Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Govindan Raghunathan"'
Autor:
Nagasundarapandian Soundrarajan, Sriram Sokalingam, Govindan Raghunathan, Nediljko Budisa, Hyun-Jong Paik, Tae Hyeon Yoo, Sun-Gu Lee
Publikováno v:
PLoS ONE, Vol 7, Iss 10, p e46741 (2012)
N-terminal site-specific modification of a protein has many advantages over methods targeting internal positions, but it is not easy to install reactive groups onto a protein in an N-terminal specific manner. We here report a strategy to incorporate
Externí odkaz:
https://doaj.org/article/f058328453064eb9ae2ddef4b5557d8f
Autor:
Govindan Raghunathan, Nagasundarapandian Soundrarajan, Sriram Sokalingam, Hyungdon Yun, Sun-Gu Lee
Publikováno v:
PLoS ONE, Vol 7, Iss 12, p e51510 (2012)
Diversification of protein sequence-structure space is a major concern in protein engineering. Deletion mutagenesis can generate a protein sequence-structure space different from substitution mutagenesis mediated space, but it has not been widely use
Externí odkaz:
https://doaj.org/article/abc03c980ff54994a8a7cc4938bcb915
Publikováno v:
PLoS ONE, Vol 7, Iss 7, p e40410 (2012)
Two positively charged basic amino acids, arginine and lysine, are mostly exposed to protein surface, and play important roles in protein stability by forming electrostatic interactions. In particular, the guanidinium group of arginine allows interac
Externí odkaz:
https://doaj.org/article/d58ed458687c4243bbd997f384b54da1
Publikováno v:
Journal of Industrial and Engineering Chemistry. 46:337-341
s-DL4 is a variant of green fluorescent protein (GFP), exclusively deposited in vivo into active inclusion bodies (IBs). In this study, we demonstrated that s-DL4 is a self-aggregating molecule by performing structural analysis of s-DL4 IBs and study
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d1f85de4b079a998049b7f14b3eee4b1
https://doi.org/10.1016/j.jiec.2016.11.001
https://doi.org/10.1016/j.jiec.2016.11.001
Autor:
Jin-Hoi Kim, Suhyun Park, Govindan Raghunathan, Hyuk Song, Nagasundarapandian Soundrarajan, Hye-sun Cho, Chankyu Park, Quy Le Van Chanh, Byeongyong Ahn
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
Scientific Reports
Scientific Reports
Porcine protegrin-1 (PG-1) is a broad-spectrum antimicrobial peptide (AMP) with potent antimicrobial activities. We produced recombinant PG-1 and evaluated its cytotoxicity toward various types of mammalian cell lines, including embryonic fibroblasts
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff93192384f7b2a8397cd5e3f61d15b8
https://doi.org/10.1038/s41598-019-47955-2
https://doi.org/10.1038/s41598-019-47955-2
Autor:
Govindan Raghunathan, Andreas Marx
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
DNA polymerases the key enzymes for several biotechnological applications. Obviously, nature has not evolved these enzymes to be compatible with applications in biotechnology. Thus, engineering of a natural scaffold of DNA polymerases may lead to enz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::114a86bc56a35b659312579cb44bf084
http://europepmc.org/articles/PMC6345897
http://europepmc.org/articles/PMC6345897
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 82:2812-2822
Both Type I' and Type II' β-turns have the same sense of the β-turn twist that is compatible with the β-sheet twist. They occur predominantly in two residue β-hairpins, but the occurrence of Type I' β-turns is two times higher than Type II' β-t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c675641c8506edaa1773f2eac8a4be2b
https://doi.org/10.1002/prot.24644
https://doi.org/10.1002/prot.24644
Publikováno v:
Biotechnology and Bioprocess Engineering. 18:858-867
Charged amino acids having ionizable side chains play crucial roles in maintaining the solubility and stability of a protein. These charged amino acids are mostly exposed on protein surface and participate in electrostatic interactions with neighbori
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::81191ef65f7690fdf53eebcbf0df0947
https://doi.org/10.1007/s12257-013-0309-1
https://doi.org/10.1007/s12257-013-0309-1
Autor:
Sriram Sokalingam, Nagasundarapandian Soundrarajan, Ganapathiraman Munussami, Sun-Gu Lee, Govindan Raghunathan, Bharat Madan
Publikováno v:
Biotechnology and Bioprocess Engineering. 18:342-349
Green fluorescent protein (GFP) has been used as a reporter marker in a wide range of biological and bioengineering studies. The expanded use of GFP in the field of biosensors, biochips and bio-conjugations requires the stability of GFP in organic co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bcc7997316950f2081e4ae2aebb43982
https://doi.org/10.1007/s12257-012-0579-z
https://doi.org/10.1007/s12257-012-0579-z
Publikováno v:
Biotechnology and Bioprocess Engineering. 18:18-26
Charged amino acids are mostly exposed on a protein surface, thereby forming a network of interactions with the surrounding amino acids as well as with water. In particular, positively charged arginine and lysine have different side chain geometries
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4713af70439742ac37b5419af7f82ce6
https://doi.org/10.1007/s12257-012-0516-1
https://doi.org/10.1007/s12257-012-0516-1