Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Gregory H. Leno"'
Autor:
Ru Gao, Karen L. Strickler, Jong S. Rim, Gregory H. Leno, Christian W. Barnes, Barbara Gawronska-Kozak, Lettie L. Harkins, Jeffery M. Gimble, Jaroslaw Staszkiewicz, Kenneth J. Eilertsen
Publikováno v:
Stem Cell Discovery. :64-71
The identification of a single, early marker for full developmental potential of induced pluripotent stem (iPS) cells has proven elusive. Recently, however, activation of the imprinted gene cluster, Dlk1-Dio3 has emerged as a viable candidate in the
Autor:
Jeffrey M. Gimble, Jaroslaw Staszkiewicz, Kenneth J. Eilertsen, Lettie L. Harkins, Christian W. Barnes, Gregory H. Leno, Karen L. Strickler, Jong S. Rim
Publikováno v:
Stem Cell Discovery. :45-57
Human somatic cells can be directly reprogrammed to induced pluripotent stem (iPS) cells by forced expression of the transcription factors Oct4, Sox2, and either Klf4 and cMyc or Nanog and Lin28, using virus-based systems. However, low reprogramming
Publikováno v:
Reproduction in Domestic Animals. 44:320-325
In several mammalian species, the configuration of germinal vesicle (GV) chromatin correlates with the developmental competence of oocytes. Yet, no study has been published on the configuration of GV chromatin in ferret, nor is it known whether a spe
Publikováno v:
Biology of Reproduction. 79:832-840
Development of an efficient cryopreservation technique for the domestic ferret is key for the long-term maintenance of valuable genetic specimens of this species and for the conservation of related endangered species. Unfortunately, current cryoprese
Autor:
Christopher S. Rogers, Juan Chen, Yulong Zhang, Ziying Yan, Xingshen Sun, Yaling Yi, Gregory H. Leno, Diana Lei, Michael J. Welsh, John F. Engelhardt, Ziyi Li
Publikováno v:
Journal of Clinical Investigation. 118:1578-1583
Somatic cell gene targeting combined with nuclear transfer cloning presents tremendous potential for the creation of new, large-animal models of human diseases. Mouse disease models often fail to reproduce human phenotypes, underscoring the need for
Publikováno v:
Theriogenology. 66:183-190
Embryo transfer (ET) to recipient females is a foundational strategy for a number of assisted reproductive technologies, including cloning by somatic cell nuclear transfer. In an attempt to develop efficient ET in domestic ferrets, factors affecting
Autor:
Cena Myers, Jeffery M. Betthauser, Paul J. Golueke, Bing Liu, Ina Hoeschele, Gregory H. Leno, Hongzhi Xu, Kenneth J. Eilertsen, Jenine C. Lacson, Martha Pfister-Genskow, Richard W. Koppang
Publikováno v:
Molecular Reproduction and Development. 73:977-986
Successful cloning by somatic cell nuclear transfer (NT) involves an oocyte-driven transition in gene expression from an inherited somatic pattern, to an embryonic form, during early development. This reprogramming of gene expression is thought to re
Autor:
Kenneth J. Eilertsen, Jeffery M. Betthauser, Paul J. Goueleke, Chiranjeet Chetia, Jenine C. Lacson, Patricia J. Fisher, Richard W. Koppang, Lynette A. Childs, Thomas Patterson, Cena Myers, Ina Hoeschele, Erik Forsberg, Ying Zheng, Gregory H. Leno, Bing Liu, Martha Pfister-Genskow, Richard M. Schultz, Gail Lange, Steven R. Watt, Xiao Yang
Publikováno v:
Biology of Reproduction. 72:546-555
Using an interwoven-loop experimental design in conjunction with highly conservative linear mixed model methodology using estimated variance components, 18 genes differentially expressed between nuclear transfer (NT)- and in vitro fertilization (IVF)
Publikováno v:
Journal of Experimental Zoology Part A: Comparative Experimental Biology. :1126-1134
The development of reconstructed embryos following nuclear transfer (NT) appears to be dependent upon a variety of factors, including cell cycle synchronization between the donor nucleus and recipient oocyte. Here we use the microtubule inhibitor, de
Autor:
David T. Brown, Susan E. Wellman, Donald B. Sittman, Siddhartha De, Zhi Hong Lu, Gregory H. Leno
Publikováno v:
Gene. 292:173-181
Multiple forms of histone H1 are found in most mammalian tissues, and diversity in their temporal and spatial expression likely corresponds to diversity in function. Here, using Xenopus egg extracts, we show that while the somatic H1s significantly i