Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Guido, van Mierlo"'
Autor:
Olga Pushkarev, Guido van Mierlo, Judith Franziska Kribelbauer, Wouter Saelens, Vincent Gardeux, Bart Deplancke
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-32 (2024)
Abstract Background Interactions among cis-regulatory elements (CREs) play a crucial role in gene regulation. Various approaches have been developed to map these interactions genome-wide, including those relying on interindividual epigenomic variatio
Externí odkaz:
https://doaj.org/article/c50711ddf2284a3dbc0ccd08db72a7ee
Autor:
Juan Manuel Valverde, Geronimo Dubra, Michael Phillips, Austin Haider, Carlos Elena-Real, Aurélie Fournet, Emile Alghoul, Dhanvantri Chahar, Nuria Andrés-Sanchez, Matteo Paloni, Pau Bernadó, Guido van Mierlo, Michiel Vermeulen, Henk van den Toorn, Albert J. R. Heck, Angelos Constantinou, Alessandro Barducci, Kingshuk Ghosh, Nathalie Sibille, Puck Knipscheer, Liliana Krasinska, Daniel Fisher, Maarten Altelaar
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-23 (2023)
Abstract Cell cycle transitions result from global changes in protein phosphorylation states triggered by cyclin-dependent kinases (CDKs). To understand how this complexity produces an ordered and rapid cellular reorganisation, we generated a high-re
Externí odkaz:
https://doaj.org/article/e9908b9e43c84a22ae719efb78f9bc6b
Autor:
Joern Pezoldt, Carolin Wiechers, Mangge Zou, Maria Litovchenko, Marjan Biocanin, Michael Beckstette, Katarzyna Sitnik, Martina Palatella, Guido van Mierlo, Wanze Chen, Vincent Gardeux, Stefan Floess, Maria Ebel, Julie Russeil, Panagiota Arampatzi, Ehsan Vafardanejad, Antoine-Emmanuel Saliba, Bart Deplancke, Jochen Huehn
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-19 (2022)
Lymph nodes in various locations of the body differ in their cell composition and gene expression signatures. Here authors show that the rapid postnatal expansion of lymph nodes is governed by CD34 + stromal cells and fibroblastic reticular stromal c
Externí odkaz:
https://doaj.org/article/a6cebd9a2df24f8cacb6093aef1fff24
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling in
Externí odkaz:
https://doaj.org/article/daee1039e77c42bc92b128ce138c9f8d
Autor:
Menno ter Huurne, Tianran Peng, Guoqiang Yi, Guido van Mierlo, Hendrik Marks, Hendrik G. Stunnenberg
Publikováno v:
Stem Cell Reports, Vol 14, Iss 2, Pp 175-183 (2020)
Summary: Mouse embryonic stem cells (ESCs) grown in serum-supplemented conditions are characterized by an extremely short G1 phase due to the lack of G1-phase control. Concordantly, the G1-phase-specific P53-P21 pathway is compromised in serum ESCs.
Externí odkaz:
https://doaj.org/article/c34d3da9991040b2b6484a63cb6542cf
Autor:
Dirk J. W. den Braanker, Rutger J. H. Maas, Guido van Mierlo, Naomi M. J. Parr, Marinka Bakker-van Bebber, Jeroen K. J. Deegens, Pascal W. T. C. Jansen, Jolein Gloerich, Brigith Willemsen, Henry B. Dijkman, Alain J. van Gool, Jack F. M. Wetzels, Markus M. Rinschen, Michiel Vermeulen, Tom Nijenhuis, Johan van der Vlag
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 1, p 194 (2022)
Many patients with primary focal segmental glomerulosclerosis (FSGS) develop recurrence of proteinuria after kidney transplantation. Several circulating permeability factors (CPFs) responsible for recurrence have been suggested, but were never valida
Externí odkaz:
https://doaj.org/article/8113e821834d47ac89121d081ec8b529
Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states
Autor:
René A. M. Dirks, Guido van Mierlo, Hindrik H. D. Kerstens, Andreia S. Bernardo, Julianna Kobolák, István Bock, Julien Maruotti, Roger A. Pedersen, András Dinnyés, Martijn A. Huynen, Alice Jouneau, Hendrik Marks
Publikováno v:
Epigenetics & Chromatin, Vol 12, Iss 1, Pp 1-21 (2019)
Abstract Background Genomic imprinting, resulting in parent-of-origin specific gene expression, plays a critical role in mammalian development. Here, we apply allele-specific RNA-seq on isogenic B6D2F1 mice to assay imprinted genes in tissues from ea
Externí odkaz:
https://doaj.org/article/ccab5f203e284c20b12ae65e88095907
Publikováno v:
Stem Cell Research, Vol 33, Iss , Pp 135-145 (2018)
The ground state of pluripotency is defined as a minimal unrestricted epigenetic state as present in the Inner Cell Mass. Mouse embryonic stem cells (ESCs) grown in a defined serum-free medium with two kinase inhibitors (“2i ESCs”) have been post
Externí odkaz:
https://doaj.org/article/6190e8a159ec4c3db3391450e6862150
Autor:
Guus H. van Muijlwijk, Guido van Mierlo, Pascal W.T.C. Jansen, Michiel Vermeulen, Nancy M.C. Bleumink-Pluym, Noah W. Palm, Jos P.M. van Putten, Marcel R. de Zoete
Publikováno v:
Gut Microbes, Vol 13, Iss 1 (2021)
The human gut microbiota plays a central role in intestinal health and disease. Yet, many of its bacterial constituents are functionally still largely unexplored. A crucial prerequisite for bacterial survival and proliferation is the creation and/or
Externí odkaz:
https://doaj.org/article/9ee661d020064bee903df301594f96e2
Publikováno v:
Nature Protocols, 18, pp. 36-57
Nature Protocols, 18, 36-57
Nature Protocols, 18, 36-57
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or clustered regularly interspaced