Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Gregory W. deHart"'
Publikováno v:
Journal of Visualized Experiments : JoVE
The administration of enzyme replacement therapies (ERTs) and other biologic therapies to patients may elicit an anti-drug immune response. The characterization of these anti-drug antibodies (ADA), especially those that may neutralize the biological
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fcaea2f9ea9c9abd5ba55aeb7197975
http://europepmc.org/articles/PMC6235114
http://europepmc.org/articles/PMC6235114
Publikováno v:
Nature Protocols. 7:1052-1067
We describe a method for modifying proteins site-specifically using a chemoenzymatic bioconjugation approach. Formylglycine generating enzyme (FGE) recognizes a pentapeptide consensus sequence, CxPxR, and it specifically oxidizes the cysteine in this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85ec88bdf2923b391155305fa8c292ae
https://doi.org/10.1038/nprot.2012.045
https://doi.org/10.1038/nprot.2012.045
Autor:
Russell K. Soon, J. Genevieve Park, Andrew C. Melton, Luis Martinez, Hal F. Yee, Gregory W. deHart
Publikováno v:
American Journal of Physiology-Gastrointestinal and Liver Physiology. 293:G1272-G1280
Chemotaxis (i.e., directed migration) of hepatic stellate cells to areas of inflammation is a requisite event in the liver's response to injury. Previous studies of signaling pathways that regulate stellate cell migration suggest a key role for focal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1415ff4cdb3f643c69e5e8ff6dfd2c05
https://doi.org/10.1152/ajpgi.00134.2007
https://doi.org/10.1152/ajpgi.00134.2007
Publikováno v:
Experimental Cell Research. 283:67-79
Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e274dd5b710bf1405ee81fb5d7bc079
https://doi.org/10.1016/s0014-4827(02)00028-9
https://doi.org/10.1016/s0014-4827(02)00028-9
Autor:
Gregory W. deHart, Lawrence E. Goldfinger, Sherry S. Collawn, J. R. Couchman, Susan B. Hopkinson, Jonathan C.R. Jones
Publikováno v:
Journal of Cell Science. 112:2615-2629
Previously, we demonstrated that proteolytic processing within the globular domain of the alpha3 subunit of laminin-5 (LN5) converts LN5 from a cell motility-inducing factor to a protein complex that can trigger the formation of hemidesmosomes, certa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68c5ae81cfae42c1d769d31dc3491122
https://doi.org/10.1242/jcs.112.16.2615
https://doi.org/10.1242/jcs.112.16.2615
Publikováno v:
Journal of Investigative Dermatology. 111:1015-1022
The hemidesmosome is a multimolecular complex that integrates the extracellular matrix with the keratin cytoskeleton and that stabilizes epithelial attachment to connective tissue. A 180 kDa protein (BP180, type XVII collagen), first identified by it
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1981e538bbc484b633cd227d8bbd541
https://doi.org/10.1046/j.1523-1747.1998.00452.x
https://doi.org/10.1046/j.1523-1747.1998.00452.x
Publikováno v:
Microscopy Research and Technique. 51:211-213
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::60af935eb70c96b7264ba95b44be6851
https://doi.org/10.1002/1097-0029(20001101)51:3<211::aid-jemt1>3.0.co
https://doi.org/10.1002/1097-0029(20001101)51:3<211::aid-jemt1>3.0.co
Transdominant inhibition of integrins or integrin-integrin crosstalk is an important regulator of integrin ligand binding and subsequent signaling events that control a variety of cell functions in many tissues. Here we discuss examples of integrin c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6d8b33bc38852afd63cfff18f15b51f
https://europepmc.org/articles/PMC2814898/
https://europepmc.org/articles/PMC2814898/
Autor:
Jonathan C.R. Jones, Gregory W. deHart
Publikováno v:
Cell motility and the cytoskeleton. 57(2)
Laminin-5 is a major structural element of epithelial tissue basement membranes. In the matrix of cultured epithelial cells, laminin-5 is arranged into intricate patterns. Here we tested a hypothesis that myosin II-mediated actin contraction is neces
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea06ad0792a2bafffd28aead484767ec
https://pubmed.ncbi.nlm.nih.gov/14691950
https://pubmed.ncbi.nlm.nih.gov/14691950
Publikováno v:
Cell Motility & the Cytoskeleton; Feb2004, Vol. 57 Issue 2, p107-117, 11p