Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Glen Teshima"'
Autor:
Christi Abbate, Karen L. Fearon, Tracy dela Cruz, Debbie Higgins, Gary Van Nest, David Passmore, Jason Marshall, Stephen Tuck, Gary Ott, Priscilla Yee, Glen Teshima
Publikováno v:
Cellular Immunology. 229:93-105
The immunostimulatory effects of bacterial DNA on mammalian cells have been localized to unmethylated CpG motifs, and synthetic CpG-containing oligodeoxynucleotides that mimic these effects are known as immunostimulatory sequences (ISS). We have foun
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::157d5f2381cd844b670c6aa8ebdfc3b6
https://doi.org/10.1016/j.cellimm.2004.04.009
https://doi.org/10.1016/j.cellimm.2004.04.009
Publikováno v:
Analytical Biochemistry. 244:221-227
Reports on nonenzymatic oxidation of human growth hormone (hGH) have been previously limited to methionyl residues (Met14and Met125). We report on the oxidation of a histidyl residue in hGH treated with intense light. The photooxidation process is pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b84006e43d10ff5cbbccfaaf87c2d9b
https://doi.org/10.1006/abio.1996.9899
https://doi.org/10.1006/abio.1996.9899
Autor:
Ming-Xiang Li, Jacob Kung, Carol Huang, Joel K. Freeberg, Vafa Lahidji, Chidi R. Obi, Lauren Thorner, Rahima Danishmand, Milan T. Tomic, Robert To, Glen Teshima
Monoclonal antibodies are subject to a variety of degradation mechanisms, therefore orthogonal techniques are required to demonstrate product quality. In this study, the three individual antibodies comprising a multi-antibody drug product, XOMA 3AB w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec5f810733a9d006c8944e89d56c451c
https://europepmc.org/articles/PMC3065544/
https://europepmc.org/articles/PMC3065544/
Autor:
Eleanor Canova-Davis, Glen Teshima
Publikováno v:
Journal of Chromatography A. 625:207-215
Human growth hormone (hGH), a polypeptide of 191 amino acids, contains three methionine residues, two of which are susceptible to oxidation by both chemical and photochemical means (Met14 and Met125). To date, no method has existed for resolving the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7e39bdcd5685cffaedc261df4673e61
https://doi.org/10.1016/0021-9673(92)85204-7
https://doi.org/10.1016/0021-9673(92)85204-7
Publikováno v:
Journal of Biological Chemistry. 266:13544-13547
Deamidation of asparagine and glutamine residues, isomerization of aspartic acid side chains, and racemization of the L- to the D-form of the amino acids are common spontaneous chemical reactions known to occur in proteins. Previous studies have impl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caef5e40e3151c9f5e4913861e87990c
https://doi.org/10.1016/s0021-9258(18)92732-8
https://doi.org/10.1016/s0021-9258(18)92732-8
Publikováno v:
Biochemistry. 30:3916-3922
High-performance cation-exchange chromatography of recombinant soluble CD4 (rCD4) allowed the resolution of four charge variants. This charge heterogeneity could be eliminated by neuraminidase treatment of rCD4 and therefore can be attributed to diff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::014999a0eab17e336ba5494586335f97
https://doi.org/10.1021/bi00230a016
https://doi.org/10.1021/bi00230a016
Autor:
Glen Teshima, Venkat R. Mukku, Melinda Marian, Todd C. Somers, Germaine Fuh, James A. Wells, Michael J. Cronin, Marjorie E. Winkler, Suh Chang, Eleanor Canova-Davis, Herman Chu, Ross G. Clark, Deborah L. Mortensen, Kenneth Charles Olson
Publikováno v:
The Journal of biological chemistry. 271(36)
Derivatives of human growth hormone (hGH) of increasing size were produced by reaction with the N-hydroxysuccinimide ester of polyethylene glycol-5000 (PEG5000), a 5-kDa reagent that selectively conjugates to primary amines. By adjusting the reaction
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60f7189245dbcfa6dc1cee6037c89a8
https://pubmed.ncbi.nlm.nih.gov/8703002
https://pubmed.ncbi.nlm.nih.gov/8703002
Autor:
Shiaw-Lin Wu, Glen Teshima
SDS-PAGE is one of the most widely used techniques for the analysis of proteins, owing to the importance of determining molecular weight. The capillary electrophoresis sieving technique described here can also provide molecular weight data and with g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e4a7ee1371ca248b6fbb2fb94bf00694
https://doi.org/10.1016/s0076-6879(96)71014-5
https://doi.org/10.1016/s0076-6879(96)71014-5
Autor:
William S. Hancock, Barry L. Karger, Stanislav. Wicar, Shiaw Lin Wu, Glen Teshima, Peter. Oroszlan
Publikováno v:
Analytical chemistry. 64(14)
This paper examines the retention behavior of recombinant DNA-derived human growth hormone (rhGH) in reversed-phase chromatography and its separation from the closely related N-methionyl variant (Met-hGH). It is first shown that retention for rhGH de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05191cd24f748869a43387293c1b29fe
https://pubmed.ncbi.nlm.nih.gov/1503227
https://pubmed.ncbi.nlm.nih.gov/1503227
Publikováno v:
Journal of chromatography. 516(1)
The separation of charge variants of recombinant DNA-derived (rDNA) proteins by high-performance capillary electrophoresis (HPCE) has been explored with the following examples: human growth hormone (rhGH), a soluble form of a T4 receptor protein (rCD
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d184f04ee5678ec51c73a9a5069de5c
https://pubmed.ncbi.nlm.nih.gov/2126790
https://pubmed.ncbi.nlm.nih.gov/2126790