade monoclonal antibodies (mAbs) in cell culture, downstream processing has begun to face increased difficulty in efficientl
Externí odkaz:
https://doaj.org/article/0c4a1b00aaaa4af0a74506ecddbf28bf
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Abstract The research described in this report seeks to present proof-of-concept for a novel and robust platform for purification of antibody fragments and to define and optimize the controlling parameters. Purification of antigen-binding F(ab′)2 f
Externí odkaz:
https://doaj.org/article/e82c8facc746412893f993be950f9808
Autor:
Sarma, Abhijit1 (AUTHOR), Gunasekaran, Dhandapani1 (AUTHOR), Phukan, Homen1 (AUTHOR), Baby, Akhil2 (AUTHOR), Hariharan, Suneetha1 (AUTHOR), De, Arun Kumar3 (AUTHOR), Bhattacharya, Debasis3 (AUTHOR), Natesan, Sankar2 (AUTHOR), Tennyson, Jebasingh4 (AUTHOR), Madanan, Madathiparambil Gopalakrishnan1 (AUTHOR) madanan.mg@icmr.gov.in
Publikováno v:
Applied Microbiology & Biotechnology. Jul2023, Vol. 107 Issue 13, p4275-4289. 15p.
Industrial scale production of therapeutic monoclonal antibodies (mAbs) is commonly achieved with Protein A chromatography, a process that requires exposure of the antibody to strongly acidic conditions during the eluting step. Exposure to acid inact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6bca79306334df39cc4dc303dece6b7
https://doi.org/10.22541/au.165237513.31436973/v1
https://doi.org/10.22541/au.165237513.31436973/v1
Publikováno v:
Biotechnology and bioengineering. 119(7)
Immunoglobulin M (IgM) antibodies hold promise as anticancer drugs and as agents for promoting immune homeostasis. This promise has not been realized due to low expression levels in mammalian cells producing IgM class antibodies, and the failure of p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d82a364ab8663533ed91898a7b2bce09
https://pubmed.ncbi.nlm.nih.gov/35324016
https://pubmed.ncbi.nlm.nih.gov/35324016
Akademický článek
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Publikováno v:
New biotechnology. 61
We have recently described a non-chromatographic, ligand-free approach for antibody (Ab) purification based on specially designed [Tween-20:bathophenanthroline:Fe2+] aggregates. To assess the potential generality of this approach, a variety of deterg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e771d966ba8fe33d394bce09deb66974
https://pubmed.ncbi.nlm.nih.gov/33279718
https://pubmed.ncbi.nlm.nih.gov/33279718
Autor:
Thoduvayil Sikha, M. Kiran Kumar, Sneha M. Pinto, P.K. Satheeshkumar, T. S. Keshava Prasad, Manish Kumar, Madathiparambil G. Madanan, Jebasingh Tennyson, Krishna Patel, Harsha Gowda, Vikram Kumar, Gunasekaran Dhandapani
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1866:712-721
Leptospira, the causative agent of leptospirosis is known to have many proteases with potential to degrade extracellular matrix. However, a multipronged approach to identify, classify, characterize and elucidate their role has not been attempted. Our
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b4094ad79f700db81b066541d40845e
https://doi.org/10.1016/j.bbapap.2018.04.006
https://doi.org/10.1016/j.bbapap.2018.04.006
Autor:
Gayatri Ramakrishnan, Gunasekaran Dhandapani, Prachi Mehrotra, Narayanaswamy Srinivasan, Madathiparambil G. Madanan
Publikováno v:
Molecular BioSystems. 13:883-891
Leptospirosis, a potentially life-threatening disease, remains the most widespread zoonosis caused by pathogenic species of Leptospira. The pathogenic spirochaete, Leptospira interrogans, is characterized by its ability to permeate human host tissues
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88ca09baeee36e76e6bfd130c48d67eb
https://doi.org/10.1039/c6mb00856a
https://doi.org/10.1039/c6mb00856a
Autor:
T. S. Keshava Prasad, Rahul Brahma, Madathiparambil G. Madanan, Manish Kumar, Sneha M. Pinto, Sikha Thoduvayil, Rex Devasahayam Arokia Balaya, Krishna Patel, Mahesh J. Kulkarni, Jebasingh Tennyson, Gunasekaran Dhandapani, Kiran K. Mangalaparthi, Padikara K. Satheeshkumar
Publikováno v:
PROTEOMICS. 20:2000170
The Triton X-114-based solubilization and temperature-dependent phase separation of proteins is used for subcellular fractionation where, aqueous, detergent, and pellet fractions represents cytoplasmic, outer membrane (OM), and inner membrane protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a969a3f7fd367d7290e4f4d1b8b2655d
https://doi.org/10.1002/pmic.202000170
https://doi.org/10.1002/pmic.202000170