Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Gregory Coia"'
Publikováno v:
BioTechniques, Vol 22, Iss 5, Pp 897-904 (1997)
We have demonstrated that the expression of recombinant proteins labeled with an immunoreactive epitope can be rapidly assessed and quantitated using a modified haemagglutination inhibition assay in enzyme-linked immunosorbent assay (ELISA) trays. Th
Externí odkaz:
https://doaj.org/article/78b05ddbee4142809e4ea24455291476
Autor:
Lynne J. Waddington, Gregory Coia, Judith A. Scoble, Mioko Hirabayashi, Liliana de Campo, Calum J. Drummond, Penny A. Bean, Minoo J. Moghaddam
Publikováno v:
Biomaterials science. 2(6)
Engineering biocompatible and physiologically stable nanoscaled therapeutics and imaging agents with the ability to target tumor tissue is a key challenge for the advancement of cancer therapeutics and diagnostic imaging. Here, we present chelating a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03c7cbca26d3f8f21193ff19f5274da9
https://pubmed.ncbi.nlm.nih.gov/32481823
https://pubmed.ncbi.nlm.nih.gov/32481823
Autor:
Judith A. Scoble, Benjamin W. Muir, Nhiem Tran, Nan Li, Gregory Coia, Calum J. Drummond, Lynne J. Waddington, George O. Lovrecz, Jiali Zhai, Nigel Kirby, Xavier Mulet
Publikováno v:
Nanoscale. 7:2905-2913
Next generation drug delivery utilising nanoparticles incorporates active targeting to specific sites. In this work, we combined targeting with the inherent advantages of self-assembled lipid nanoparticles containing internal nano-structures. Epiderm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0c650a8d362545840d9ce989cff8d80
https://doi.org/10.1039/c4nr05200e
https://doi.org/10.1039/c4nr05200e
Autor:
Julie Nigro, Mariusz P. Madej, Samuel Baer, Gregory Coia, Stewart D. Nuttall, Randy Suryadinata, Rebecca M. Nisbet, Timothy E. Adams, Lisa P. T. Hong, Charlotte C. Williams
Publikováno v:
Org. Biomol. Chem.. 12:2675-2685
The Sortase A (SrtA) enzyme from Staphylococcus aureus catalyses covalent attachment of protein substrates to pentaglycine cross-bridges in the Gram positive bacterial cell wall. In vitro SrtA-mediated protein ligation is now an important protein eng
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86d69a68597d5f155047adf4f357fde2
https://doi.org/10.1039/c3ob42325e
https://doi.org/10.1039/c3ob42325e
Publikováno v:
Journal of Immunological Methods. 251:187-193
This protocol provides a simple in vivo strategy for introducing random mutations to a target DNA sequences using E. coli mutator cells. The method has been used in our laboratory for affinity maturation of proteins encoded by target DNA sequences. S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e40c9157cdaaa852cdbf450781f8edf
https://doi.org/10.1016/s0022-1759(01)00300-3
https://doi.org/10.1016/s0022-1759(01)00300-3
Autor:
Peter J. Hudson, Glenn G. Lilley, Lesley A. Pearce, Gregory Coia, Geoffrey W. Oddie, Alexander A. Kortt
Publikováno v:
IUBMB Life. 42:1179-1188
Monomeric single chain antibody (scFv) fragments lack both the avidity of the bivalent IgG, or (Fab')2 fragment, and the effector functions conferred by the Fc domain. For certain diagnostic or therapeutic applications it may be desirable to link the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2343aeb242292b7d1970e722fc3f8b6e
https://doi.org/10.1080/15216549700203651
https://doi.org/10.1080/15216549700203651
Publikováno v:
BioTechniques. 22:897-904
We have demonstrated that the expression of recombinant proteins labeled with an immunoreactive epitope can be rapidly assessed and quantitated using a modified haemagglutination inhibition assay in enzyme-linked immunosorbent assay (ELISA) trays. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b748e59457b5d7a428a1ee451f40fcb4
https://doi.org/10.2144/97225st02
https://doi.org/10.2144/97225st02
Publikováno v:
Immunotechnology. 1:197-209
Background : The current format of a rapid whole-blood agglutination assay for HIV relies on a bifunctional molecule which comprises a 10 Fab fragment, with specificity for the human red blood cell surface marker (glycophorin A), chemically conjugate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce07ac9496be0ecb9e183ad9905ebb75
https://doi.org/10.1016/1380-2933(95)00021-6
https://doi.org/10.1016/1380-2933(95)00021-6
Autor:
Timothy E. Adams, Nick Bartone, Stewart D. Nuttall, Joanne M. Caine, Rebecca M. Nisbet, Olan Dolezal, Charlotte C. Williams, Rebecca M. Attwood, Mariusz P. Madej, Gregory Coia, Lesley A. Pearce
Publikováno v:
Biotechnology and bioengineering. 109(6)
Sortase-mediated protein ligation is a biological covalent conjugation system developed from the enzymatic cell wall display mechanism found in Staphylococcus aureus. This three-component system requires: (i) purified Sortase A (SrtA) enzyme; (ii) a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2352ec83a66067bc5be43bd188ed7b9
https://pubmed.ncbi.nlm.nih.gov/22170409
https://pubmed.ncbi.nlm.nih.gov/22170409
