Zobrazeno 1 - 10 of 13 pro vyhledávání: '"Gregor Sagner"'
1
Synthesis of 3′-Sugar- and Base-Modified Nucleotides and Their Application as Potent Chain Terminators in DNA Sequencing
Autor: Ulrich Koert, Sven Klingel, Karen Stolze, Gregor Sagner, Regina Wartbichler, Joachim W. Engels
Publikováno v: Helvetica Chimica Acta. 82:1311-1323
Two 3′-modified and three base-modified ddNTPs were synthesized and tested with several DNA polymerases for incorporation activity. Starting from 3′-azido-3′-deoxythymidine (AZT; 1), we were able to produce 3′-deoxy-3′-isocyanato-thymidine
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3dfa1295b90ffc94ec43380aa147dfb5
https://doi.org/10.1002/(sici)1522-2675(19990908)82:9<1311::aid-hlca1311>3.0.co
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2
Use of platinum coproporphyrin and delayed luminescence imaging to extend the number of targets FISH karyotyping
Autor: Hans J. Tanke, Richard R. De Haas, Michael Ganser, Rob P.M. van Gijlswijk, Gregor Sagner
Publikováno v: Cytometry. 33:453-459
Combinatorial use of fluorophores in multicolor fluorescence in situ hybridization (FISH) allows for the recognition of all human chromosomes. Here we introduce the concept of the use of delayed luminescence labels such as phosphorescent platinum cop
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6062a04bdbd3f959dc46b98236e23cbe
https://doi.org/10.1002/(sici)1097-0320(19981201)33:4<453::aid-cyto9>3.0.co
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3
ChemInform Abstract: Synthesis of 3′-Sugar- and Base-Modified Nucleotides and Their Application as Potent Chain Terminators in DNA Sequencing
Autor: Sven Klingel, Joachim W. Engels, Karen Stolze, Ulrich Koert, Gregor Sagner, Regina Wartbichler
Publikováno v: ChemInform. 31
Two 3′-modified and three base-modified ddNTPs were synthesized and tested with several DNA polymerases for incorporation activity. Starting from 3′-azido-3′-deoxythymidine (AZT; 1), we were able to produce 3′-deoxy-3′-isocyanato-thymidine
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::33a1d4c9c42ca9fd177aed5b02d6f06f
https://doi.org/10.1002/chin.200002231
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4
Rapid filter assay for the detection of DNA polymerase activity: direct identification of the gene for the DNA polymerase from Thermus aquaticus
Autor: Gregor Sagner, C. Kessler, Rüdiger Rüger
Publikováno v: Gene. 97:119-123
A method for rapid identification of DNA polymerase activity employing an activated DNA substrate covalently bound to nitrocellulose membranes is described. Samples containing DNA polymerase are spotted and the membranes are incubated in an appropria
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a494a4f3074ad9e96a1b6cb6ec2d0ae4
https://doi.org/10.1016/0378-1119(91)90018-7
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6
DIG DNA Sequencing with Chemiluminescent or Dye Substrates
Autor: Gregor Sagner
Publikováno v: Nonradioactive Analysis of Biomolecules ISBN: 9783540646013
DNA sequencing based on the enzymatic dideoxy sequencing technique (Sanger et al., 1977) mainly employs two different procedures. Large-scale sequencing projects are often performed with automated sequencers using fluorescent detection (Ansorge et al
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3ae6cbf5dfe9b1c1e344ab0d871151a7
https://doi.org/10.1007/978-3-642-57206-7_59
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7
Multiplex dye DNA sequencing in capillary gel electrophoresis by diode laser-based time-resolved fluorescence detection
Autor: R. Müller, K. H. Drexhage, Jürgen Wolfrum, Gregor Sagner, Markus Sauer, Jutta Arden-Jacob, Andreas Schulz, Dirk P. Herten, Stefan Siebert, Michael Neumann, Ulrike Lieberwirth, Sven Klingel
Publikováno v: Analytical chemistry. 70(22)
A new one-lane, four-dye DNA sequencing method was developed which is based on time-resolved detection and identification of fluorescently labeled primers. For fluorescent labels, we used two newly synthesized rhodamine derivatives (MR200-1, JA169),
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f5a69cd92dda998c839ca84569c4b6c
https://pubmed.ncbi.nlm.nih.gov/9844573
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8
Purification and properties of the 5'-3' exonuclease D10A mutant of DNA polymerase I from Streptococcus pneumoniae: a new tool for DNA sequencing
Autor: Gregor Sagner, Paloma López, Mónica Amblar
Publikováno v: Journal of biotechnology. 63(1)
A D10A mutation was introduced at the 5'-3' exonuclease domain of Streptococcus pneumoniae DNA polymerase I by site directed mutagenesis of the polA gene. Introduction of the mutation resulted in a drastic decrease of the 5'-3' exonucleolytic activit
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6501e30cf455fb4cdde7c2aff597cd08
https://pubmed.ncbi.nlm.nih.gov/9764480
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9
The Digoxigenin: Anti-Digoxigenin (DIG) System
Autor: Gregor Sagner, Christoph Kessler, Josef Burg, Rüdiger Rüger, Wolfgang Hösel, G.G. Schmitz, Rudolf Seibl, Klaus Mühlegger, Thomas Walter, Anton Haselbeck, Hans-Joachim Höltke
Publikováno v: Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
The digoxigenin:anti-dioxigenin (DIG) indicator system is based on the specific interaction between the cardenolide steroid DIG, a chemically derived aglycon of digoxin and lanatoside C (see figure), and a high-affinity, DIG-specific antibody (Kessle
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::367c593f3ebb9cc9f3f6213cfe32766e
https://doi.org/10.1007/978-3-662-00144-8_3
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10
Blot Formats: Nucleic Acids
Autor: Wolfgang Schumann, Irena Bronstein, Chris S. Martin, Cortina Kaletta, Thomas Walter, Jörg T. Epplen, Christoph Kessler, Ulrich Zuber, Barbara Rüger, Thomas M. Pohl, Gregor Sagner, Judith Máthé
Publikováno v: Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
Binding between analyte and modified probe, resulting in stable hybrid molecules, is most prominently mediated by specific interaction between complementary purine and pyrimidine bases forming A:T and G:C base pairs.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e7006b7ab6b93c0f8ad4efc61aa4fe1e
https://doi.org/10.1007/978-3-662-00144-8_19
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