Zobrazeno 1 - 10
of 369
pro vyhledávání: '"Gottfried Otting"'
Autor:
Joe A. Kaczmarski, Mithun C. Mahawaththa, Akiva Feintuch, Ben E. Clifton, Luke A. Adams, Daniella Goldfarb, Gottfried Otting, Colin J. Jackson
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
Cyclohexadienyl dehydratase (CDT) evolved from a cationic amino acid binding protein ancestor without enzymatic activity (AncCDT-1) via a series of intermediates. Here, the authors combine EPR, X-ray crystallography and MD simulations to study the st
Externí odkaz:
https://doaj.org/article/c19f20f6641e4d50a18da243f7703954
Autor:
Kasuni B. Ekanayake, Mithun C. Mahawaththa, Haocheng Qianzhu, Elwy H. Abdelkader, Josemon George, Sven Ullrich, Rhys B. Murphy, Sarah E. Fry, Jason Johansen-Leete, Richard J. Payne, Christoph Nitsche, Thomas Huber, Gottfried Otting
Publikováno v:
Journal of Medicinal Chemistry. 66:5289-5304
Autor:
Emma E. Watson, Xuyu Liu, Robert E. Thompson, Jorge Ripoll-Rozada, Mike Wu, Imala Alwis, Alessandro Gori, Choy-Theng Loh, Benjamin L. Parker, Gottfried Otting, Shaun Jackson, Pedro José Barbosa Pereira, Richard J. Payne
Publikováno v:
ACS Central Science, Vol 4, Iss 4, Pp 468-476 (2018)
Externí odkaz:
https://doaj.org/article/39f6c6008a294a2faf94d657f88622d6
Autor:
Ruhu Qi, Gottfried Otting
Publikováno v:
PLoS ONE, Vol 14, Iss 1, p e0211065 (2019)
The advent of high-fidelity DNA polymerases that can be used to linearize and amplify whole plasmids by PCR opened the door to greatly simplified cloning and mutagenesis protocols. Commercially available kits work well, but often have been optimized
Externí odkaz:
https://doaj.org/article/2f13e6f3abd24ed99db92c495dff54bd
The novel eCell system maintains the activity of the entire repertoire of metabolic Escherichia coli enzymes in cell-free protein synthesis. We show that this can be harnessed to produce proteins with selectively 13C-labelled amino acids from inexpen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dba9dc31e243fbabef9e0b56f0900c7
https://mr.copernicus.org/preprints/mr-2023-3/
https://mr.copernicus.org/preprints/mr-2023-3/
Publikováno v:
ACS Sensors. 7:44-49
Publikováno v:
Organic & Biomolecular Chemistry. 20:2424-2432
19F-NMR spectra of the protein GB1 produced by cell-free protein synthesis with fluorinated amino acids substituting their canonical parent amino acids show exceptionally large chemical shift dispersions, which indicate structural conservation.
Autor:
Bim Graham, Shereen Jabar, Gottfried Otting, Ansis Maleckis, Iresha D. Herath, Colum Breen, Lydia Topping, Henry W. Orton, Stephen J. Butler, Monika Szabo
Publikováno v:
Magnetic Resonance, Vol 3, Pp 1-13 (2022)
The metallo-β-lactamase IMP-1 features a flexible loop near the active site that assumes different conformations in single crystal structures, which may assist in substrate binding and enzymatic activity. To probe the position of this loop, we label