Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Glenn G. Lilley"'
Publikováno v:
Protein Expression and Purification. 12:295-304
The cloning and expression of the Haemophilus influenzae gene, nanA, for the putative N-acetylneuraminate lyase enzyme, also known as N-acetylneuraminic acid aldolase or sialic acid aldolase, are reported. The gene was isolated from ATCC type strain
Autor:
Peter J. Hudson, Glenn G. Lilley, Lesley A. Pearce, Gregory Coia, Geoffrey W. Oddie, Alexander A. Kortt
Publikováno v:
IUBMB Life. 42:1179-1188
Monomeric single chain antibody (scFv) fragments lack both the avidity of the bivalent IgG, or (Fab')2 fragment, and the effector functions conferred by the Fc domain. For certain diagnostic or therapeutic applications it may be desirable to link the
Publikováno v:
Immunotechnology. 1:197-209
Background : The current format of a rapid whole-blood agglutination assay for HIV relies on a bifunctional molecule which comprises a 10 Fab fragment, with specificity for the human red blood cell surface marker (glycophorin A), chemically conjugate
Publikováno v:
Microbiology. 140:2093-2100
Dichelobacter nodosus, a Gram-negative obligate anaerobe and the causative agent of ovine footrot, secretes a number of extracellular proteases, one of which is highly basic in nature. The gene (bprV) encoding this basic protease, from virulent strai
Publikováno v:
Structure. 2:361-369
Background N -acetylneuraminate lyase catalyzes the cleavage of N -acetylneuraminic acid (sialic acid) to form pyruvate and N - acetyl- d -mannosamine. The enzyme plays an important role in the regulation of sialic acid metabolism in bacteria. The re
Publikováno v:
Biochemical Journal. 299:521-525
An extracellular serine proteinase with a PI approximately 9.5 (referred to as ‘basic proteinase’) was purified to homogeneity, from strains of Dichelobacter nodosus that cause virulent foot-rot, by gel filtration of concentrated culture supernat
Publikováno v:
European Journal of Biochemistry. 210:13-21
A DNA fragment encoding an extracellular basic protease (pI approximately 9.5) from Dichelobacter nodosus, a Gram-negative obligate anaerobe and the causative agent of ovine footrot, has been cloned and expressed in Escherichia coli and sequenced. E.
Publikováno v:
Protein Expression and Purification. 3:434-440
The Escherichia coli gene which encodes N-acetylneuraminic acid aldolase was isolated by the polymerase chain reaction, cloned into the inducible expression vector pTTQ18, and overexpressed in E. coli. The high yield of aldolase was achieved through
Publikováno v:
European Journal of Biochemistry. 195:329-334
The amino acid sequence of a methionine-rich 2S seed protein from sunflower (Helianthus annuus L.) and the sequence of a cDNA clone which codes for the entire primary translation product have been determined. The mature protein consists of a single p
Autor:
Sotirios Kolivas, Mauro Baldi, Elizabeth Johnson, Robert J. Blagrove, Glenn G. Lilley, Kenwyn R. Gayler, Alison J. Macfarlane
Publikováno v:
Plant Molecular Biology. 15:879-893
The biosynthesis of conglutin delta has been studied in developing cotyledons of Lupinus angustifolius L. Precursors of conglutin delta formed the major sink for [35S]-cysteine incorporated by developing lupin cotyledons, and these precursors were ra