Identification of a host cell protein impurity in therapeutic protein, P1

Autor: Girija Krishnamurthy, Sheetal Mehta, Lei Zhang, Harbhajan Dhillon, Hangtian Song, Heather Boux, Deepti Ahluwalia, Anulfo Valdez, Thomas R. Slaney

Publikováno v: Journal of Pharmaceutical and Biomedical Analysis. 141:32-38

Residual host cell proteins (HCPs) are process-related impurities present in biotherapeutics that can pose safety health risks to patients. An adequate control of HCP levels in the final product, and demonstration of HCP clearance throughout a produc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::659109dda6bd3b75dda6e869b63168b4
https://doi.org/10.1016/j.jpba.2017.03.065

Potent α-Synuclein Aggregation Inhibitors, Identified by High-Throughput Screening, Mainly Target the Monomeric State

Autor: Christian Moestrup Jessen, Keith Pitts, Hossein Mohammad-Beigi, Martin Kurnik, Nikolai Lorenzen, Roland G.W. Staal, Cagla Sahin, Daniel E. Otzen, Scot Richard Mente, Frans A. A. Mulder, Jan Skov Pedersen, Camilla Bertel Andersen, Lise Giehm, Warren D. Hirst, Girija Krishnamurthy, Gunna Christiansen, Peter H. Reinhart, Steen V. Petersen

Publikováno v: Kurnik, M, Sahin, C, Andersen, C B, Lorenzen, N, Giehm, L, Mohammad-Beigi, H, Jessen, C M, Pedersen, J S, Christiansen, G, Petersen, S V, Staal, R, Krishnamurthy, G, Pitts, K, Reinhart, P H, Mulder, F A A, Mente, S, Hirst, W D & Otzen, D E 2018, ' Potent α-Synuclein Aggregation Inhibitors, Identified by High-Throughput Screening, Mainly Target the Monomeric State ', Cell Chemical Biology, vol. 25, no. 11, pp. 1389-1402 . https://doi.org/10.1016/j.chembiol.2018.08.005

α-Synuclein (αSN) aggregation is central to the etiology of Parkinson's disease (PD). Large-scale screening of compounds to identify aggregation inhibitors is challenged by stochastic αSN aggregation and difficulties in detecting early-stage oligo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d6e9912e43fa58913d068c4c4085319
https://pure.au.dk/portal/da/publications/potent-synuclein-aggregation-inhibitors-identified-by-highthroughput-screening-mainly-target-the-monomeric-state(5db8c797-9129-4ffc-9d50-99cacae6acc6).html

Drug-to-antibody determination for an antibody-drug-conjugate utilizing cathepsin B digestion coupled with reversed-phase high-pressure liquid chromatography analysis

Autor: Guoyong Sun, Wenkui Lan, Mark S. Bolgar, Hangtian Song, Difei Qiu, Amit Katiyar, Michael Adamo, Girija Krishnamurthy, Joseph J. Valente

Publikováno v: Journal of chromatography. A. 1481

Antibody drug conjugates or ADCs are currently being evaluated for their effectiveness as targeted chemotherapeutic agents across the pharmaceutical industry. Due to the complexity arising from the choice of antibody, drug and linker; analytical meth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f69da5d56122d8785a3e425742ee3fa
https://pubmed.ncbi.nlm.nih.gov/28017567

Pyranonaphthoquinone Lactones: A New Class of AKT Selective Kinase Inhibitors Alkylate a Regulatory Loop Cysteine

Autor: Cilien Hanna, Clark Eid, Erick Honores, Weiguo Zhang, Leif M. Laakso, Ayako Yamashita, Bernard Dean Johnson, Leonard A. McDonald, Zhong Li, Weidong Ding, Dennis Powell, Jeremy I. Levin, Girija Krishnamurthy, Keiko Tabei, Jaechul Shim, Edward J. Salaski, Shabana Insaf, Lourdes Toral-Barza, Ker Yu, Tarek S. Mansour

Publikováno v: Journal of Medicinal Chemistry. 52:2181-2184

The naturally occurring pyranonaphthoquinone (PNQ) antibiotic lactoquinomycin and related aglycones were found to be selective inhibitors of the serine-threonine kinase AKT. A set of synthetic PNQs were prepared and a minimum active feature set and p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5485ad3ec1278f1fbce482a2a467aff3
https://doi.org/10.1021/jm900075g

High-Throughput Screening for the Discovery of Inhibitors of Fatty Acid Amide Hydrolase Using a Microsome-Based Fluorescent Assay

Autor: Kathryn E. Rogers, Jun Xu, Manjunath K. Ramarao, Girija Krishnamurthy, Fernando Ramirez, Adam M. Gilbert, Gary Kalgaonkar, Wade Edris, Yuren Wang, Nina Kadakia, Philip G. Jones

Publikováno v: SLAS Discovery. 11:519-527

Fatty acid amide hydrolase (FAAH) is a membrane-associated enzyme that catalyzes the hydrolysis of several endogenous bioactive lipids, including anandamide (AEA), N-palmitoylethanolamine (PEA), oleamide, and N-oleoylethanolamine (OEA). These fatty a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::430e6b33efa90aa3319854517aaa27c4
https://doi.org/10.1177/1087057106288188