Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Grace E. Vezeau"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-13 (2023)
Abstract Cell-free genetically encoded biosensors have been developed to detect small molecules and nucleic acids, but they have yet to be reliably engineered to detect proteins. Here we develop an automated platform to convert protein-binding RNA ap
Externí odkaz:
https://doaj.org/article/e8de8022aec34bdd90f985ed2f9434ca
Tuning Cell-Free Composition Controls the Time Delay, Dynamics, and Productivity of TX-TL Expression
Autor:
Howard M. Salis, Grace E. Vezeau
Publikováno v:
ACS Synthetic Biology. 10:2508-2519
The composition of cell-free expression systems (TX-TL) is adjusted by adding macromolecular crowding agents and salts. However, the effects of these cosolutes on the dynamics of individual gene expression processes have not been quantified. Here, we
Autor:
Ayaan Hossain, Sean M. Halper, Grace E. Vezeau, Daniel P. Cetnar, Phillip R. Clauer, Alexander C. Reis, Howard M. Salis
Publikováno v:
Nature Biotechnology. 37:1294-1301
Engineering cellular phenotypes often requires the regulation of many genes. When using CRISPR interference, coexpressing many single-guide RNAs (sgRNAs) triggers genetic instability and phenotype loss, due to the presence of repetitive DNA sequences
Autor:
Grace E. Vezeau, Neil Taylor, Matthew Csordas, Ivan Manzano, Andrew L. Zydney, Howard M. Salis
Publikováno v:
Biotechnology progressREFERENCES. 37(2)
The discovery of CRISPR-Cas9 has revolutionized molecular biology, greatly accelerating the introduction of genetic modifications into organisms and facilitating the development of novel therapeutics and diagnostics. For many applications, guide RNA
Sporulated cells have potential as time-delayed expression chassis of proteins for applications such as ‘on-demand’ biologics production, whole cell biosensors, or oral vaccines. However, the desired attributes of high expression rates and low pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f85e5e9609e27b2de10df15741be201
https://doi.org/10.1101/592659
https://doi.org/10.1101/592659
Publikováno v:
Separation and Purification Technology. 237:116372
Successful commercialization of RNA therapeutics will require the development of appropriate manufacturing and purification processes suitable for the production of large quantities of high quality and high purity RNA with well-defined tertiary struc
Publikováno v:
Environmental sciencetechnology. 49(20)
Emiliania huxleyi, a ubiquitous marine algae, was cultured under replete and Cu-limiting conditions to investigate Cu uptake strategies involving thiols and associated redox reactions; comparisons to a model diatom, Thalassiosira pseudonana, were als