Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Gloria Y Lee"'
Publikováno v:
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injec
Externí odkaz:
https://doaj.org/article/d7fe4e1f335847248b103366c065b6ea
Publikováno v:
PLoS ONE, Vol 7, Iss 8, p e42267 (2012)
The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and stora
Externí odkaz:
https://doaj.org/article/8a939c1815ad4c6a85f48d1911b2f9bf
Publikováno v:
PLoS ONE, Vol 7, Iss 1, p e29924 (2012)
With the fast advancement in the genetics and bio-medical fields, the vast number of valuable transgenic and rare genetic mouse models need to be preserved. Preservation of mouse sperm by convective drying and subsequent storing at above freezing tem
Externí odkaz:
https://doaj.org/article/c58afff4d3c744fcbd972cc0a318a482
Publikováno v:
Cryobiology. 98
Vitrification of mammalian oocytes and embryos is typically a two-step procedure involving two solutions of increasing concentrations of cryoprotectants. In the present study, we report a simple vitrification protocol that uses low cryoprotectant con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc150facf42a9b1cc7037f91fcf13e5c
https://pubmed.ncbi.nlm.nih.gov/33137307
https://pubmed.ncbi.nlm.nih.gov/33137307
Autor:
Mehmet Toner, Diane L. Wright, Gloria Y. Lee, Jie Liu, Thomas L. Toth, Cigdem Tanrikut, John D. Biggers
Publikováno v:
Cryobiology. 73:162-167
Cryopreservation of human spermatozoa is a commonly used technique in assisted reproduction, however freezing low concentrations of sperm while maintaining adequate post-thaw motility remains a challenge. In an effort to optimize post-thaw motility y
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c2a070ac313c97f556e0e0cd692a151
https://doi.org/10.1016/j.cryobiol.2016.08.004
https://doi.org/10.1016/j.cryobiol.2016.08.004
Publikováno v:
Journal of Animal Science. 90:3739-3742
A piezo-driven pipette that includes a small amount of mercury to enhance efficiency is widely used for mouse intracytoplasmic sperm injection (ICSI). Unfortunately, the use of toxic mercury is not permitted in hospital facilities and alternatives to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::508dff9c78b1bd467b61a68ca069fbc4
https://doi.org/10.2527/jas.2012-5136
https://doi.org/10.2527/jas.2012-5136
Publikováno v:
Biology of Reproduction. 69:2059-2067
To better understand the differences in cytoskeletal organization between in vivo (IVO) and in vitro (IVM) matured oocytes, we analyzed remodeling of the centrosome-microtubule complex in IVO and IVM mouse oocytes. Fluorescence imaging revealed drama
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5516102dc2c422f5499c0d4f93da5e7a
https://doi.org/10.1095/biolreprod.103.020537
https://doi.org/10.1095/biolreprod.103.020537
Publikováno v:
PLoS ONE
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
PLoS ONE, Vol 9, Iss 6, p e99809 (2014)
The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76254687bb0b33fdf046149790213a96
https://pubmed.ncbi.nlm.nih.gov/24924588
https://pubmed.ncbi.nlm.nih.gov/24924588
Publikováno v:
Molecular and Cellular Endocrinology. 176:155-162
Ke 6 is a 17β-hydroxysteroid dehydrogenase (17βHSD) that is expressed in the kidneys and gonads. The expression of this gene is markedly reduced in three murine models of recessive polycystic kidney disease, a developmental disorder, where some nep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d6cce2987f2d5fee15f38afb28a3878
https://doi.org/10.1016/s0303-7207(00)00398-1
https://doi.org/10.1016/s0303-7207(00)00398-1
Publikováno v:
Journal of Biological Chemistry. 273:22664-22671
The abnormal regulation of the Ke 6 gene has been linked to the development of recessive polycystic kidney disease in the mouse. In this report, we have shown that Ke 6 is a 17beta-hydroxysteroid dehydrogenase and can regulate the concentration of bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be1502d69dcf3ed5bcaf6bad1e308d11
https://doi.org/10.1074/jbc.273.35.22664
https://doi.org/10.1074/jbc.273.35.22664