Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Gregory S. Blank"'
Autor:
Qi Chen, Gregory S. Blank, Scott Lute, Bin Yang, Daniel Strauss, Douglas D. Frey, Cintia Ho, Kurt Brorson, Zinaida Tebaykina
Publikováno v:
Biotechnology and Bioengineering. 104:371-380
During production of therapeutic monoclonal antibodies (mAbs) in mammalian cell culture, it is important to ensure that viral impurities and potential viral contaminants will be removed during downstream purification. Anion exchange chromatography pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d1e6d07a4826c949b669c73a2800673
https://doi.org/10.1002/bit.22416
https://doi.org/10.1002/bit.22416
Publikováno v:
Biotechnology Progress. 25:1194-1197
Retrovirus-like particles (RVLPs) that are expressed during the production of monoclonal antibodies in Chinese hamster ovary (CHO) cell cultures must be removed during product recovery. Anion exchange chromatography (AEX) performed in product flow-th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecf904f34a1669e25620349fd1634e1a
https://doi.org/10.1002/btpr.249
https://doi.org/10.1002/btpr.249
Publikováno v:
Biotechnology and Bioengineering. 102:168-175
The mammalian cell-lines used to produce biopharmaceutical products are known to produce endogenous retrovirus-like particles and have the potential to foster adventitious viruses as well. To ensure product safety and regulatory compliance, recovery
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89269912a2dc3e28e3c370206309d61a
https://doi.org/10.1002/bit.22051
https://doi.org/10.1002/bit.22051
Publikováno v:
Journal of Chromatography A. 830:127-134
Acetonitrile is used as an eluent for reversed-phase chromatography. However, because it is a flammable solvent, using acetonitrile on a large scale requires expensive equipment and facilities specially designed for flammable solvents. Using a non-fl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c59195b91d1600321abd852697503ccc
https://doi.org/10.1016/s0021-9673(98)00866-8
https://doi.org/10.1016/s0021-9673(98)00866-8
Publikováno v:
Journal of Chromatography A. 827:37-43
During preparative reversed-phase chromatography of recombinant human insulin-like growth factor-I (IGF), the separation of IGF from IGF aggregates cannot be determined using UV absorbance. An on-line reversed-phase chromatographic assay was develope
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c038ad87d99728523c65434c88d800fa
https://doi.org/10.1016/s0021-9673(98)00778-x
https://doi.org/10.1016/s0021-9673(98)00778-x
Publikováno v:
Biotechnology and Bioengineering. 45:149-157
There are three major methods for buffer exchange of proteins at industrial scale: size exclusion chromatography (SEC), tangential flow filtration (TFF), and countercurrent dialysis (CCD). In order to determine the optimal technology for a given proc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::803c2bba3c6cbf270e5bd04f1ac57fde
https://doi.org/10.1002/bit.260450209
https://doi.org/10.1002/bit.260450209
Autor:
Daniel Strauss, Tony Cano, Daniel Coleman, Qi Chen, Gregory S. Blank, Nick Cai, Magdalena Plancarte, Bin Yang, Heather Delucchi
Publikováno v:
Biotechnology progress. 26(3)
The quality-by-design (QbD) regulatory initiative promotes the development of process design spaces describing the multidimensional effects and interactions of process variables on critical quality attributes of therapeutic products. However, because
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dc46b16bf79444164f22f5d1b1137d0
https://pubmed.ncbi.nlm.nih.gov/20306523
https://pubmed.ncbi.nlm.nih.gov/20306523
Publikováno v:
Biotechnology and bioengineering. 84(2)
The potential of viral contamination is a regulatory concern for continuous cell line-derived pharmaceutical proteins. Complementary and redundant safety steps, including an evaluation of the viral clearance capacity of unit operations in the purific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0ac095cd85b07f1c2ab8b72adf19a32
https://pubmed.ncbi.nlm.nih.gov/12966574
https://pubmed.ncbi.nlm.nih.gov/12966574
Publikováno v:
Scale-Up and Optimization in Preparative Chromatography: Principles and Biopharmaceutical Applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::35cdeded5cbc2f8201d5b214f6b58362
https://doi.org/10.1201/9780203909287.ch7
https://doi.org/10.1201/9780203909287.ch7
Autor:
Gregory S. Blank, Robert L. Fahrner
Publikováno v:
Methods for Affinity-Based Separations of Enzymes and Proteins ISBN: 9783764363055
Perfusion chromatography is an important tool for rapid separations of biomolecules. Introduced more than a decade ago [1-3], perfusion chromatography media are polymeric (polystyrene-divinylbenzene) and contain large through-pores that completely pe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8bd14a3748cb704345af3798405b7540
https://doi.org/10.1007/978-3-0348-8127-2_4
https://doi.org/10.1007/978-3-0348-8127-2_4