Evaluation of the RNA-dependence of PRC2 binding to chromatin in human pluripotent stem cells.

Autor: Long Y; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA.; Howard Hughes Medical Institute, University of Colorado, Boulder, CO, USA.; Present address: Cardiovascular Research Institute, Department of Biochemistry, Weill Cornell Medicine, New York, NY, USA., Hwang T; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA.; Present address: Lieber Institute for Brain Development, Department of Neurology, Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Gooding AR; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA.; Howard Hughes Medical Institute, University of Colorado, Boulder, CO, USA., Goodrich KJ; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA.; Howard Hughes Medical Institute, University of Colorado, Boulder, CO, USA., Hanson SD; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA., Vallery TK; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA.; Present address: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA., Rinn JL; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA., Cech TR; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, USA.; Howard Hughes Medical Institute, University of Colorado, Boulder, CO, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 May 02. Date of Electronic Publication: 2024 May 02.

CST does not evict elongating telomerase but prevents initiation by ssDNA binding.

Autor: Zaug AJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA.; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80309, USA., Lim CJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA., Olson CL; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA., Carilli MT; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA., Goodrich KJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA.; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80309, USA., Wuttke DS; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA., Cech TR; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80309, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80309, USA.; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80309, USA.

Publikováno v: Nucleic acids research [Nucleic Acids Res] 2021 Nov 18; Vol. 49 (20), pp. 11653-11665.

The structure of human CST reveals a decameric assembly bound to telomeric DNA.

Autor: Lim CJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA., Barbour AT; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA., Zaug AJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA.; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303, USA., Goodrich KJ; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA.; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303, USA., McKay AE; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA., Wuttke DS; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA. thomas.cech@colorado.edu deborah.wuttke@colorado.edu., Cech TR; Department of Biochemistry, University of Colorado Boulder, Boulder, CO 80303, USA. thomas.cech@colorado.edu deborah.wuttke@colorado.edu.; BioFrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA.; Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2020 Jun 05; Vol. 368 (6495), pp. 1081-1085.

Conserved RNA-binding specificity of polycomb repressive complex 2 is achieved by dispersed amino acid patches in EZH2.

Autor: Long Y; Department of Chemistry and Biochemistry, BioFrontiers Institute, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States., Bolanos B; Worldwide Medicinal Chemistry, Worldwide Research and Development, Pfizer Inc., San Diego, United States., Gong L; Cecil H. and Ida Green Center for Reproductive Biology Sciences, Division of Basic Research, Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, United States.; Department of Biophysics, UT Southwestern Medical Center, Dallas, United States., Liu W; Worldwide Medicinal Chemistry, Worldwide Research and Development, Pfizer Inc., San Diego, United States., Goodrich KJ; Department of Chemistry and Biochemistry, BioFrontiers Institute, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States., Yang X; Cecil H. and Ida Green Center for Reproductive Biology Sciences, Division of Basic Research, Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, United States.; Department of Biophysics, UT Southwestern Medical Center, Dallas, United States., Chen S; Cecil H. and Ida Green Center for Reproductive Biology Sciences, Division of Basic Research, Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, United States.; Department of Biophysics, UT Southwestern Medical Center, Dallas, United States., Gooding AR; Department of Chemistry and Biochemistry, BioFrontiers Institute, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States., Maegley KA; Oncology Research Unit, Worldwide Research and Development, Pfizer Inc., San Diego, United States., Gajiwala KS; Worldwide Medicinal Chemistry, Worldwide Research and Development, Pfizer Inc., San Diego, United States., Brooun A; Worldwide Medicinal Chemistry, Worldwide Research and Development, Pfizer Inc., San Diego, United States., Cech TR; Department of Chemistry and Biochemistry, BioFrontiers Institute, Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, United States., Liu X; Cecil H. and Ida Green Center for Reproductive Biology Sciences, Division of Basic Research, Department of Obstetrics and Gynecology, UT Southwestern Medical Center, Dallas, United States.; Department of Biophysics, UT Southwestern Medical Center, Dallas, United States.

Publikováno v: ELife [Elife] 2017 Nov 29; Vol. 6. Date of Electronic Publication: 2017 Nov 29.

RNA Duplex Map in Living Cells Reveals Higher-Order Transcriptome Structure.

Autor: Lu Z; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA., Zhang QC; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA; MOE Key Laboratory of Bioinformatics, Beijing Advanced Innovation Center for Structural Biology, Center for Synthetic and Systems Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China., Lee B; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA., Flynn RA; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA., Smith MA; RNA Biology and Plasticity Group, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, UNSW Medicine, NSW 2052, Australia., Robinson JT; Department of Medicine and Moores Cancer Center, University of California San Diego, La Jolla, CA 92093, USA., Davidovich C; HHMI and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80303, USA; Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Victoria 3800, Australia; EMBL Australia and the ARC Centre of Excellence in Advanced Molecular Imaging, Clayton, VIC 3800, Australia., Gooding AR; HHMI and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80303, USA., Goodrich KJ; HHMI and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80303, USA., Mattick JS; RNA Biology and Plasticity Group, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia; St Vincent's Clinical School, UNSW Medicine, NSW 2052, Australia., Mesirov JP; Department of Medicine and Moores Cancer Center, University of California San Diego, La Jolla, CA 92093, USA., Cech TR; HHMI and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80303, USA., Chang HY; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA. Electronic address: howchang@stanford.edu.

Publikováno v: Cell [Cell] 2016 May 19; Vol. 165 (5), pp. 1267-1279. Date of Electronic Publication: 2016 May 12.