Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Guillaume A. Lessard"'
Publikováno v:
Analytical Chemistry. 80:9830-9834
We demonstrate a custom confocal fluorescence-microscope that is capable of tracking individual quantum dots undergoing three-dimensional Brownian motion (diffusion coefficient ∼ 0.5 μm2/s) in environments with a signal-to-background ratio as low
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1e52f0eb9510ea04020651ae28aad037
https://doi.org/10.1021/ac8021899
https://doi.org/10.1021/ac8021899
Autor:
Peter M. Goodwin, Guillaume A. Lessard, Mary E. Phipps, James H. Werner, Nathan P. Wells, Patrick J. Cutler, Diane S. Lidke, Bridget S. Wilson
Publikováno v:
Nano letters. 10(11)
We report a method for tracking individual quantum dot (QD) labeled proteins inside of live cells that uses four overlapping confocal volume elements and active feedback once every 5 ms to follow three-dimensional molecular motion. This method has su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af983a33673114ed8d2a24959bde561a
https://pubmed.ncbi.nlm.nih.gov/20957984
https://pubmed.ncbi.nlm.nih.gov/20957984
Autor:
Guillaume A. Lessard, James H. Werner, Nathan P. Wells, Mary E. Phipps, Diane S. Lidke, Bridget S. Wilson, Patrick J. Cutler
Publikováno v:
Biophysical Journal. 98(3)
We have constructed a new confocal fluorescence-microscope that uses active feed-back and a unique spatial filter geometry to follow individual fluorescent quantum dots as they diffuse throughout 3 dimensional space at rates faster than most intracel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8f9b2bbefcbbb9d0c3c2e6a1deecf30
Autor:
James H. Werner, Peter M. Goodwin, Guillaume A. Lessard, Bridget S. Wilson, Diane S. Lidke, Mary E. Phipps, Nathan P. Wells
Publikováno v:
Single Molecule Spectroscopy and Imaging II.
The ability to follow and observe single molecules as they function in live cells represents a major milestone for molecular-cellular biology. Here we present a tracking microscope that is able to track quantum dots in three dimensions and simultaneo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3573d55d885ee908425783b484ec8351
https://doi.org/10.1117/12.809412
https://doi.org/10.1117/12.809412
Publikováno v:
Frontiers in Optics 2007/Laser Science XXIII/Organic Materials and Devices for Displays and Energy Conversion.
We describe new instrumentation developed in our lab (essentially a confocal microscope with feedback) capable of following 3 dimensional motion of single quantum dots moving at rates comparable to intracellular protein traffic (microns/sec).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::63b26f8cf9b0e29f6e5c2c31288895e5
https://doi.org/10.1364/fio.2007.jmc3
https://doi.org/10.1364/fio.2007.jmc3
Publikováno v:
Technical Digest. Summaries of papers presented at the Conference on Lasers and Electro-Optics. Postconference Edition. CLEO '99. Conference on Lasers and Electro-Optics (IEEE Cat. No.99CH37013).
Summary form only given. We demonstrate an apertureless near-field scanning microscope for fluorescence imaging with λ/20 resolution. The use of fluorescence allows artifact free imaging and provides a stringent test that the contrast mechanism is o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d6240c6e47719c3b2e4ce5ac095d8eb6
https://doi.org/10.1109/cleo.1999.834614
https://doi.org/10.1109/cleo.1999.834614
Publikováno v:
Frontiers in Optics.
The historical importance of optical microscopy to biological study is difficult to overstate. Optical techniques are relatively non-invasive and minimally perturbative both during sample preparation and measurement, enabling temporally resolved imag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::870ace2c30a889b82cc673c88a2485f6
https://doi.org/10.1364/fio.2003.wz2
https://doi.org/10.1364/fio.2003.wz2
Publikováno v:
Postconference Digest Quantum Electronics and Laser Science, 2003. QELS..
We measured the optical response of fluorescent beads to sharp metallic and semiconducting probes, revealing several underlying near-field interactions. Our results suggest that /spl sim/10 nanometer optical resolution with spectroscopic chemical sen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0f10f73f610d240b1db1ee934584e220
https://doi.org/10.1109/qels.2003.238509
https://doi.org/10.1109/qels.2003.238509
Publikováno v:
SPIE Proceedings.
Single molecule imaging with optical methods has become an important tool in biophysical studies. However, when imaging molecules at room temperature using far field optics, one can only resolve molecules that are separated by a distance greater than
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8e3acbb0ec01d7ad8c83355d3663f50d
https://doi.org/10.1117/12.383349
https://doi.org/10.1117/12.383349
We describe an apertureless near field microscope for imaging fluorescent samples. Optical contrast is generated by exploiting fluorescent quenching near a metallized atomic force microscope tip. This microscope has been used to image fluorescent lat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d98fe53069e0f6e5f8fe3b4cc8e42ad
https://resolver.caltech.edu/CaltechAUTHORS:YANapl00
https://resolver.caltech.edu/CaltechAUTHORS:YANapl00
