Characterization and application of recombinant Bovine Leukemia Virus Env protein

Autor: Lorena Tomé-Poderti, Natalia Olivero-Deibe, Federico Carrión, María Magdalena Portela, Gonzalo Obal, Gleysin Cabrera, Sergio Bianchi, Analia Lima, Andrés Addiego, Rosario Durán, Gonzalo Moratorio, Otto Pritsch

Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-16 (2024)

Abstract The Bovine Leukemia Virus (BLV) Envelope (Env) glycoprotein complex is instrumental in viral infectivity and shapes the host’s immune response. This study presents the production and characterization of a soluble furin-mutated BLV Env ecto

Externí odkaz: https://doaj.org/article/139bd125a2c14901bafd8de7398bbb7c

Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).

Autor: Agustín Correa, Sabino Pacheco, Ariel E Mechaly, Gonzalo Obal, Ghislaine Béhar, Barbara Mouratou, Pablo Oppezzo, Pedro M Alzari, Frédéric Pecorari

Publikováno v: PLoS ONE, Vol 9, Iss 5, p e97438 (2014)

Glycosidases are associated with various human diseases. The development of efficient and specific inhibitors may provide powerful tools to modulate their activity. However, achieving high selectivity is a major challenge given that glycosidases with

Externí odkaz: https://doaj.org/article/b257c58438d04cc4a4c882f220efb2f0

A human monoclonal antibody targeting a conserved pocket in the SARS-CoV-2 receptor-binding domain core

Autor: Ieva Drulyte, Wentao Li, Chunyan Wang, Berend Jan Bosch, Stuart C. Howes, Frank J. M. van Kuppeveld, Daniel L. Hurdiss, Friedrich Förster, Gonzalo Obal, Juliette Fedry

Publikováno v: bioRxiv
biorxiv

SARS-CoV-2 has caused a global outbreak of severe respiratory disease (COVID-19), leading to an unprecedented public health crisis. To date, there has been over thirty-three million diagnosed infections, and over one million deaths. No vaccine or tar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae1170456971825cf3b5eb3a0a1f2481
https://doi.org/10.1101/2020.09.30.318261

A novel, smaller scaffold for Affitins: Showcase with binders specific for EpCAM

Autor: Valentina Kalichuk, Véronique Préat, Gonzalo Obal, Frédéric Pecorari, Mike Maillasson, Federico Carrión, Barbara Mouratou, Ghislaine Béhar, Axelle Renodon-Cornière

Publikováno v: Biotechnology and Bioengineering
Biotechnology and Bioengineering, Wiley, 2017, Epub ahead of print. ⟨10.1002/bit.26463⟩
Biotechnology and Bioengineering, 2017, Epub ahead of print. ⟨10.1002/bit.26463⟩

International audience; Affitins are highly stable engineered affinity proteins, originally derived from Sac7d and Sso7d, two 7 kDa DNA-binding polypeptides from Sulfolobus genera. Their efficiency as reagents for intracellular targeting, enzyme inhi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc3faee1c79e143246d3a00a336eb44d
https://www.hal.inserm.fr/inserm-01629637/file/KalichukEq13.pdf

Regulation of signaling directionality revealed by 3D snapshots of a kinase:regulator complex in action

Autor: Marcelo A. Martí, Ariel E. Mechaly, Gonzalo Obal, J.A. Imelio, Nicole Larrieux, Alejandro Buschiazzo, Felipe Trajtenberg, Matías R. Machado

Publikováno v: eLife, Vol 5 (2016)
eLife
eLife, eLife Sciences Publication, 2016, 5, pp.e21422. ⟨10.7554/eLife.21422⟩
eLife, 2016, 5, pp.e21422. ⟨10.7554/eLife.21422⟩

Two-component systems (TCS) are protein machineries that enable cells to respond to input signals. Histidine kinases (HK) are the sensory component, transferring information toward downstream response regulators (RR). HKs transfer phosphoryl groups t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94f50326360c2b6ecffe90612b0d66f5
https://elifesciences.org/articles/21422