EcoRII Restriction Endonuclease Forms Specific Contacts to the Bases of Its Target Sequence Flipped from DNA in a Transition Complex with Photoactivatable Substrates

Autor: R. I. Eritja, O. V. Kirsanova, Fedor V. Subach, Gromova Es, A. G. Loiko

Publikováno v: Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Digital.CSIC. Repositorio Institucional del CSIC
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The photoactivatable modified oligonucleotides were used to investigate direct contacts formed by the type IIE EcoRII restriction endonuclease and the T/A bases of its recognition site (5'-CCT/AGG). EcoRII dimer consists of a central catalytic core,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52707d4bfe237ce3eacfaefc41a8e319
http://hdl.handle.net/10261/242558

DNA Duplexes Containing Altered Sugar Residues as Probes ofEcoRII andMvaI Endonuclease Interactions with Sugar-Phosphate Backbone

Autor: Ya. I. Alekseev, Fedor V. Subach, O. V. Babkina, Gromova Es, Olga Petrauskene, J. N. Yakovleva

Publikováno v: Journal of Biomolecular Structure and Dynamics. 17:857-870

Oligonucleotides containing 1-(beta-D-2'-deoxy-threo-pentofuranosyl)cytosine (dCx) and/or 1-(beta-D-2'-deoxy-threo-pentofuranosyl)thymine (dTx) in place of dC and dT residues in the EcoRII and MvaI recognition site CC(A/T)GG were synthesized in order

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54bd2386a9e73b1364a5fffccde2ac1e
https://doi.org/10.1080/07391102.2000.10506574

EcoRII endonuclease has two identical DNA-binding sites and cleaves one of two co-ordinated recognition sites in one catalytic event

Autor: Vadim N. Tashlitsky, O. V. Babkina, Olga Petrauskene, Gromova Es, Gregory M. Kazankov

Publikováno v: FEBS Letters. 425:29-34

EcoRII is a typical restriction enzyme that cleaves DNA using a two-site mechanism. EcoRII endonuclease is unable to cleave DNA which contains a small number of EcoRII recognition sites but the enzyme activity can be stimulated in the presence of DNA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80cd548ec01b8e843f552770c6e42741
https://doi.org/10.1016/s0014-5793(98)00184-7

Modified substrates as probes for studying uracil-DNA glycosylase

Autor: T. S. Oretskaya, E. M. Volkov, Maxim G. Brevnov, Natalya L. Vinogradova, Zoe A. Shabarova, Elena A. Kubareva, Gromova Es, Georgy A. Nevinsky, Igor A. Kanevsky, S. A. Kuznetsova

Publikováno v: Gene. 157(1-2)

In order to study the mechanism of action of uracil-DNA glycosylase (UDG) from human placenta, single-stranded (ss) and double-stranded (ds) oligodeoxyribonucleotides (oligos), containing deoxyuridine (dU) and a wide variety of their analogs were use

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::985709bfb3321f3dab316a296b4af7c2
https://pubmed.ncbi.nlm.nih.gov/7607485

DNA-Protein Interactions: The Use of Synthetic Oligo-and Polynucleotides for Studying Single-stranded-DNA-binding Proteins and Restriction Endonucleases

Autor: Gromova Es, Zoe A. Shabarova

Publisher Summary This chapter discusses the interactions of proteins with synthesized fragments of DNA by convenient physicochemical and enzymological methods. It defines such a comprehensive approach as an organochemical one. Strict positional dire

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::baa7b87e6b90ababcb6c9147d3db7a4c
https://doi.org/10.1016/s0079-6603(08)60622-4