Saccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme expressed in Pichia pastoris.

Autor: Facchinetti de Castro Girão L; Federal University of Rio de Janeiro, Institute of Chemistry, Department of Biochemistry, Enzyme Technology Laboratory, 21941-909, Rio de Janeiro, Rio de Janeiro, Brazil; Oswaldo Cruz Foundation, Oswaldo Cruz Institute, Toxinology Laboratory, 21040-900, Rio de Janeiro, Rio de Janeiro, Brazil., Gonçalves da Rocha SL; Oswaldo Cruz Foundation, Oswaldo Cruz Institute, Toxinology Laboratory, 21040-900, Rio de Janeiro, Rio de Janeiro, Brazil., Sobral RS; Federal University of Rio de Janeiro, Institute of Chemistry, Department of Biochemistry, Enzyme Technology Laboratory, 21941-909, Rio de Janeiro, Rio de Janeiro, Brazil., Dinis Ano Bom AP; Oswaldo Cruz Foundation, Biomanguinhos, Laboratory of Macromolecules, 21040-900, Rio de Janeiro, Rio de Janeiro, Brazil., Franco Sampaio AL; Oswaldo Cruz Foundation, Farmanguinhos, Institute of Pharmaceutical Technology, 21041-250, Rio de Janeiro, Rio de Janeiro, Brazil., Godinho da Silva J Jr; Oswaldo Cruz Foundation, Biomanguinhos, Laboratory of Macromolecules, 21040-900, Rio de Janeiro, Rio de Janeiro, Brazil., Ferrara MA; Oswaldo Cruz Foundation, Farmanguinhos, Drugs Technology Laboratory, 21041-250, Rio de Janeiro, Rio de Janeiro, Brazil., Pinto da Silva Bon E; Federal University of Rio de Janeiro, Institute of Chemistry, Department of Biochemistry, Enzyme Technology Laboratory, 21941-909, Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: elba1996@iq.ufrj.br., Perales J; Oswaldo Cruz Foundation, Oswaldo Cruz Institute, Toxinology Laboratory, 21040-900, Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: jperales@ioc.fiocruz.br.

Publikováno v: Protein expression and purification [Protein Expr Purif] 2016 Apr; Vol. 120, pp. 118-25. Date of Electronic Publication: 2015 Dec 20.