Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Greg Gillispie"'
Autor:
Tory M. Schaaf, Samantha L. Yuen, David D. Thomas, Greg Gillispie, Prachi Bawaskar, Kurt C. Peterson, Benjamin D. Grant, Ji Li
Publikováno v:
Biophysical Journal. 110:373a
We have developed a high-throughput Spectral Unmixing Plate Reader (SUPR) which can acquire a fluorescence emission spectrum across 384 wells, with short (< 4 min) read time for the entire plate. The entire spectrum is recorded at once with a compact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30dcfe33181e4da51b010f86b0cde0f9
https://doi.org/10.1016/j.bpj.2015.11.2011
https://doi.org/10.1016/j.bpj.2015.11.2011
Autor:
Katie Miller, Jacob W. Gauer, R. Bryan Sutton, Anne Hinderliter, Greg Gillispie, Sarah C. Kempka
Publikováno v:
Biophysical Journal. 100(3)
Synaptotagmin I is a calcium ion sensor involved in neurotransmitter release. When a neuron is stimulated by an action potential, an influx of calcium ion occurs causing the synaptic vesicle to fuse with the membrane, releasing the neurotransmitter i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af6d0394bada06f380366fd24afdd559
Autor:
Greg Gillispie, Karl J. Petersen, Kurt C. Peterson, Ji Li, David D. Thomas, David A. Bernlohr, Simon J. Gruber, Cornea Razvan, Tory M. Schaaf, Rocio Foncea
Publikováno v:
Biophysical Journal. 108:346a
We have developed intramolecular FRET sensors capable of detecting cytoplasmic headpiece movements of human SERCA (sarco/endo-plasmic reticulum calcium ATPase) in live-cell assays, including a non-muscle SERCA2b isoform to be used in drug discovery f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a45d963928c02d0001711566b6920133
https://doi.org/10.1016/j.bpj.2014.11.1894
https://doi.org/10.1016/j.bpj.2014.11.1894
Publikováno v:
Biophysical Journal. 100(3):510a-511a
Thermodynamic parameters capture the overall contribution to a system's energetics. In the case of binding proteins, such as Synaptotagmin I, ascertaining the overall magnitude of the interactions within the protein is the first step toward addressin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::676adc9e49dba2acd30a1a86f81e1784
Autor:
Ewa Prochniewicz, Greg Gillispie, David J. Kast, Igor V. Negrashov, Joseph M. Muretta, David D. Thomas, Yuri E. Nesmelov, Roman V. Agafonov, Piyali Guhathakurta
Publikováno v:
Biophysical Journal. 98:585a-586a
We have developed a high-throughput time-resolved fluorescence spectrometer capable of recording a high-resolution time-domain (sub-nanosecond) fluorescence decay, with high S/N every 100 μs. Coupled with a conventional stopped-flow rapid mixer, thi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5858a58c5abf45fac58a5d29291f9a1f
https://doi.org/10.1016/j.bpj.2009.12.3182
https://doi.org/10.1016/j.bpj.2009.12.3182
Autor:
Greg Gillispie, Anne Hinderliter, R. Bryan Sutton, Kristofer J. Knutson, Jacob W. Gauer, Jesse Murphy
Publikováno v:
Biophysical Journal. 98:271a
Thermodynamic parameters capture the averaged contribution to a system's energetics. In the case of binding proteins, such as Synaptotagmin I, the first step toward addressing how and where the energy is distributed within that protein is to ascertai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc73b3d3e617782418183d84c12e05f
https://doi.org/10.1016/j.bpj.2009.12.1479
https://doi.org/10.1016/j.bpj.2009.12.1479
Autor:
Anne Hinderliter, Candace Lange, Kerry L. Fuson, R. Bryan Sutton, Miguel Montes, Kristofer J. Knutson, Greg Gillispie, Jacob W. Gauer, Jesse Murphy
Publikováno v:
Biophysical Journal. 96:332a
Thermodynamic parameters capture the averaged contribution to a system's energetics. In the case of binding proteins, such as synaptotagmin I, the first step toward addressing how and where the energy is distributed within that protein is to ascertai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b09eccfc3dcaf428ae7c7f5b6f10030e
https://doi.org/10.1016/j.bpj.2008.12.1675
https://doi.org/10.1016/j.bpj.2008.12.1675