Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Greta Bigelyte"'
Autor:
Greta Bigelyte, Joshua K. Young, Tautvydas Karvelis, Karolina Budre, Rimante Zedaveinyte, Vesna Djukanovic, Elizabeth Van Ginkel, Sushmitha Paulraj, Stephen Gasior, Spencer Jones, Lanie Feigenbutz, Grace St. Clair, Pierluigi Barone, Jennifer Bohn, Ananta Acharya, Gina Zastrow-Hayes, Selgar Henkel-Heinecke, Arunas Silanskas, Ralf Seidel, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Miniature Cas12f editing systems are well suited for in vivo editing applications. Here the authors characterize the intrinsic activity of SpCas12f1 in plant and animal cells.
Externí odkaz:
https://doaj.org/article/688a63ea2c9f45e084a40d762048f444
Autor:
Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available to
Externí odkaz:
https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
Autor:
Česlovas Venclovas, Gytis Druteika, Darius Kazlauskas, Arunas Silanskas, Virginijus Siksnys, Rimante Zedaveinyte, Karolina Budre, Tautvydas Karvelis, Greta Bigelyte
Publikováno v:
Nature, Berlin : Nature research, 2021, vol. 599, p. 692-696
Transposition has a key role in reshaping genomes of all living organisms1. Insertion sequences of IS200/IS605 and IS607 families2 are among the simplest mobile genetic elements and contain only the genes that are required for their transposition and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51bf5a25af0ec559da51d1bb5a09fafe
https://doi.org/10.1038/s41586-021-04058-1
https://doi.org/10.1038/s41586-021-04058-1
Autor:
Joshua K. Young, William E. Jack, Česlovas Venclovas, Clara M. Alarcon, Darius Kazlauskas, Zhenglin Hou, Virginijus Siksnys, Megumu Mabuchi, Tomas Urbaitis, Tautvydas Karvelis, G. Brett Robb, Mark Cigan, Shane K. Dooley, Monika Jasnauskaite, Giedrius Gasiunas, Ryan T. Fuchs, N. Doane Chilcoat, Greta Bigelyte, Mantvyda M. Grusyte, Ezra Schildkraut, Po-Hao Wang, Peter Weigele, Sushmitha Paulraj, Zhiyi Sun, Jennifer L. Curcuru
CRISPR-Cas9 nucleases are abundant in microbes. To explore this largely uncharacterized diversity, we applied cell-free biochemical screens to rapidly assess the protospacer adjacent motif (PAM) and guide RNA (gRNA) requirements of novel Cas9 protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f6cdbeb4449b33160674b8f8cb468bb
https://doi.org/10.1101/2020.04.29.066654
https://doi.org/10.1101/2020.04.29.066654
Autor:
Zhenglin Hou, Rimante Zedaveinyte, Joshua K. Young, Sushmitha Paulraj, Arunas Silanskas, Greta Bigelyte, Virginijus Siksnys, Vesna Djukanovic, Karolina Budre, Tautvydas Karvelis, Česlovas Venclovas, Stephen L. Gasior
Publikováno v:
Nucleic acids research, Oxford : Oxford University Press, 2020, first on line, p. [1-8]
Nucleic acids research, Oxford : Oxford University Press, 2020, vol. 48, iss. 9, p. 5016-5023
Nucleic Acids Research
Nucleic acids research, Oxford : Oxford University Press, 2020, vol. 48, iss. 9, p. 5016-5023
Nucleic Acids Research
In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d247b0d5509d3ee33c07486670fba61
https://repository.vu.lt/VU:ELABAPDB55056678&prefLang=en_US
https://repository.vu.lt/VU:ELABAPDB55056678&prefLang=en_US
Autor:
Zhenglin Hou, Česlovas Venclovas, Peter Weigele, Megumu Mabuchi, Joshua K. Young, William E. Jack, Zhiyi Sun, Greta Bigelyte, Tautvydas Karvelis, Ezra Schildkraut, Virginijus Siksnys, N. Doane Chilcoat, Tomas Urbaitis, G. Brett Robb, Ryan T. Fuchs, Monika Jasnauskaite, Jennifer L. Curcuru, Mantvyda M. Grusyte, Shane K. Dooley, Po-Hao Wang, Giedrius Gasiunas, Clara M. Alarcon, Darius Kazlauskas, Mark Cigan, Sushmitha Paulraj
Publikováno v:
Nature communications, London : Nature Publishing Group, 2020, vol. 11, no. 1, art. no. 5512, p. [1-10]
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been repurposed as genome editing tools. Although these proteins are found in many microbes, only a handful of variants are used for these applications. Here, we use bioi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c339dbfa2e08fc6e4b32aa0ea45d7144
https://repository.vu.lt/VU:ELABAPDB74530963&prefLang=en_US
https://repository.vu.lt/VU:ELABAPDB74530963&prefLang=en_US
Publikováno v:
Biophysical Journal. 121:477a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::088a2a81a5a108ae55415ee814288c80
https://doi.org/10.1016/j.bpj.2021.11.375
https://doi.org/10.1016/j.bpj.2021.11.375
Autor:
Stephen L. Gasior, Zhenglin Hou, Virginijus Siksnys, Greta Bigelyte, Vesna Djukanovic, Joshua K. Young, Arunas Silanskas, Česlovas Venclovas, Rimante Zedaveinyte, Sushmitha Paulraj, Karolina Budre, Tautvydas Karvelis
In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ae7b83f3950dd003dc996178de31ff0
https://doi.org/10.1101/654897
https://doi.org/10.1101/654897
Autor:
Joshua Young, Arunas Silanskas, Giedrius Gasiunas, Virginijus Siksnys, Tautvydas Karvelis, A. Mark Cigan, Greta Bigelyte
Publikováno v:
Genome Biology
Genome biology, London : BioMed Central Ltd., 2015, vol. 16, art. no. 253, p. [1-13]
Genome biology, London : BioMed Central Ltd., 2015, vol. 16, art. no. 253, p. [1-13]
To expand the repertoire of Cas9s available for genome targeting, we present a new in vitro method for the simultaneous examination of guide RNA and protospacer adjacent motif (PAM) requirements. The method relies on the in vitro cleavage of plasmid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5c020aaf795477ae88eba2215c62612
https://doi.org/10.1186/s13059-015-0818-7
https://doi.org/10.1186/s13059-015-0818-7
Autor:
Karolina Budre, Stephen L. Gasior, Selgar Henkel-Heinecke, Tautvydas Karvelis, Ralf Seidel, Virginijus Siksnys, Vesna Djukanovic, Rimante Zedaveinyte, Spencer Jones, Sushmitha Paulraj, Arunas Silanskas, Elizabeth Van Ginkel, Grace St. Clair, Greta Bigelyte, Pierluigi Barone, Gina Zastrow-Hayes, Ananta Acharya, Jennifer A. Bohn, Joshua K. Young, Lanie Feigenbutz
Publikováno v:
Nature communications, Berlin : Nature research, 2021, vol. 12, iss. 1, art. no. 6191, p. [1-8]
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these CRISPR-associated (Cas) nucleases ranges from >1000 amino acids
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a52bb3de9810691d01aee8bf73372b5d