Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Gonzalo J. Mazzei"'
Publikováno v:
International Journal of Cancer. 47:450-454
The human leukemic cell line AML-193 was tested for its proliferative response to endogenously produced autocrine factors and to a variety of cytokines and colony-stimulating factors. Cells grown in the absence of GM-CSF incorporated tritiated thymid
Publikováno v:
Cytokine. 2:123-128
An interleukin 1 (IL 1) inhibitor is secreted into culture medium by a human promyclocytic cell line, H-161, upon stimulation with (PMA) and recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF). Since the morphological charac
Publikováno v:
Proceedings of the National Academy of Sciences. 82:3030-3034
Antisera against phospholipid/Ca2+-dependent protein kinase (protein kinase C) were raised in rabbits. Immunospecificity of the polyclonal antibodies, as determined by immunoblot and ELISA, was shown by their reactivity to the enzyme but not to other
Publikováno v:
Biochemical Journal. 213:281-288
Effects of polyamines on various protein kinases were investigated. It was found that both phospholipid-sensitive Ca2+-dependent protein kinase and myosin light-chain kinase (a calmodulin-sensitive species of Ca2+-dependent protein kinase) were inhib
Publikováno v:
Advances in Enzyme Regulation. 25:387-392
The substrate specificity determinants of protein kinase C are probed using synthetic peptides encompassing the major phosphorylation site serine 115 in bovine MBP. The results indicate that basic residues arginine 107 and 113 N-terminal to the phosp
Publikováno v:
Biochemical Pharmacology. 34:3649-3653
Antiestrogens (tamoxifen, clomiphene and nafoxidine) were found to inhibit phospholipid/ Ca2+-dependent protein kinase (PL/Ca-PK. or protein kinase C). whereas estrogens (estradiol and diethylstilbesterol) and the weakly estrogenic chlorotrianisene w
Publikováno v:
Biochemical and Biophysical Research Communications. 135:1144-1149
The ability of tumor promoting 12-O-tetradecanoylphorbol-13-acetate (TPA) to redistribute protein kinase C in human promyelocytic leukemic HL60 cells was investigated. It was found that TPA caused a rapid translocation (within 10 min) of protein kina
Autor:
Francesco A. Manzoli, Silvano Capitani, Gonzalo J. Mazzei, J.F. Kuo, Ronald Berezney, Peggy R. Girard
Publikováno v:
Biochemical and Biophysical Research Communications. 142:367-375
A doublet of immunoreactive bands has been identified in rat liver nuclei, nuclear matrix and lamina by means of a polyclonal antibody against protein kinase C. The two polypeptides show an apparent molecular weight of 77 and 74 kDa on SDS-polyacryla
Publikováno v:
Biochemical pharmacology. 33(1)
R-24571 (calmidazolium), a derivative of the antimycotic agent miconazole, inhibited phospholipid-sensitive Ca 2+ -dependent protein kinase (PL-Ca-PK), with an ic 50 (the concentration causing 50% inhibition) of 5.3 μM. It also inhibited the calmodu
Publikováno v:
Biochemical and biophysical research communications. 109(4)
Polymyxin B inhibited phospholipid-sensitive Ca2+-dependent protein kinase competitively with respect to phosphatidylserine (a phospholipid cofactor), with a Ki of 1.8 μM. It also inhibited myosin light chain kinase (a calmodulin-sensitive species o