Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Guillaume F, Chanfreau"'
Publikováno v:
STAR Protocols, Vol 1, Iss 3, Pp 100140- (2020)
Summary: We describe an RT-PCR protocol that allows high-resolution mapping of splicing products and isoforms using fluorescently labeled primers. Each species contains one fluorescent group allowing a direct comparison of the different isoforms desp
Externí odkaz:
https://doaj.org/article/1a9a61d101fb47ab99ccabe93afacf7d
Autor:
Kevin R. Roy, Jason Gabunilas, Dean Neutel, Michelle Ai, Joyce Samson, Guochang Lyu, Guillaume F. Chanfreau
Publikováno v:
bioRxiv
The fidelity of splice site selection is thought to be critical for proper gene expression and cellular fitness. In particular, proper recognition of 3′-splice site (3′SS) sequences by the spliceosome is a daunting task considering the low comple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::017b74fe0655d1b169bc46c24ea72513
https://europepmc.org/articles/PMC9882110/
https://europepmc.org/articles/PMC9882110/
Autor:
Yanru Liu, Samuel DeMario, Kevin He, Michelle R. Gibbs, Keaton W. Barr, Guillaume F. Chanfreau
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, vol 119, iss 31
Many small nucleolar RNAs (snoRNA)s are processed from introns of host genes, but the importance of splicing for proper biogenesis and the fate of the snoRNAs is not well understood. Here we show that inactivation of splicing factors or mutation of s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38862bb23aaee020aef55110b4649b20
https://escholarship.org/uc/item/8r56b5fc
https://escholarship.org/uc/item/8r56b5fc
Publikováno v:
RNA. :rna.079505.122
RT-PCR and northern blots have long been used to study RNA isoforms usage for single genes. Recently, advancements in long read sequencing have yielded unprecedented information about the usage and abundance of these RNA isoforms. However, visualizat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c32a0c7603484834e83a4e14b61a957a
https://doi.org/10.1261/rna.079505.122
https://doi.org/10.1261/rna.079505.122
Publikováno v:
Cell reports. 39(8)
The N
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::157b5bf828730b16e58b0f1e098b77a7
https://pubmed.ncbi.nlm.nih.gov/35613576
https://pubmed.ncbi.nlm.nih.gov/35613576
Publikováno v:
RNA
The expression of bromodomain-containing proteins that regulate chromatin structure and accessibility must be tightly controlled to ensure the appropriate regulation of gene expression. In the yeast S. cerevisiae, Bromodomain Factor 2 (BDF2) expressi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::08d64af72a08be9190fbb3fe35158752
https://pubmed.ncbi.nlm.nih.gov/34497070
https://pubmed.ncbi.nlm.nih.gov/34497070
Autor:
Tadashi Kawashima, Stephen Douglass, Jason Gabunilas, Matteo Pellegrini, Guillaume F Chanfreau
Publikováno v:
PLoS Genetics, Vol 10, Iss 4, p e1004249 (2014)
Saccharomyces cerevisiae has been used as a model system to investigate the mechanisms of pre-mRNA splicing but only a few examples of alternative splice site usage have been described in this organism. Using RNA-Seq analysis of nonsense-mediated mRN
Externí odkaz:
https://doaj.org/article/a42f1981e279433ebcd2a3a48115efb1
Publikováno v:
PLoS Genetics, Vol 7, Iss 6, p e1002163 (2011)
Nonsense-mediated mRNA decay (NMD) prevents the accumulation of transcripts bearing premature termination codons. Here we show that Saccharomyces cerevisiae NMD mutants accumulate 5'-extended RNAs (CD-CUTs) of many subtelomeric genes. Using the subte
Externí odkaz:
https://doaj.org/article/ad066ef59ca94ccfa97a84890575cf15
Autor:
Guillaume F, Chanfreau
Publikováno v:
The Enzymes. 41
Constitutive and regulated turnover of RNAs is necessary to eliminate aberrant RNA molecules and control the level of specific mRNAs to maintain homeostasis or to respond to signals in living cells. Modifications of nucleosides in specific RNAs are i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7832b3aa4afb7ea4e5593cc2db77c43e
https://pubmed.ncbi.nlm.nih.gov/28601225
https://pubmed.ncbi.nlm.nih.gov/28601225