Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Gomathinayagam Ponniah"'
Autor:
Christine Nowak, Rekha Patel, Gomathinayagam Ponniah, Alyssa Neill, Hongcheng Liu, Cory King, Zhenyu Gu
Publikováno v:
Journal of Chromatography B. 1085:96-103
In-depth characterization of the commonly observed variants is critical to the successful development of recombinant monoclonal antibody therapeutics. Multiple peaks of a recombinant monoclonal antibody were observed when analyzed by hydrophobic inte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6939480b5429acf0b47a383b61a98f8
https://doi.org/10.1016/j.jchromb.2018.03.049
https://doi.org/10.1016/j.jchromb.2018.03.049
Publikováno v:
Analytical Biochemistry. 526:1-8
LC-MS peptide mapping is the most commonly used method to analyze protein modifications. The proteins are generally digested using trypsin at a slightly basic pH at 37 °C from several hours to overnight. Assay-induced artifacts can be generated duri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57989a7bd8b531c9052c9c7ec18b7f99
https://doi.org/10.1016/j.ab.2017.03.005
https://doi.org/10.1016/j.ab.2017.03.005
Publikováno v:
Analytical Biochemistry. 520:49-57
An efficient strategy to characterize recombinant monoclonal antibody charge variants was established using weak anion exchange chromatography, LC-MS and IdeS digestion to allow subunit level characterization. Significantly higher resolution was achi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a52ecc3f26226a628e12b25e39edcac
https://doi.org/10.1016/j.ab.2016.12.017
https://doi.org/10.1016/j.ab.2016.12.017
Publikováno v:
Biotechnology Progress. 32:1103-1112
Recombinant monoclonal antibodies are commonly expressed in mammalian cell culture and purified by several steps of filtration and chromatography. The resulting high purity bulk drug substance still contains product variants differing in properties s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8555c58e40faaa0c9538d501f68a8478
https://doi.org/10.1002/btpr.2327
https://doi.org/10.1002/btpr.2327
Autor:
Christine Nowak, Adriana Kita, Hongcheng Liu, Guilong Cheng, Gomathinayagam Ponniah, Alyssa Neill, Yekaterina Kori
Publikováno v:
Analytical Biochemistry. 496:4-8
Light exposure is one of several conditions used to study the degradation pathways of recombinant monoclonal antibodies. Tryptophan is of particular interest among the 20 amino acids because it is the most photosensitive. Tryptophan degradation forms
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::619825c934b1361ef1819712dec8d632
https://doi.org/10.1016/j.ab.2015.12.004
https://doi.org/10.1016/j.ab.2015.12.004
Autor:
Hongcheng Liu, Yekaterina Kori, Christine Nowak, Gomathinayagam Ponniah, Adriana Kita, Alyssa Neill, Saravanamoorthy Rajendran
Publikováno v:
Analytical Chemistry. 87:9084-9092
Charge variants, especially acidic charge variants, of recombinant monoclonal antibodies have been challenging to fully characterize despite the fact that several posttranslational modifications have already been identified. The acidic species of a r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebbc5fb0d07cdd846be66628a7b69011
https://doi.org/10.1021/acs.analchem.5b02385
https://doi.org/10.1021/acs.analchem.5b02385
Autor:
Gomathinayagam Ponniah, Alain Beck, Hongcheng Liu, Shara Dellatore, Joanne Sun, Bruce Mason, Ram Bhat, Amit Katiyar, Alyssa Neill, Jason K. Cheung, Christine Nowak
Forced degradation studies have become integral to the development of recombinant monoclonal antibody therapeutics by serving a variety of objectives from early stage manufacturability evaluation to supporting comparability assessments both pre- and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ca0bd3ee6367d7357bedf2367bdad0c
https://europepmc.org/articles/PMC5680805/
https://europepmc.org/articles/PMC5680805/
Autor:
Christine Nowak, Mei Shao, Alyssa Neill, Hongcheng Liu, Gomathinayagam Ponniah, Bruce A. Andrien
Publikováno v:
Biotechnology progress. 33(5)
Glycosylation of the conserved asparagine residue in the CH2 domain is the most common posttranslational modification of recombinant monoclonal antibodies. Ideally, a consistent oligosaccharide profile should be maintained from early clinical materia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a066cb1f6379031a032f8bfd12481ceb
https://pubmed.ncbi.nlm.nih.gov/28547754
https://pubmed.ncbi.nlm.nih.gov/28547754
Publikováno v:
Journal of Chromatography B. 958:90-95
Glycation is a common modification of proteins both in vitro and in vivo. To aid identification and comparative quantitation, a method of stable isotope labeling followed by LC–MS analysis was proposed. The samples were reduced using sodium borohyd
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c2df7e23d6a6ad95c7a7c3b7da00cf7
https://doi.org/10.1016/j.jchromb.2014.03.021
https://doi.org/10.1016/j.jchromb.2014.03.021
Accurate Determination of Protein Methionine Oxidation by Stable Isotope Labeling and LC-MS Analysis
Publikováno v:
Analytical Chemistry. 85:11705-11709
Methionine (Met) oxidation is a major modification of proteins, which converts Met to Met sulfoxide as the common product. It is challenging to determine the level of Met sulfoxide, because it can be generated during sample preparation and analysis a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4b9b4826fa9f081e76de36bb0377921
https://doi.org/10.1021/ac403072w
https://doi.org/10.1021/ac403072w