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Current outcomes when optimizing 'standard' sample preparation for single-particle cryo-EM

Autor: Carragher, B, Cheng, Y, Frost, A, Glaeser, RM, Lander, GC, Nogales, E, Wang, H-W

Publikováno v: Journal of microscopy, vol 276, iss 1

Although high-resolution single-particle cryo-electron microscopy (cryo-EM) is now producing a rapid stream of breakthroughs in structural biology, it nevertheless remains the case that the preparation of suitable frozen-hydrated samples on electron

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::89090ef81ca2a78316738535c5403cea
https://escholarship.org/uc/item/73w2x3hf

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How Cryo-EM Became so Hot

Autor: Cheng, Y, Glaeser, RM, Nogales, E

Publikováno v: Cheng, Y; Glaeser, RM; & Nogales, E. (2017). How Cryo-EM Became so Hot. Cell, 171(6), 1229-1231. doi: 10.1016/j.cell.2017.11.016. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/8mb8h8nv

© 2017 Elsevier Inc. The Royal Swedish Academy of Sciences awarded the 2017 Nobel Prize for Chemistry to Jacques Dubochet, Joachim Frank, and Richard Henderson for “developing cryoelectron microscopy for the high-resolution structure determination

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http://www.escholarship.org/uc/item/8mb8h8nv

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Near-concentric Fabry-P rot cavity for continuous-wave laser control of electron waves

Autor: Schwartz, O, Axelrod, JJ, Tuthill, DR, Haslinger, P, Ophus, C, Glaeser, RM, Müller, H

Publikováno v: Schwartz, O; Axelrod, JJ; Tuthill, DR; Haslinger, P; Ophus, C; Glaeser, RM; et al.(2017). Near-concentric Fabry-P rot cavity for continuous-wave laser control of electron waves. Optics Express, 25(13), 14453-14462. doi: 10.1364/OE.25.014453. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/6fx8r37g

© 2017 Optical Society of America. Abstract: Manipulating free-space electron wave functions with laser fields can bring about new electron-optical elements for transmission electron microscopy (TEM). In particular, a Zernike phase plate would enabl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::7140c3d903f774ccda207c851389a92f
http://www.escholarship.org/uc/item/6fx8r37g

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Opinion: hazards faced by macromolecules when confined to thin aqueous films

Autor: Glaeser, RM, Han, B-G

Publikováno v: Glaeser, RM; & Han, B-G. (2017). Opinion: hazards faced by macromolecules when confined to thin aqueous films.. Biophysics reports, 3(1), 1-7. doi: 10.1007/s41048-016-0026-3. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/43f7p67n

Samples prepared for single-particle electron cryo-microscopy (cryo-EM) necessarily have a very high surface-to-volume ratio during the short period of time between thinning and vitrification. During this time, there is an obvious risk that macromole

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http://www.escholarship.org/uc/item/43f7p67n

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Specimen Behavior in the Electron Beam

Autor: Glaeser, RM

Publikováno v: Glaeser, RM. (2016). Specimen Behavior in the Electron Beam. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/6tf7f3vh

© 2016 Elsevier Inc. It has long been known that cryo-EM specimens are severely damaged by a level of electron exposure that is much lower than what is needed to obtain high-resolution images from single macromolecules. Perhaps less well appreciated

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::15890caef728140a5fe42415a7419080
https://escholarship.org/uc/item/6tf7f3vh

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Ranking TEM cameras by their response to electron shot noise

Autor: Grob, P, Bean, D, Typke, D, Li, X, Nogales, E, Glaeser, RM

Publikováno v: Grob, P; Bean, D; Typke, D; Li, X; Nogales, E; & Glaeser, RM. (2013). Ranking TEM cameras by their response to electron shot noise. Ultramicroscopy, 133, 1-7. doi: 10.1016/j.ultramic.2013.01.003. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/38t2t549

We demonstrate two ways in which the Fourier transforms of images that consist solely of randomly distributed electrons (shot noise) can be used to compare the relative performance of different electronic cameras. The principle is to determine how cl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::0679eb74cdaa91d4cdfa463494b87b4b
https://escholarship.org/uc/item/38t2t549

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Crystallographic extraction and averaging of data from small image areas

Autor: Perkins, GA, Downing, KH, Glaeser, RM

Publikováno v: Ultramicroscopy, 60(2), 283-294. ELSEVIER SCIENCE BV

The accuracy of structure factor phases determined from electron microscope images is determined mainly by the level of statistical significance, which is limited by the low level of allowed electron exposure and by the number of identical unit cells

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https://research.rug.nl/en/publications/7f2b0175-4f00-4dbc-ae63-7c5caa5c6271

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