Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Gisele Fernanda Assine Picchi"'
Autor:
Eloise Pavão Guerra-Slompo, Gisele Fernanda Assine Picchi-Constante, Martin Marek, Christophe Romier, Wolfgang Sippl, Nilson Ivo Tonin Zanchin
Publikováno v:
STAR Protocols, Vol 4, Iss 1, Pp 102058- (2023)
Summary: Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while ph
Externí odkaz:
https://doaj.org/article/a6ce12f930fd45ce85f6f3a2270026cc
Autor:
Gisele Fernanda Assine Picchi-Constante, Eloise Pavão Guerra-Slompo, Ana Carolina Tahira, Monica Visnieski Alcantara, Murilo Sena Amaral, Arthur Schveitzer Ferreira, Michel Batista, Cassiano Martin Batista, Samuel Goldenberg, Sergio Verjovski-Almeida, Nilson Ivo Tonin Zanchin
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
Abstract Trypanosoma cruzi—the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene expression. However, trypanosomatids undergo drastic changes in nuclear architectu
Externí odkaz:
https://doaj.org/article/096465a371e64559b0aaba9b89558b88
Autor:
Gisele Fernanda Assine Picchi-Constante, Priscila Mazzocchi Hiraiwa, Martin Marek, Vanessa Zulkievicz Rogerio, Eloise Pavão Guerra-Slompo, Christophe Romier, Nilson Ivo Tonin Zanchin
Publikováno v:
STAR Protocols, Vol 3, Iss 2, Pp 101324- (2022)
Summary: This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene contain
Externí odkaz:
https://doaj.org/article/5233e0e1faa14a838c7f1926e6da97ab
Publikováno v:
Memorias do Instituto Oswaldo Cruz, Vol 109, Iss 4, Pp 511-513 (2014)
Gene knockout is a widely used approach to evaluate loss-of-function phenotypes and it can be facilitated by the incorporation of a DNA cassette having a drug-selectable marker. Confirmation of the correct knockout cassette insertion is an important
Externí odkaz:
https://doaj.org/article/2632c8a8eed2473b8538a250af411528
Autor:
Cassiano Martin Batista, Gisele Fernanda Assine Picchi-Constante, Ana Carolina Tahira, Nilson Ivo Tonin Zanchin, Murilo S. Amaral, Michel Batista, Arthur Schveitzer Ferreira, Samuel Goldenberg, Sergio Verjovski-Almeida, Eloise P. Guerra-Slompo, Monica Visnieski Alcantara
Publikováno v:
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
Scientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
Trypanosoma cruzi—the causative agent of Chagas disease—like other kinetoplastids, relies mostly on post-transcriptional mechanisms for regulation of gene expression. However, trypanosomatids undergo drastic changes in nuclear architecture and ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee5582f53fb1ecf71d615d3598f3912f
https://pubmed.ncbi.nlm.nih.gov/34737385
https://pubmed.ncbi.nlm.nih.gov/34737385
Autor:
Alessandra A. Guarneri, Rafael Luis Kessler, Stenio Perdigão Fragoso, Gisele Fernanda Assine Picchi, Rosana Elisa Gonçalves Gonçalves, Newmar Pinto Marliére, Monica Visnieski Alcantara
Publikováno v:
Molecular and Biochemical Parasitology. 221:1-9
In the protozoan parasite Trypanosoma cruzi - the causative agent of Chagas disease - gene expression control is mainly post-transcriptional, where RNA-binding proteins (RBPs) play a central role, by controlling mRNA stability, distribution and trans
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76a9dd75b70e8d01109ee7983d5cba52
https://doi.org/10.1016/j.molbiopara.2018.01.006
https://doi.org/10.1016/j.molbiopara.2018.01.006
Autor:
Samuel Goldenberg, Vanessa Zulkievicz, Marco Aurélio Krieger, Nilson Ivo Tonin Zanchin, Lyris M. F. de Godoy, Gisele Fernanda Assine Picchi
Publikováno v:
Journal of Proteome Research. 16:1167-1179
Chagas disease, caused by Trypanosoma cruzi, still affects millions of people around the world. No vaccines nor treatment for chronic Chagas disease are available, and chemotherapy for the acute phase is hindered by limited efficacy and severe side e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2feb9c84bc5bb00adc87cd9e4bbfcafb
https://doi.org/10.1021/acs.jproteome.6b00655
https://doi.org/10.1021/acs.jproteome.6b00655
Autor:
Samuel Goldenberg, Gisele Fernanda Assine Picchi, Priscila M. Hiraiwa, Lysangela R. Alves, Beatriz S. Borges, Bruno Accioly Alves Romagnoli
Publikováno v:
Acta tropica. 178
Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects millions of individuals around the world. Although it has been known for more than a century, the study of T. cruzi has been a challenge, particularly due to the scarcity of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cd2e2a8f4050d5445a956a0148ddf2f
https://pubmed.ncbi.nlm.nih.gov/29174293
https://pubmed.ncbi.nlm.nih.gov/29174293
Autor:
Karina Goulart dos Anjos, Emanuel Maltempi de Souza, Gisele Fernanda Assine Picchi, Helisson Faoro, Marcelo Rogalski, Miguel Pedro Guerra, Fábio O. Pedrosa, Rubens Onofre Nodari, Leila do Nascimento Vieira, Hugo Pacheco de Freitas Fraga
Publikováno v:
Tree Genetics & Genomes. 12
Retrophyllum piresii (Podocarpaceae) is an endemic conifer species from the Brazilian Amazonian Region, and very few data related to ecological and genetic characteristics of this species are available. Plastome sequencing is an efficient tool to und
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::31368c6028077eb37516cc2e278ef04d
https://doi.org/10.1007/s11295-016-0968-0
https://doi.org/10.1007/s11295-016-0968-0
Autor:
Samuel Goldenberg, Wanderley de Souza, Stenio Perdigão Fragoso, Danielle Pereira Cavalcanti, Gisele Fernanda Assine Picchi, Maria Cristina M. Motta, Édio Elígio Lourenço, Marco Aurélio Krieger
Publikováno v:
FEMS Microbiology Letters. 257:163-170
DNA topoisomerases are involved in DNA metabolism. These enzymes are inhibited by antimicrobial and antitumoral agents and might be important targets in the chemotherapy of diseases caused by parasites. We have cloned and characterized the gene encod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4188e14a4a7985748e09dec0e2d74406
https://doi.org/10.1111/j.1574-6968.2006.00164.x
https://doi.org/10.1111/j.1574-6968.2006.00164.x