An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata

Autor: Samira R. Aili, Axel Touchard, Regan Hayward, Samuel D. Robinson, Sandy S. Pineda, Hadrien Lalagüe, Mrinalini, Irina Vetter, Eivind A. B. Undheim, R. Manjunatha Kini, Pierre Escoubas, Matthew P. Padula, Garry S. A. Myers, Graham M. Nicholson

Publikováno v: Toxins, Vol 12, Iss 5, p 324 (2020)

A critical hurdle in ant venom proteomic investigations is the lack of databases to comprehensively and specifically identify the sequence and function of venom proteins and peptides. To resolve this, we used venom gland transcriptomics to generate a

Externí odkaz: https://doaj.org/article/55d7d69d39f74058b7a192f9f196b696

Label-Free, Real-Time Phospholipase-A Isoform Assay

Autor: Samira R. Aili, Matthew P. Padula, Alvaro Garcia, Axel Touchard, Christopher Murphy, Evelyne Deplazes, Graham M. Nicholson, Bruce Cornell, Upeksha Mirissa Lankage, Charles G. Cranfield

Publikováno v: ACS Biomaterials Science and Engineering
ACS Biomaterials Science and Engineering, ACS, 2020, 6 (8), pp.4714-4721. ⟨10.1021/acsbiomaterials.0c00632⟩

International audience; Phospholipase-A (PLA) enzymes catalyze the hydrolysis of ester bonds in select glycerophospholipids. Sensors for rapidly measuring the PLA activity in biological samples have relevance in the study of venom compositions and in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc4c9114be61f3c0b971949ec00af834
https://doi.org/10.1021/acsbiomaterials.0c00632

Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins

Autor: Samira R. Aili, Elsa Bonnafé, Benjamin Lefranc, Graham M. Nicholson, Axel Touchard, Valentine Barassé, Mrinalini, Laurent Coquet, Alain Dejean, R. Manjunatha Kini, Thierry Jouenne, Pierre Escoubas, Michel Treilhou, Christophe Klopp, Nathan Téné, Jérôme Leprince

Publikováno v: Journal of Proteome Research
Journal of Proteome Research, American Chemical Society, 2020, 19 (4), pp.1800-1811. ⟨10.1021/acs.jproteome.0c00048⟩

International audience; Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identifi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c37dd64b0396edc34445db921cd7aca8
https://doi.org/10.1021/acs.jproteome.0c00048

Evaluation of Chemical Strategies for Improving the Stability and Oral Toxicity of Insecticidal Peptides

Autor: Volker Herzig, Aline Dantas de Araujo, Kathryn P. Greenwood, Yanni K.-Y. Chin, Monique J. Windley, Youmie Chong, Markus Muttenthaler, Mehdi Mobli, Neil Audsley, Graham M. Nicholson, Paul F. Alewood, Glenn F. King

Publikováno v: Biomedicines, Vol 6, Iss 3, p 90 (2018)

Spider venoms are a rich source of insecticidal peptide toxins. Their development as bioinsecticides has, however, been hampered due to concerns about potential lack of stability and oral bioactivity. We therefore systematically evaluated several syn

Externí odkaz: https://doaj.org/article/376fa61b97e843a3b1e8372ffc8952b0

An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata

Autor: Samuel D. Robinson, Pierre Escoubas, Graham M. Nicholson, Regan J. Hayward, Sandy S. Pineda, Samira R. Aili, Axel Touchard, Garry S. A. Myers, Matthew P. Padula, R. Manjunatha Kini, Hadrien Lalagüe, Mrinalini, Eivind A. B. Undheim, Irina Vetter

Publikováno v: Toxins
Toxins, MDPI, 2020, 12 (5), pp.324. ⟨10.3390/toxins12050324⟩
324-?
Volume 12
Issue 5
Toxins, Vol 12, Iss 324, p 324 (2020)

A critical hurdle in ant venom proteomic investigations is the lack of databases to comprehensively and specifically identify the sequence and function of venom proteins and peptides. To resolve this, we used venom gland transcriptomics to generate a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1cee10d1965909b99fa66ba9402910b
https://hal.inrae.fr/hal-03196489