Zobrazeno 1 - 10
of 128
pro vyhledávání: '"Gregory Timp"'
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)
Abstract The blockade current that develops when a protein translocates across a thin membrane through a sub-nanometer diameter pore informs with extreme sensitivity on the sequence of amino acids that constitute the protein. The current blockade sig
Externí odkaz:
https://doaj.org/article/6a5f673504ca47f9a5364feaa30b0828
Autor:
Eveline Rigo, Zhuxin Dong, Jae Hyun Park, Eamonn Kennedy, Mohammad Hokmabadi, Lisa Almonte-Garcia, Li Ding, Narayana Aluru, Gregory Timp
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
The size of an ion affects everything from the structure of water to life itself. Here, a sub-nanometer diameter pore sputtered through a thin silicon nitride membrane is used to systematically test ion permeability by measuring the electrolytic curr
Externí odkaz:
https://doaj.org/article/7fc0f89ead0148c2a122d3aa0aec5300
Publikováno v:
PLoS Computational Biology, Vol 13, Iss 5, p e1005356 (2017)
Recent advances in top-down mass spectrometry enabled identification of intact proteins, but this technology still faces challenges. For example, top-down mass spectrometry suffers from a lack of sensitivity since the ion counts for a single fragment
Externí odkaz:
https://doaj.org/article/c9c3deff1b50448ca7dd887c4a9b0076
Publikováno v:
IEEE Access, Vol 2, Pp 1396-1408 (2014)
It is now possible to manipulate individual molecules using a nanopore to read DNA and proteins, or write DNA by inserting mini-genes into cells. Furthermore, development of these methodologies will kick open the door to new biology and chemistry tha
Externí odkaz:
https://doaj.org/article/935958f0afd047298b9c9727132ed1a0
Autor:
Tautvydas Lisauskas, Archith Rayabharam, Punam Murkute, Lisa Almonte, Eveline Rigo, Juan Oviedo Robles, Zhuxin Dong, Joshy Joseph, Narayana Aluru, Gregory Timp
When a denatured protein isoform (i.e., a proteoform) immersed in electrolyte is impelled by an electric field through a sub-nanometer-diameter pore (i.e., a sub-nanopore) spanning a thin membrane, the sequence of amino acid (AA) residues constitutin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2da4cd72df1bd3e81de9d46b329ae4ce
https://doi.org/10.1101/2022.12.22.521660
https://doi.org/10.1101/2022.12.22.521660
Publikováno v:
ACS Nano. 11:3-7
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7834ac98c315f82b03868046d8334903
https://doi.org/10.1021/acsnano.6b06616
https://doi.org/10.1021/acsnano.6b06616
Autor:
Jae Hyun Park, Narayana R. Aluru, Lisa Almonte-Garcia, Mohammad Hokmabadi, Li Ding, Zhuxin Dong, E. Rigo, Gregory Timp, Eamonn Kennedy
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications
Nature Communications
The size of an ion affects everything from the structure of water to life itself. In this report, to gauge their size, ions dissolved in water are forced electrically through a sub-nanometer-diameter pore spanning a thin membrane and the current is m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::873e32e87f8d08a7dbb03ad507c64216
https://doi.org/10.1038/s41467-019-10265-2
https://doi.org/10.1038/s41467-019-10265-2
Publikováno v:
Nature Nanotechnology. 11:968-976
The primary structure of a protein consists of a sequence of amino acids and is a key factor in determining how a protein folds and functions. However, conventional methods for sequencing proteins, such as mass spectrometry and Edman degradation, suf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::52f46c68fc741f9f293c865714946e1e
https://doi.org/10.1038/nnano.2016.120
https://doi.org/10.1038/nnano.2016.120
Publikováno v:
ACS Synthetic Biology. 5:1421-1432
The promise of adapting biology to information processing will not be realized until engineered gene circuits, operating in different cell populations, can be wired together to express a predictable function. Here, elementary biological integrated ci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::500bd77760152748ea12fee6d5f49657
https://doi.org/10.1021/acssynbio.6b00002
https://doi.org/10.1021/acssynbio.6b00002
Autor:
Kim McKelvey, Edward M. Nelson, Gregory Timp, Zhuxin Dong, Mohammad Hokmabadi, Eamonn Kennedy
Publikováno v:
Nano letters. 18(7)
Secreted proteins mediate cell-to-cell communications. Thus, eavesdropping on the secretome could reveal the cellular phenotype, but it is challenging to detect the proteins because they are secreted only in minute amounts and then diluted in blood p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cbace8f24d885e513bf9a95d90c6f0c
https://pubmed.ncbi.nlm.nih.gov/29870666
https://pubmed.ncbi.nlm.nih.gov/29870666