A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes†

Autor: Anthony P. Green, Andrea Marchesi, Jolanda Van Van Munster, Helene Ledru, Sabine L. Flitsch, M. Carmen Galan, Lisa S. Seibt, Fabio Parmeggiani, Peter Both, Gregory S Bulmer, Kun Huang, Ashley P. Mattey, Ryan Williams

Publikováno v: Organic & Biomolecular Chemistry
Bulmer, G S, Mattey, A P, Parmeggiani, F, Williams, R, Ledru, H, Marchesi, A, Seibt, L S, Both, P, Huang, K, Galan, M C, Flitsch, S L, Green, A P & van Munster, J M 2021, ' A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes ', Organic and Biomolecular Chemistry, vol. 19, no. 25, pp. 5529-5533 . https://doi.org/10.1039/d1ob00971k
Bulmer, G S, Mattey, A P, Parmeggiani, F, Williams, R, Ledru, H, Marchesi, A, Seibt, L, Both, P, Huang, K, Galan, M C, Flitsch, S L, Green, A & Van Munster, J V 2021, ' A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes ', Organic & biomolecular chemistry . https://doi.org/10.1039/D1OB00971K

Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucose via the generation of β-1,4-glycosidic linkages. The biocatalyst was incorporated into biocatalytic cascades and chemo-enzymatic strategies to synthes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8675763d78a0cdead20edbc4b2ac3bed
http://europepmc.org/articles/PMC8243248

The feruloyl esterase from Thermobacillus xylanilyticus shows broad specificity for processing pre-biotic feruloylated xylooligosaccharides at high temperatures

Autor: Carolina V. Garbelotti, Gregory S. Bulmer, Richard J. Ward, Jolanda M. van Munster

Publikováno v: Food chemistry. 405

Ferulic acid has antioxidant properties of interest to the food industry and can be released from natural plant fibres using feruloyl esterases. Esterases active at high temperatures are highly desirable but currently underrepresented. Here we report

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54efff222339ae50c7f599ccf35c945b
https://pubmed.ncbi.nlm.nih.gov/36403470

Selective Oxidation of N-Glycolylneuraminic Acid Using an Engineered Galactose Oxidase Variant

Autor: William R. Birmingham, Sabine L. Flitsch, Juana Elizabeth Reyes Martinez, Fabio Parmeggiani, Nico Kress, Josef Voglmeir, Nicholas J. Turner, Kun Huang, Ashley P. Mattey, Peter Both, Gregory S Bulmer, Jolanda M. van Munster

Publikováno v: Mattey, A, Birmingham, W R, Both, P, Kress, N, Huang, K, Van Munster, J M, Bulmer, G, Parmeggiani, F, Voglmeir, J, Reyes Martinez, J E, Turner, N J & Flitsch, S L 2019, ' Selective Oxidation of N-Glycolylneuraminic Acid Using an Engineered Galactose Oxidase Variant ', ACS Catalysis, vol. 9, no. 9, pp. 8208-8212 . https://doi.org/10.1021/acscatal.9b02873

N-Glycolylneuraminic acid (Neu5Gc) is a common cell surface ligand in animals which is not biosynthesized in humans but can be acquired in human tissue from dietary sources such as red meat. It is important to understand the relevance of this potenti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99ef22d488fc4681a7072827bcf7a3ce
https://www.research.manchester.ac.uk/portal/en/publications/selective-oxidation-of-nglycolylneuraminic-acid-using-an-engineered-galactose-oxidase-variant(211706b8-d90f-4952-b533-24ba045e4689).html

Enzymatic synthesis of N -acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Autor: Helene Ledru, Jolanda M. van Munster, Peter Both, Ashley P. Mattey, Gregory S Bulmer, Kristian Hollingsworth, W. Bruce Turnbull, M. Carmen Galan, Fabio Parmeggiani, Andrea Marchesi, Jordi Mas Pons, Sabine L. Flitsch, Edward Pallister, Kun Huang

Publikováno v: Organic & Biomolecular Chemistry
Huang, K, Parmeggiani, F, Ledru, H, Hollingsworth, K, Pons, J M, Marchesi, A, Both, P, Mattey, A P, Pallister, E, Bulmer, G S, Munster, J M V, Turnbull, W B, Galan, M C & Flitsch, S L 2019, ' Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases ', Organic & biomolecular chemistry . https://doi.org/10.1039/C9OB01089K
Huang, K, Parmeggiani, F, Ledru, H, Hollingsworth, K, Mas Pons, J, Marchesi, A, Both, P, Mattey, A P, Pallister, E, Bulmer, G S, van Munster, J M, Turnbull, W B, Galan, C & Flitsch, S L 2019, ' Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases ', Organic and Biomolecular Chemistry, vol. 17, no. 24, pp. 5920-5924 . https://doi.org/10.1039/C9OB01089K

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1de6b0ff8f567465bb690a6be2b2551c

Recent advances in enzymatic synthesis of β-glucan and cellulose

Autor: Peterson de Andrade, Jolanda M. van Munster, Robert A. Field, Gregory S Bulmer

Publikováno v: University of Manchester-PURE
Carbohydrate Research

Bottom-up synthesis of β-glucans such as callose, fungal β-(1,3)(1,6)-glucan and cellulose, can create the defined compounds that are needed to perform fundamental studies on glucan properties and develop applications. With the importance of β-glu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01883818bec94dcf9c5b3c39f5eea3f7
https://research.manchester.ac.uk/en/publications/0cbaba0f-ec80-4906-8a92-d11264a63a7e