An unmodified wobble uridine in tRNAs specific for Glutamine, Lysine, and Glutamic acid from Salmonella enterica Serovar Typhimurium results in nonviability-Due to increased missense errors?

Autor: Kristina Nilsson, Gunilla Jäger, Glenn R Björk

Publikováno v: PLoS ONE, Vol 12, Iss 4, p e0175092 (2017)

In the wobble position of tRNAs specific for Gln, Lys, and Glu a universally conserved 5-methylene-2-thiouridine derivative (xm5s2U34, x denotes any of several chemical substituents and 34 denotes the wobble position) is present, which is 5-(carboxy)

Externí odkaz: https://doaj.org/article/45384b37562f428589590957ea5d3a1b

The phenotype of many independently isolated +1 frameshift suppressor mutants supports a pivotal role of the P-site in reading frame maintenance.

Autor: Gunilla Jäger, Kristina Nilsson, Glenn R Björk

Publikováno v: PLoS ONE, Vol 8, Iss 4, p e60246 (2013)

The main features of translation are similar in all organisms on this planet and one important feature of it is the way the ribosome maintain the reading frame. We have earlier characterized several bacterial mutants defective in tRNA maturation and

Externí odkaz: https://doaj.org/article/caae4f26b59743d6aa0e06bef4652129

The output of the tRNA modification pathways controlled by theEscherichia coliMnmEG and MnmC enzymes depends on the growth conditions and the tRNA species

Autor: M.-Eugenia Armengod, M.-José Garzón, Glenn R. Björk, Ismaïl Moukadiri

Publikováno v: NUCLEIC ACIDS RESEARCH
r-CIPF. Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
instname
r-CIPF: Repositorio Institucional Producción Científica del Centro de Investigación Principe Felipe (CIPF)
Centro de Investigación Principe Felipe (CIPF)
Nucleic Acids Research

In Escherichia coli, the MnmEG complex modifies transfer RNAs (tRNAs) decoding NNA/NNG codons. MnmEG catalyzes two different modification reactions, which add an aminomethyl (nm) or carboxymethylaminomethyl (cmnm) group to position 5 of the anticodon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c91869d1228a1989e389951c142092a8
https://doi.org/10.1093/nar/gkt1228

Trm112p Is a 15-kDa Zinc Finger Protein Essential for the Activity of Two tRNA and One Protein Methyltransferases in Yeast

Autor: Léon Dirick, Bruno Lapeyre, Glenn R. Björk, Marie-Hélène Mazauric, Suresh K. Purushothaman

Publikováno v: Journal of Biological Chemistry
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, 285 (24), pp.18505-15. 〈10.1074/jbc.M110.113100〉
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, 285 (24), pp.18505-18515. ⟨10.1074/jbc.M110.113100⟩

International audience; The degenerate base at position 34 of the tRNA anticodon is the target of numerous modification enzymes. In Saccharomyces cerevisiae, five tRNAs exhibit a complex modification of uridine 34 (mcm(5)U(34) and mcm(5)s(2)U(34)), t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb9a8ae0da66b4af63c96ac15c38a69a
https://doi.org/10.1074/jbc.m110.113100

A Gripping Tale of Ribosomal Frameshifting: Extragenic Suppressors of Frameshift Mutations Spotlight P-Site Realignment

Autor: John F. Atkins, Glenn R. Björk

Publikováno v: Microbiology and Molecular Biology Reviews

SUMMARY Mutants of translation components which compensate for both −1 and +1 frameshift mutations showed the first evidence for framing malleability. Those compensatory mutants isolated in bacteria and yeast with altered tRNA or protein factors ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::198702abd9e2b89cc8b0c81fbd1725e4