Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Grant D Schauer"'
Autor:
Lance D Langston, Ryan Mayle, Grant D Schauer, Olga Yurieva, Daniel Zhang, Nina Y Yao, Roxana E Georgescu, Mike E O'Donnell
Publikováno v:
eLife, Vol 6 (2017)
Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion in which the helicase encircles the tracking strand only and excludes the other strand from the ring. This mode of translocation allows helicases to bypas
Externí odkaz:
https://doaj.org/article/2e2204222d1346898e6fba819de74212
Autor:
Roxana E Georgescu, Grant D Schauer, Nina Y Yao, Lance D Langston, Olga Yurieva, Dan Zhang, Jeff Finkelstein, Mike E O'Donnell
Publikováno v:
eLife, Vol 4 (2015)
We have reconstituted a eukaryotic leading/lagging strand replisome comprising 31 distinct polypeptides. This study identifies a process unprecedented in bacterial replisomes. While bacteria and phage simply recruit polymerases to the fork, we find t
Externí odkaz:
https://doaj.org/article/82749394aac14281ad3f71f0c1367891
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
DNA polymerase epsilon (Pol ε) is responsible for leading strand synthesis during DNA replication. Here the authors use Cryo-EM to describe the architecture of the Pol ε holoenzyme and to provide an atomic model for the leading strand replisome.
Externí odkaz:
https://doaj.org/article/bf5369f5dc0e4ac5973ac7d20dc7daaf
Publikováno v:
Methods in enzymology. 673
R-loop proteins present a stable and robust blockade to the progression of a DNA replication fork during S-phase. The consequences of this block can include mutagenesis and other irreversible chromosomal catastrophes, causing genomic instability and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::87ac1be6fdc57793509916243d6fa557
https://pubmed.ncbi.nlm.nih.gov/35965008
https://pubmed.ncbi.nlm.nih.gov/35965008
Autor:
Antoine M. van Oijen, Jacob S. Lewis, Stefan H. Mueller, Grant D Schauer, Lisanne M. Spenkelink, Mike O'Donnell, Olga Yurieva
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance The replisome machine that duplicates the DNA genome encounters a variety of blocks to replication, such as DNA bound proteins, R-loops, and DNA lesions. Studies in bacterial systems demonstrate that replisome advance through blocks is f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cfb793876254c79256aef992d42f822
http://europepmc.org/articles/PMC7720201
http://europepmc.org/articles/PMC7720201
Publikováno v:
Helicase Enzymes Part B ISBN: 9780323997737
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fcc8b402830059f7dfebb975cf78abb4
https://doi.org/10.1016/bs.mie.2022.03.012
https://doi.org/10.1016/bs.mie.2022.03.012
Publikováno v:
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
The eukaryotic leading strand DNA polymerase (Pol) ε contains 4 subunits, Pol2, Dpb2, Dpb3 and Dpb4. Pol2 is a fusion of two B-family Pols; the N-terminal Pol module is catalytic and the C-terminal Pol module is non-catalytic. Despite extensive effo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20a55e83508e3778208c7f72c540fb24
https://pubmed.ncbi.nlm.nih.gov/32572031
https://pubmed.ncbi.nlm.nih.gov/32572031
Autor:
Flynn R. Hill, Grant D. Schauer, Roxanna E. Georgescu, Mike O'Donnell, Jacob S. Lewis, Antoine M. van Oijen, Lisanne M. Spenkelink
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 114(40), 10630-10635. NATL ACAD SCIENCES
The replisome, the multiprotein system responsible for genome duplication, is a highly dynamic complex displaying a large number of different enzyme activities. Recently, the Saccharomyces cerevisiae minimal replication reaction has been successfully
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::767ce1f9323fd75b51d71cc04bd4a76e
https://doi.org/10.1073/pnas.1711291114
https://doi.org/10.1073/pnas.1711291114
Autor:
Gurleen Kaur, Callum Kay, Olga Yurieva, Stefan H. Mueller, Antoine M. van Oijen, Lisanne M. Spenkelink, Claire Maher, Varsha Natarajan, Jacob S. Lewis, Grant D. Schauer, Mike O'Donnell
SummaryStructural and biochemical studies have revealed the basic principles of how the replisome duplicates genomic DNA, but little is known about its dynamics during DNA replication. We reconstitute the 34 proteins needed to form the S. cerevisiae
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::994c183a2548246421f663199e76c5db
https://europepmc.org/articles/PMC6943181/
https://europepmc.org/articles/PMC6943181/
Publikováno v:
Cell
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds) DNA at origins, yet must transition to single-stranded (ss) DNA for helicase action. CMG must also handle repair 18 intermediates such as reversed forks that lack ss
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9df0273f6f5b92bc7fdb95eea3702a1
https://europepmc.org/articles/PMC6705614/
https://europepmc.org/articles/PMC6705614/