Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Guan-Zhen Yang"'
Publikováno v:
Acta Biochimica et Biophysica Sinica
The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients.
Publikováno v:
Molecular Biotechnology. 24:21-26
BmK ITa1 is an insect-specific neurotoxin from the Chinese scorpion Buthus martensi Karsch (Bmk). We succeeded in obtaining biologically active recombinant BmK ITa1 protein by simultaneous expression in insect cells of BmK ITa1 cDNA with an amidating
Publikováno v:
Protein & Peptide Letters. 9:323-329
A synthetic modified gene encoding the human calcitonin analog (hmCT) was expressed by use of the baculovirus expression system. After injection with recombinant baculoviruses, the hmCT-GST fusion protein was produced within the silkworm larvae. The
Publikováno v:
Protein Expression and Purification. 24:13-17
Much is known about the physical properties of the Cu,Zn- and Mn-superoxide dismutases (SODs). However, the biochemical characteristics and pharmacological properties of extracellular (EC)-SOD have been severely limited due to difficulties in obtaini
Publikováno v:
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitologyparasitic diseases. 26(2)
To express the soluble recombinant Schistosoma japonicum SjPP proteins in TN5B1-4 cells.The total RNA was extracted from adult worms of Schistosoma japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plas
Publikováno v:
Protein & Peptide Letters. 7:233-240
Abstract: A novel osteoprotegerin (OPG) isoform cDNA was cloned and expressed in the insect cell and yeast. No homodimeric OPG was found according to the SDS-PAGE analysis and western blot. The OPG isoform was 8 amino acid residues less than the repo
Publikováno v:
Journal of cellular biochemistry. 99(6)
Previous work in our laboratory has shown agkistin, a snake venom metalloproteases (SVMPs) from the venom of Agkistrodon halys, possesses antiplatelet aggregation activity. In this study, we further examined the antiangiogenic activity of agkistin-s,
Publikováno v:
Protein and peptide letters. 11(4)
Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is pr
Publikováno v:
Molecular biotechnology. 27(1)
In the proprotein convertases family, mouse PC6B (mPC6B) has a very large cysteine-rich region (CRR), consisting of 22 tandem cysteine-rich (Cys-rich) repeated segments. The role of this region remains elusive. In this report, to get insight on the p
Publikováno v:
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica. 35(10)
Ribonuclease inhibitor (RI) is an acidic 50 kD protein with a high content of leucine and cysteine residues. RI inhibits RNases of the pancreatic type. A variant of RI was cloned from human fetal liver cDNA library by polymerase chain reaction (PCR).