Zobrazeno 1 - 10
of 341
pro vyhledávání: '"Golden Gate cloning"'
Autor:
Takashi Watanabe, Hikaru Hata, Yoshiki Mochizuki, Fumie Yokoyama, Tomoko Hasegawa, Naveen Kumar, Tomohiro Kurosaki, Osamu Ohara, Hidehiro Fukuyama
Publikováno v:
eLife, Vol 13 (2024)
Antibodies are powerful tools for the therapy and diagnosis of various diseases. In addition to conventional hybridoma-based screening, recombinant antibody-based screening has become a common choice; however, its application is hampered by two facto
Externí odkaz:
https://doaj.org/article/7c8635ab87ed41a1accabbc80ff78a36
Autor:
Nurul Hidayah Samsulrizal, Anis Afuza Md Yusof, Amin-Asyraf Tamizi, Nurul Asyikin Mohd Zim, Siti Syafiqa Abdul Sattar, Mohd Syahmi Salleh, Nur Sabrina Ahmad Azmi, Zamri Zainal, Zarina Zainuddin
Publikováno v:
Journal of Tropical Life Science, Vol 13, Iss 2, Pp 257-276 (2023)
Rice (Oryza sativa) is a staple food consumed by the majority of the world’s population. Climate change, however, has created a significant threat to our food security as it posed severe effects on rice production. The emergence of genome editing t
Externí odkaz:
https://doaj.org/article/60df567fc6be4f909e2118480a820b9f
Akademický článek
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Akademický článek
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Autor:
Xiuyuan Wang, Chong Teng, Huitian Wei, Shuang Liu, Hongzhuan Xuan, Wentao Peng, Qianqian Li, Hongyan Hao, Qingya Lyu, Shanhua Lyu, Yinglun Fan
Publikováno v:
Frontiers in Plant Science, Vol 13 (2023)
With the advent of multiple omics and Genome-Wide Association Studies (GWAS) technology, genome-scale functional analysis of candidate genes is to be conducted in diverse plant species. Construction of plant binary expression vectors is the prerequis
Externí odkaz:
https://doaj.org/article/7338e523075542589e2cd404f87562b5
Akademický článek
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Autor:
David Fiebig, Jan P. Bogen, Stefania C. Carrara, Lukas Deweid, Stefan Zielonka, Julius Grzeschik, Björn Hock, Harald Kolmar
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)
Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approac
Externí odkaz:
https://doaj.org/article/07962c2c56b2491d9d889fe9fed966c1
Autor:
Jana Neuhold, Katharina Radakovics, Anita Lehner, Florian Weissmann, Maria Queralt Garcia, Mari Carmen Romero, Nicholas S. Berrow, Peggy Stolt-Bergner
Publikováno v:
BMC Biotechnology, Vol 20, Iss 1, Pp 1-15 (2020)
Abstract Background Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter
Externí odkaz:
https://doaj.org/article/2a6ec979be764c5d8a30b24ef358dfde
Autor:
Ya-Wen Li, Cai-Ling Yang, Qi Shen, Qian-Qian Peng, Qi Guo, Zhi-Kui Nie, Xiao-Man Sun, Tian-Qiong Shi, Xiao-Jun Ji, He Huang
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 9 (2022)
Non-homologous end-joining (NHEJ)-mediated random integration in Yarrowia lipolytica has been demonstrated to be an effective strategy for screening hyperproducer strains. However, there was no multigene assembly method applied for NHEJ integration,
Externí odkaz:
https://doaj.org/article/f519a83fde454b6e85577d1102c3669b
Publikováno v:
Plants, Vol 11, Iss 19, p 2620 (2022)
In vivo localization of proteins using fluorescence-based approaches by fusion of the protein of interest (POI) to a fluorescent protein is a cost- and time-effective tool to gain insights into its physiological function in a plant cell. Determining
Externí odkaz:
https://doaj.org/article/db01a0dd8c92445daf84fc9bab15d9a2