Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Greg Kilby"'
Analytical band centrifugation for the separation and quantification of empty and full AAV particles
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss , Pp 585-591 (2021)
Analytical band centrifugation (ABC) was first developed for the separation of macromolecules in centrifugation cells ~60 years ago. Since its development, ABC has been predominantly utilized to study macromolecular interactions or chemical reactions
Externí odkaz:
https://doaj.org/article/6a305dc9270b4dddb2acb9c44e4db46c
Autor:
Shu-Min Zhang, Matthew Roberts, Marisa Jones, Jacob Zeitler, Greg Kilby, Aston Liu, John R White
Publikováno v:
BioTechniques, Vol 68, Iss 6, Pp 353-358 (2020)
Polyethyleneimine (PEI) is a flocculent that is widely used in the downstream purification of monoclonal antibodies. It is an in-process residual that is carried through the drug purification process and strongly inhibits residual DNA quantitation by
Externí odkaz:
https://doaj.org/article/9ab2508e54994f6785b529f2abe5e12d
Autor:
Mingyan Cao, Yang Jiao, Conner Parthemore, Samuel Korman, Jiao Ma, Alan Hunter, Greg Kilby, Xiaoyu Chen
Publikováno v:
mAbs, Vol 13, Iss 1 (2021)
Fragmentation is a well-characterized degradation pathway of therapeutic antibodies and is usually monitored by capillary electrophoresis–sodium dodecyl sulfate (CE-SDS). Although fragments due to cleavage in CH2 domains linked by intrachain disulf
Externí odkaz:
https://doaj.org/article/856756af5dca4848857256f0e2f60f64
Autor:
Niluka De Mel, Weimin Chen, Greg Kilby, Dengfeng Liu, Conner Parthemore, Jihong Wang, Yang Jiao, Mingyan Cao, Shihua Lin, Xiaoyu Chen
Publikováno v:
Journal of Pharmaceutical Sciences. 111:335-344
N-terminal glutamate (E) cyclization to form pyroglutamate (pE) generates charge heterogeneities for mAbs and proteins. Thus far, pE formation rate in lyophilized formulation as compared to in liquid formulation has not been reported. Impact of pE on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::468749accdc115b5fa791181d256b41f
https://doi.org/10.1016/j.xphs.2021.09.006
https://doi.org/10.1016/j.xphs.2021.09.006
Analytical band centrifugation for the separation and quantification of empty and full AAV particles
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss, Pp 585-591 (2021)
Molecular Therapy. Methods & Clinical Development
Molecular Therapy. Methods & Clinical Development
Analytical band centrifugation (ABC) was first developed for the separation of macromolecules in centrifugation cells ~60 years ago. Since its development, ABC has been predominantly utilized to study macromolecular interactions or chemical reactions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16875933a9ea47410ed8ac51dcaaf19d
http://www.sciencedirect.com/science/article/pii/S2329050121000747
http://www.sciencedirect.com/science/article/pii/S2329050121000747
Autor:
Ben Niu, Michael Martinelli, Niluka de Mel, Eric Meinke, Weiguo Zhai, Greg Kilby, Wei Xu, Xiaoyu Chen, Chunlei Wang
Publikováno v:
Journal of chromatography. A. 1663
Therapeutic monoclonal antibodies (mAbs) are inherently heterogeneous and hence generally studied and controlled by an array of orthogonal separation methods. During drug candidate development, fractionation by HPLC is regularly employed to assist pe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::081a981eac2ee5c8e19ec2d945bdd680
https://pubmed.ncbi.nlm.nih.gov/34968959
https://pubmed.ncbi.nlm.nih.gov/34968959
Autor:
Greg Kilby, Xiaoyu Chen, Matthew Aspelund, Conner Parthemore, Alan K. Hunter, Yang Jiao, Mingyan Cao, Samuel Korman
Publikováno v:
Journal of pharmaceutical sciences. 110(8)
Site-specific cysteine engineering, along with other genetic mutations, is broadly implemented in bispecific antibodies (bsAb). Thus far, homodimer, half hole antibody, one-light chain mispaired and light chain swapped variants have been reported as
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bde2697f1c4b42790f70f0bbcfece77
https://pubmed.ncbi.nlm.nih.gov/33894207
https://pubmed.ncbi.nlm.nih.gov/33894207
Autor:
Greg Kilby, Yang Jiao, Xiaoyu Chen, Mingyan Cao, Conner Parthemore, Alan K. Hunter, Jiao Ma, Samuel Korman
Publikováno v:
mAbs
article-version (VoR) Version of Record
article-version (VoR) Version of Record
Fragmentation is a well-characterized degradation pathway of therapeutic antibodies and is usually monitored by capillary electrophoresis–sodium dodecyl sulfate (CE-SDS). Although fragments due to cleavage in CH2 domains linked by intrachain disulf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09a890c5b5911bba601a32035fe87b62
https://doi.org/10.1080/19420862.2021.1981806
https://doi.org/10.1080/19420862.2021.1981806
Autor:
Jiao Ma, Greg Kilby
Publikováno v:
Journal of proteome research. 19(8)
There is a growing industry and regulatory need to detect host cell protein (HCP) impurities in the production of protein biopharmaceuticals, as certain HCPs can impact product stability, safety, and efficacy, even at low levels. In some cases, regul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::187837f872e74616d753900438b84668
https://pubmed.ncbi.nlm.nih.gov/32449362
https://pubmed.ncbi.nlm.nih.gov/32449362