Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Gregory Laskaris"'
Publikováno v:
Journal of liquid chromatography & related technologies 24 (2001) 15
Journal of liquid chromatography & related technologies, 24(15), 2267-2282
Journal of liquid chromatography & related technologies, 24(15), 2267-2282
A semi-purified Taxus baccata needles extract was analysed by RP-HPLC. More than 18 taxines and cinnamates were detected by photodiode array detection and LC-MS, 10 of them being positively identified. Furthermore, 10-deacetyl baccatin III (paclitaxe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f056bbb38c19a8b38755782f1ae9b25
https://doi.org/10.1081/jlc-100105139
https://doi.org/10.1081/jlc-100105139
Publikováno v:
Plant Science. 153:97-105
Geranylgeranyl diphosphate (GGPP) synthase, an enzyme that regulates taxane biosynthesis, was purified to homogeneity from cell cultures of Taxus baccata. The molecular weight of the native protein was estimated to be 769 2 kDa, resulting from the as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d0bc36db9735635c21f818f648f1dd3b
https://doi.org/10.1016/s0168-9452(99)00263-0
https://doi.org/10.1016/s0168-9452(99)00263-0
Autor:
Georgios Theodoridis, Mina Bounkhay, Gregory Laskaris, Robert van der Heijden, Mondher El Jaziri, Robert Verpoorte
Publikováno v:
Plant Science. 147:1-8
The results presented in this paper describe for the first time the presence of an inducible enzyme in the biosynthetic pathway leading to the anticancer drug paclitaxel. Methyl jasmonate, when administered to Taxus baccata cell suspension cultures,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::61993ddec6a3a84e8fbe093b46aec9b9
https://doi.org/10.1016/s0168-9452(99)00078-3
https://doi.org/10.1016/s0168-9452(99)00078-3
Autor:
Robert van der Heijden, Georgios Theodoridis, Gregory Laskaris, Robert Verpoorte, Mondher El Jaziri, Camiel F. De Jong
Publikováno v:
Phytochemistry. 50:939-946
A Taxus baccata cell line has been characterized for growth, nutrient uptake, taxane production and for GGPP synthase activity. The enzyme is extracted by inclusion of non-ionic detergent and its activity parallels taxane production for the first thr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dce3bc124dfabbd6c16e4f4cf530a6bc
https://doi.org/10.1016/s0031-9422(98)00645-1
https://doi.org/10.1016/s0031-9422(98)00645-1
Publikováno v:
Journal of Chromatography A. 802:297-305
Reversed-phase HPLC was used for the determination of paclitaxel and related compounds in both isocratic and gradient mode, using four different HPLC columns. With the selected acetonitrile–0.05 M ammonium acetate gradient, 13 taxanes of a standard
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b080c9b49b34ebb8e9855931d6974f4b
https://doi.org/10.1016/s0021-9673(97)01174-6
https://doi.org/10.1016/s0021-9673(97)01174-6
Publikováno v:
Chromatographia. 47:25-34
Solid phase extraction (SPE) methods are studied for the sample pretreatment for HPLC analysis ofTaxus cell suspension cultures. Various types of SPE materials were tested for the extraction of both the taxane standards and samples of various origin.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6959f1d3dc3fb816b06c95563cc5d7e0
https://doi.org/10.1007/bf02466782
https://doi.org/10.1007/bf02466782