Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Gunar, Fabig"'
Autor:
Georg Kislinger, Gunar Fabig, Antonia Wehn, Lucia Rodriguez, Hanyi Jiang, Cornelia Niemann, Andrey S Klymchenko, Nikolaus Plesnila, Thomas Misgeld, Thomas Müller-Reichert, Igor Khalin, Martina Schifferer
Publikováno v:
eLife, Vol 12 (2024)
Like other volume electron microscopy approaches, automated tape-collecting ultramicrotomy (ATUM) enables imaging of serial sections deposited on thick plastic tapes by scanning electron microscopy (SEM). ATUM is unique in enabling hierarchical imagi
Externí odkaz:
https://doaj.org/article/fdde08b96f784ce684b588b47341e556
Publikováno v:
Bio-Protocol, Vol 13, Iss 20 (2023)
For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic s
Externí odkaz:
https://doaj.org/article/5b39e4cd608b41edb56b197f5e811dba
Autor:
Robert Kiewisz, Gunar Fabig, William Conway, Daniel Baum, Daniel Needleman, Thomas Müller-Reichert
Publikováno v:
eLife, Vol 11 (2022)
During cell division, kinetochore microtubules (KMTs) provide a physical linkage between the chromosomes and the rest of the spindle. KMTs in mammalian cells are organized into bundles, so-called kinetochore-fibers (k-fibers), but the ultrastructure
Externí odkaz:
https://doaj.org/article/91c889fd98c347b5a706552efa28bb7d
Autor:
William Conway, Robert Kiewisz, Gunar Fabig, Colm P Kelleher, Hai-Yin Wu, Maya Anjur-Dietrich, Thomas Müller-Reichert, Daniel J Needleman
Publikováno v:
eLife, Vol 11 (2022)
During eukaryotic cell division, chromosomes are linked to microtubules (MTs) in the spindle by a macromolecular complex called the kinetochore. The bound kinetochore microtubules (KMTs) are crucial to ensuring accurate chromosome segregation. Recent
Externí odkaz:
https://doaj.org/article/20af85c9d44a467fa6ea5249a367b0c4
Autor:
Leocadia V Paliulis, Emily L Stowe, Leila Hashemi, Noemi Pedraza-Aguado, Cynthia Striese, Silke Tulok, Thomas Müller-Reichert, Gunar Fabig
Publikováno v:
PLoS ONE, Vol 17, Iss 8, p e0272978 (2022)
Praying mantids are important models for studying a wide range of chromosome behaviors, yet few species of mantids have been characterized chromosomally. Here we show that the praying mantid Hierodula membranacea has a chromosome number of 2n = 27, a
Externí odkaz:
https://doaj.org/article/e2688808eba44e86a74bdf925aa8be88
Publikováno v:
Bio-Protocol, Vol 10, Iss 20 (2020)
Live-imaging of meiotic cell division has been performed in extracted spermatocytes of a number of species using phase-contrast microscopy. For the nematode Caenorhabditis elegans, removal of spermatocytes from gonads has damaging effects, as most of
Externí odkaz:
https://doaj.org/article/91ca4e30cb734f1a97e61545b32d476b
Autor:
Reza Farhadifar, Che-Hang Yu, Gunar Fabig, Hai-Yin Wu, David B Stein, Matthew Rockman, Thomas Müller-Reichert, Michael J Shelley, Daniel J Needleman
Publikováno v:
eLife, Vol 9 (2020)
The spindle shows remarkable diversity, and changes in an integrated fashion, as cells vary over evolution. Here, we provide a mechanistic explanation for variations in the first mitotic spindle in nematodes. We used a combination of quantitative gen
Externí odkaz:
https://doaj.org/article/eb04c23e32e04443a9ab2e61aafb1e5c
Autor:
Gunar Fabig, Robert Kiewisz, Norbert Lindow, James A Powers, Vanessa Cota, Luis J Quintanilla, Jan Brugués, Steffen Prohaska, Diana S Chu, Thomas Müller-Reichert
Publikováno v:
eLife, Vol 9 (2020)
Chromosome segregation during male meiosis is tailored to rapidly generate multitudes of sperm. Little is known about mechanisms that efficiently partition chromosomes to produce sperm. Using live imaging and tomographic reconstructions of spermatocy
Externí odkaz:
https://doaj.org/article/d87dc370923a473883bd3f91dfebec32
Autor:
Maria Köckert, Chukwuebuka William Okafornta, Charlice Hill, Anne Ryndyk, Cynthia Striese, Thomas Müller-Reichert, Leocadia Paliulis, Gunar Fabig
Spermatogenesis leads to the formation of functional sperm cells. Here we have applied high-pressure freezing in combination with transmission electron microscopy (TEM) to study the ultrastructure of sperm development in subadult males of the praying
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::24f6b67f4a915ec917b7c71297f4618f
https://doi.org/10.1101/2023.04.16.537100
https://doi.org/10.1101/2023.04.16.537100
Publikováno v:
Journal of Cell Science. 136
In 1891, the existence of an X chromosome was noted for the first time. Hermann Henking was studying spermatocyte divisions of the firebug Pyrrhocoris apterus and observed that one chromosome behaved differently than all of the rest of the chromosome