Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Grace A. McLaughlin"'
Autor:
John M. Crutchley, Jay M. Newby, Amy S. Gladfelter, Grace A. McLaughlin, Erin M Langdon, Liam J. Holt, M. Gregory Forest
Publikováno v:
Molecular Biology of the Cell
The spatial structure and physical properties of the cytosol are not well understood. Measurements of the material state of the cytosol are challenging due to its spatial and temporal heterogeneity. Recent development of genetically encoded multimeri
Autor:
Christine A Roden, Yifan Dai, Catherine A Giannetti, Ian Seim, Myungwoon Lee, Rachel Sealfon, Grace A McLaughlin, Mark A Boerneke, Christiane Iserman, Samuel A Wey, Joanne L Ekena, Olga G Troyanskaya, Kevin M Weeks, Lingchong You, Ashutosh Chilkoti, Amy S Gladfelter
Publikováno v:
bioRxiv
Nucleocapsid protein (N-protein) is required for multiple steps in betacoronaviruses replication. SARS-CoV-2-N-protein condenses with specific viral RNAs at particular temperatures making it a powerful model for deciphering RNA sequence specificity i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::168775dbfec2208cfe14485d85cd3550
https://doi.org/10.1101/2021.06.14.448452
https://doi.org/10.1101/2021.06.14.448452
Autor:
Marcus Roper, Erin M Langdon, Amy S. Gladfelter, Benjamin M. Stormo, Therese M. Gerbich, Grace A. McLaughlin, Bin Yu
RNA-binding proteins are frequently seen to be capable of auto-regulation by binding their own transcripts. In this work, we show that in the multinucleated fungusAshbya gossypii, the phase-separating RNA-binding protein Whi3 binds and regulates its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d988657b86350ce0212d1ec0b9b6da8c
https://doi.org/10.1101/2021.04.25.441362
https://doi.org/10.1101/2021.04.25.441362
Autor:
Jessica Liu, Grace A. McLaughlin, Benjamin L. Woods, Kevin S. Cannon, Amy S. Gladfelter, Ian Seim
Septin filaments build structures such as rings, lattices and gauzes that serve as platforms for localizing signaling and organizing cell membranes. How cells control the geometry of septin assemblies in poorly understood. We show here that septins a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46ebba8121eafc98cbdbc2cf1a6a4160
https://doi.org/10.1101/2021.03.22.436414
https://doi.org/10.1101/2021.03.22.436414
Autor:
Ethan J. Fritch, Christine A. Roden, Manolis Kellis, Amy S. Gladfelter, Mark A. Boerneke, Ralph S. Baric, Timothy P. Sheahan, Kevin M. Weeks, Grace A. McLaughlin, Chandra L. Theesfeld, Joanne Ekena, Yixuan J. Hou, Christiane Iserman, Chase A. Weidmann, Rachel Sealfon, Olga G. Troyanskaya, Irwin Jungreis
Publikováno v:
Molecular Cell
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic elements under physiological buffer conditions and condensation is enhanced a
Autor:
Chandra L. Theesfeld, Avinash Boppana, Amy S. Gladfelter, Christine A. Roden, Mark A. Boerneke, Kevin M. Weeks, Rachel Sealfon, Timothy P. Sheahan, Yixuan J. Hou, Grace A. McLaughlin, Ralph S. Baric, Olga G. Troyanskaya, Christopher Y. Park, Ethan J. Fritch, Irwin Jungreis, Christiane Iserman
Publikováno v:
bioRxiv
article-version (status) pre
article-version (number) 1
article-version (status) pre
article-version (number) 1
A mechanistic understanding of the SARS-CoV-2 viral replication cycle is essential to develop new therapies for the COVID-19 global health crisis. In this study, we show that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid pha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a1b9d24a24eef5f846edcca904aa94c
https://doi.org/10.1101/2020.06.11.147199
https://doi.org/10.1101/2020.06.11.147199
Autor:
Mark A. Boerneke, Kevin M. Weeks, Rachel Sealfon, Ethan J. Fritch, Avinash Boppana, Amy S. Gladfelter, Christine A. Roden, Grace A. McLaughlin, Irwin Jungreis, Yixuan J. Hou, Olga G. Troyanskaya, Christiane Iserman, Christopher Y. Park, Chandra L. Theesfeld, Ralph S. Baric, Timothy P. Sheahan
Publikováno v:
SSRN Electronic Journal.
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with the viral genome and propose a model of viral packaging through LLPS. N-protein condenses with specific RNA sequences in the first 100
Autor:
Grace A. McLaughlin, John M. Crutchley, Erin M Langdon, Amy S. Gladfelter, Liam J. Holt, Jay M. Newby, Mark Gregory Forest
The spatial structure and physical properties of the cytosol are not well understood. Measurements of the material state of the cytosol are challenging due to its spatial and temporal heterogeneity, the lack of truly passive probes, and the need for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e718837b49008cba65d22705007c867c
https://doi.org/10.1101/823286
https://doi.org/10.1101/823286
Autor:
Scott A. Gerber, Katelyn B. Cassidy, Therese M. Gerbich, David Adalsteinsson, Grace A. McLaughlin, Amy S. Gladfelter
Publikováno v:
The Journal of Cell Biology
Cells must control the composition and location of biomolecular condensates. This study shows that phosphorylation of the RNA-binding protein Whi3 is used to regulate functionally distinct condensates important for cell polarity and nuclear division
Autor:
Grace A. McLaughlin, Amy S. Gladfelter, Jay M. Newby, Jia Zhao, M. Gregory Forest, Kelsey Gasior
Publikováno v:
Mathematics and Statistics Faculty Publications
An emerging mechanism for intracellular organization is liquid-liquid phase separation (LLPS). Found in both the nucleus and the cytoplasm, liquid-like droplets condense to create compartments that are thought to promote and inhibit specific biochemi