Fast Protein Modification in the Nanomolar Concentration Range Using an Oxalyl Amide as Latent Thioester**.

Autor: Snella, Benoît¹ (AUTHOR), Grain, Benjamin¹ (AUTHOR), Vicogne, Jérôme¹ (AUTHOR), Capet, Frédéric² (AUTHOR), Wiltschi, Birgit³ (AUTHOR), Melnyk, Oleg¹ (AUTHOR) oleg.melnyk@ibl.cnrs.fr, Agouridas, Vangelis^1,4 (AUTHOR) vangelis.agouridas@ibl.cnrs.fr

Publikováno v: Angewandte Chemie. 7/18/2022, Vol. 134 Issue 29, p1-6. 6p.

Fast-SEA ligation: a chemoselective NCL-inspired ligation reaction effective in the nanomolar concentration range

Autor: Grain, Benjamin, Snella, Benoît, Vicogne, Jérôme, Capet, Frédéric, Wiltschi, Birgit, Melnyk, Oleg, Agouridas, Vangelis

Publikováno v: GFPP 22 / BPGM 5 Joint Meeting of the French and Belgian Peptides and Proteins Societies
GFPP 22 / BPGM 5 Joint Meeting of the French and Belgian Peptides and Proteins Societies, May 2022, Port-Leucate, France., 2022

International audience; We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::b64654c771ed283b1514f3fefb00c68c
https://hal.science/hal-03695066