Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Grace N, Hibshman"'
Autor:
Grace N. Hibshman, Jack P. K. Bravo, Matthew M. Hooper, Tyler L. Dangerfield, Hongshan Zhang, Ilya J. Finkelstein, Kenneth A. Johnson, David W. Taylor
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract CRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the number of editable genes. Recently developed Cas9 variants have
Externí odkaz:
https://doaj.org/article/bcc8918303d84f72875bedfc0ceaf526
Autor:
Jack P. K. Bravo, Mu-Sen Liu, Grace N. Hibshman, Tyler L. Dangerfield, Kyungseok Jung, Ryan S. McCool, Kenneth A. Johnson, David W. Taylor
Publikováno v:
Nature. 603:343-347
CRISPR–Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage1–4, and the underlying mechanisms by which Cas9 recognizes mismatches are poorly understood5–7. Although Cas9 variants with greater discrimination against
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::984fc4cf77a551b3116aa60d4788ab46
https://doi.org/10.1038/s41586-022-04470-1
https://doi.org/10.1038/s41586-022-04470-1
Autor:
Roisin E. O’Brien, Jack P.K. Bravo, Delisa Ramos, Grace N. Hibshman, Jacquelyn T. Wright, David W. Taylor
Cascades are RNA-guided multi-subunit CRISPR-Cas surveillances complexes that target foreign nucleic acids for destruction. Here, we present a 2.9-Å resolution cryo-electron (cryo-EM) structure of the D. vulgaris type I-C Cascade bound to a double-s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::16abd116161089dd5f7ab7a78667c045
https://doi.org/10.1101/2022.06.15.496202
https://doi.org/10.1101/2022.06.15.496202
Autor:
Roisin E. O’Brien, Jack P.K. Bravo, Delisa Ramos, Grace N. Hibshman, Jacquelyn T. Wright, David W. Taylor
Publikováno v:
Mol Cell
Type I CRISPR-Cas systems employ multi-subunit Cascade effector complexes to target foreign nucleic acids for destruction. Here, we present structures of D. vulgaris type I-C Cascade at various stages of double-stranded (ds)DNA target capture, reveal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9368ed7e479262572b800fd9b272ef94
https://doi.org/10.1016/j.molcel.2023.01.024
https://doi.org/10.1016/j.molcel.2023.01.024
Publikováno v:
Current Opinion in Biotechnology. 78:102839
Clustered regularly interspaced short palindromic repeats - CRISPR-associated protein (CRISPR-Cas) systems are a critical component of the bacterial adaptive immune response. Since the discovery that they can be reengineered as programmable RNA-guide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0e46a9c9128790d62025d84ca99c500
https://doi.org/10.1016/j.copbio.2022.102839
https://doi.org/10.1016/j.copbio.2022.102839
Autor:
Jack P K, Bravo, Mu-Sen, Liu, Grace N, Hibshman, Tyler L, Dangerfield, Kyungseok, Jung, Ryan S, McCool, Kenneth A, Johnson, David W, Taylor
Publikováno v:
Nature. 603(7900)
CRISPR-Cas9 as a programmable genome editing tool is hindered by off-target DNA cleavage
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5140ff34a4dfaefea14087abd0a9ece0
https://pubmed.ncbi.nlm.nih.gov/35927241
https://pubmed.ncbi.nlm.nih.gov/35927241
Autor:
Jack P. K. Bravo, Mu-Sen Liu, Grace N. Hibshman, Tyler L. Dangerfield, Kyungseok Jung, Ryan S. McCool, Kenneth A. Johnson, David W. Taylor
Publikováno v:
Nature. 604:E10-E10
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::36da55480d164556ef9ee166fb4f09d5
https://doi.org/10.1038/s41586-022-04655-8
https://doi.org/10.1038/s41586-022-04655-8