Zobrazeno 1 - 10
of 80
pro vyhledávání: '"Gregory D. Gillispie"'
Autor:
Tory M. Schaaf, Ang Li, Benjamin D. Grant, Kurt Peterson, Samantha Yuen, Prachi Bawaskar, Evan Kleinboehl, Ji Li, David D. Thomas, Gregory D. Gillispie
Publikováno v:
Biosensors, Vol 8, Iss 4, p 99 (2018)
We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green an
Externí odkaz:
https://doaj.org/article/a15784e132c043739bbc8882f53ac9a4
Publikováno v:
SLAS Discovery. 22:250-261
We have developed a microplate reader that records a complete high-quality fluorescence emission spectrum on a well-by-well basis under true high-throughput screening (HTS) conditions. The read time for an entire 384-well plate is less than 3 min. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::761d5613c93b1f070e067df0cd0cd30c
https://doi.org/10.1177/1087057116679637
https://doi.org/10.1177/1087057116679637
Autor:
Evan Kleinboehl, Benjamin D. Grant, Ji Li, Tory M. Schaaf, Gregory D. Gillispie, Prachi Bawaskar, David D. Thomas, Samantha L. Yuen, Kurt C. Peterson, Ang Li
Publikováno v:
Biosensors
Volume 8
Issue 4
Biosensors, Vol 8, Iss 4, p 99 (2018)
Volume 8
Issue 4
Biosensors, Vol 8, Iss 4, p 99 (2018)
We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44c2341d8f0eaadef5dda87a96309984
Autor:
Benjamin D. Grant, Florentin R. Nitu, Maram M. Essawy, Robyn T. Rebbeck, David D. Thomas, Gregory D. Gillispie, Razvan L. Cornea, Donald M. Bers
Publikováno v:
Rebbeck, RT; Essawy, MM; Nitu, FR; Grant, BD; Gillispie, GD; Thomas, DD; et al.(2017). High-Throughput Screens to Discover Small-Molecule Modulators of Ryanodine Receptor Calcium Release Channels. SLAS DISCOVERY, 22(2), 176-186. doi: 10.1177/1087057116674312. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/9vz1m8kk
SLAS discovery : advancing life sciences R & D, vol 22, iss 2
SLAS discovery : advancing life sciences R & D, vol 22, iss 2
Using time-resolved fluorescence resonance energy transfer (FRET), we have developed and validated the first high-throughput screening (HTS) method to discover compounds that modulate an intracellular Ca2+ channel, the ryanodine receptor (RyR), for t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea5c2989754d7c43f4ec872e3d82b346
http://www.escholarship.org/uc/item/9vz1m8kk
http://www.escholarship.org/uc/item/9vz1m8kk
Autor:
Gregory D. Gillispie, Russell Dahl, Ji Li, David D. Thomas, Simon J. Gruber, Seth L. Robia, Kurt C. Peterson, Tory M. Schaaf, Krisztina Zsebo, Razvan L. Cornea
Publikováno v:
SLAS Discovery. 19:215-222
We have used a "two-color" SERCA (sarco/endoplasmic reticulum calcium ATPase) biosensor and a unique high-throughput fluorescence lifetime plate reader (FLT-PR) to develop a high-precision live-cell assay designed to screen for small molecules that p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92559d7461b57160966f2afcd915ad27
https://doi.org/10.1177/1087057113510740
https://doi.org/10.1177/1087057113510740
Autor:
Xiaoqiong Dong, David D. Thomas, Gregory D. Gillispie, Bradley S. Launikonis, Kenneth S. Ginsburg, Robyn T. Rebbeck, Donald M. Bers, Razvan L. Cornea, Daniel P. Singh
Publikováno v:
Biophysical Journal. 114:117a-118a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e2281cc2fc6fda8ad21b4f32a643fb3b
https://doi.org/10.1016/j.bpj.2017.11.675
https://doi.org/10.1016/j.bpj.2017.11.675
Autor:
Joseph M. Muretta, Gregory D. Gillispie, Razvan L. Cornea, Russell Dahl, David D. Thomas, Elizabeth L. Lockamy, Simon J. Gruber, Krisztina Zsebo, Jiqiu Chen, Dongzhu Jin, Tamas Bartfai
Publikováno v:
SLAS Discovery. 18:97-107
Using fluorescence resonance energy transfer (FRET), we performed a high-throughput screen (HTS) in a reconstituted membrane system, seeking compounds that reverse inhibition of sarco-/endoplasmic reticulum Ca-ATPase (SERCA) by its endogenous regulat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d36f7d650c644d002126ce2e7c39713
https://doi.org/10.1177/1087057112456878
https://doi.org/10.1177/1087057112456878
Autor:
Prachi Bawaskar, Samantha L. Yuen, David D. Thomas, Kurt C. Peterson, Gregory D. Gillispie, Tory M. Schaaf, Benjamin D. Grant, Ji Li, Joseph M. Muretta
Publikováno v:
SLAS discovery : advancing life sciences RD. 22(3)
A robust high-throughput screening (HTS) strategy has been developed to discover small-molecule effectors targeting the sarco/endoplasmic reticulum calcium ATPase (SERCA), based on a fluorescence microplate reader that records both the nanosecond dec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d8a7a21c9ce896739f3a8cd520db82f
https://pubmed.ncbi.nlm.nih.gov/27899691
https://pubmed.ncbi.nlm.nih.gov/27899691
Autor:
Tory M. Schaaf, David D. Thomas, Simon J. Gruber, Gregory D. Gillispie, Roger J. Hajjar, Prachi Bawaskar, Daniel R. Stroik
Publikováno v:
Biophysical Journal. 110(3)
We are using time-resolved FRET (TR-FRET) in living cells to probe the structural dynamics and function of sarco(endo)plasmic reticulum Ca-ATPase (SERCA) and its cardiac regulator phospholamban (PLB). Ca2+ drives muscle contraction, and relaxation re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1232fc89d725182a87d891c6ac013ca0
Autor:
Tory M. Schaaf, Ang Li, Evan Kleinboehl, Prachi Bawaskar, Gregory D. Gillispie, David D. Thomas, Benjamin D. Grant, Ji Li
Publikováno v:
Biophysical Journal. 114:171a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::56a18f9e8acd2508590f680ce6487c89
https://doi.org/10.1016/j.bpj.2017.11.955
https://doi.org/10.1016/j.bpj.2017.11.955