Various Strategies for the Immobilization of a Phospholipase C from Bacillus cereus for the Modulation of Its Biochemical Properties

Autor: Ines Abdelkader, Jose M. Guisán, Adel Sayari, Gloria Fernández-Lorente

Publikováno v: Molecules, Vol 29, Iss 7, p 1467 (2024)

In this study, the effect of various immobilization methods on the biochemical properties of phospholipase C (PLC) from Bacillus cereus obtained from the oily soil located in Sfax, Tunisia, was described. Different supports were checked: octyl sephar

Externí odkaz: https://doaj.org/article/717acad74afc49d7a7d63637290a2e7b

Stabilization of Lecitase Ultra® by Immobilization and Fixation of Bimolecular Aggregates. Release of Omega-3 Fatty Acids by Enzymatic Hydrolysis of Krill Oil

Autor: Daniel Andrés-Sanz, Cristina Fresan, Gloria Fernández-Lorente, Javier Rocha-Martín, Jose M. Guisán

Publikováno v: Catalysts, Vol 11, Iss 9, p 1067 (2021)

Lecitase Ultra® solutions are mainly composed of bimolecular aggregates of two open structures of the enzyme. The immobilization and fixation of these bimolecular aggregates onto support surfaces is here proposed as a novel protocol for the immobili

Externí odkaz: https://doaj.org/article/053fb85e9d344cb0b538a313f719f344

Immobilization of Eversa Lipases on Hydrophobic Supports for Production of Oleate Esters Solvent-Free

Autor: Gloria Fernández-Lorente, J. Vladimir Oliveira, Débora de Oliveira, Jorge Luiz Ninow, J. Manuel Guisan, Daniela Remonatto

Lipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2add81a399889125df6676e912d72500
https://doi.org/10.21203/rs.3.rs-710624/v1

Thermotolerant lipase from Penicillium sp. section Gracilenta CBMAI 1583: Effect of carbon sources on enzyme production, biochemical properties of crude and purified enzyme and substrate specificity

Autor: Eleonora Cano Carmona, César Rafael Fanchini Terrasan, Alex Fernando de Almeida, Cárol Cabral Terrone, Benevides C. C. Pessela, Daniela Flavia Machado Turati, Gloria Fernández-Lorente, Juliana Montesino de Freitas Nascimento, Jose M. Guisan

Publikováno v: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Digital.CSIC. Repositorio Institucional del CSIC
instname

In this study, Penicillium sp. section Gracilenta CBMAI 1583 was used to produce lipase under submerged conditions. The enzyme was purified and the biochemical properties of both the crude and purified enzymes were evaluated. Maximum lipase productio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b94a37f186ad028054f26c9ffd2adb3f
https://doi.org/10.1016/j.bcab.2018.10.002

Stabilization of Lecitase Ultra® by immobilization and fixation of bimolecular aggregates. Release of omega-3 fatty acids by enzymatic hydrolysis of krill oil

Autor: Javier Rocha-Martín, Jose M. Guisan, Daniel Andrés-Sanz, Gloria Fernández-Lorente, Cristina Fresan

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
Catalysts, Vol 11, Iss 1067, p 1067 (2021)
Catalysts
Volume 11
Issue 9

This article belongs to the Special Issue Application of Immobilized Enzyme as Catalysts in Chemical Synthesis.
Lecitase Ultra® solutions are mainly composed of bimolecular aggregates of two open structures of the enzyme. The immobilization and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3d5dacc390f59e58cfa98c8c37d9b9a
http://hdl.handle.net/10261/263406