Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Giovanni Gragnaniello"'
Publikováno v:
PLoS ONE, Vol 7, Iss 6, p e39231 (2012)
Ionomycin is a Ca(2+)-selective ionophore that is widely used to increase intracellular Ca(2+) levels in cell biology laboratories. It is also occasionally used to activate eggs in the clinics practicing in vitro fertilization. However, neither the p
Externí odkaz:
https://doaj.org/article/44288e1cb1fa41459a376f8931b30c2b
Autor:
Jong T Chun, Agostina Puppo, Filip Vasilev, Giovanni Gragnaniello, Ezio Garante, Luigia Santella
Publikováno v:
PLoS ONE, Vol 5, Iss 11, p e14100 (2010)
BACKGROUND: Fertilization of echinoderm eggs is accompanied by dynamic changes of the actin cytoskeleton and by a drastic increase of cytosolic Ca(2+). Since the plasma membrane-enriched phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) serve
Externí odkaz:
https://doaj.org/article/40f8998b1de441e591ce1cff2b7de1b7
Publikováno v:
PLoS ONE, Vol 3, Iss 10, p e3588 (2008)
BackgroundWhen preparing for fertilization, oocytes undergo meiotic maturation during which structural changes occur in the endoplasmic reticulum (ER) that lead to a more efficient calcium response. During meiotic maturation and subsequent fertilizat
Externí odkaz:
https://doaj.org/article/7df95d5b64434679bf0cb843bbdef966
Publikováno v:
Molecular Reproduction and Development. 59:97-105
The purpose of the present study was to determine whether Octopus vulgaris spermatozoa are activated by progesterone stimulation. Spermatozoa were collected from the spermatophores in the Needham's sac of the male lMSr and from the spermathecae of ov
Publikováno v:
Biochemical and Biophysical Research Communications. 252:1-4
The dynamics of the cytoplasmic and nuclear Ca2+ pools in starfish oocytes arrested at the prophase of the first meiotic division or after induction of meiosis by 1-methyladenine (1-MA) have been studied by confocal microscopy. A 70 kDa fluorescent C
Publikováno v:
PLoS ONE
PLoS ONE, Vol 7, Iss 6, p e39231 (2012)
PLoS ONE, Vol 7, Iss 6, p e39231 (2012)
Ionomycin is a Ca(2+)-selective ionophore that is widely used to increase intracellular Ca(2+) levels in cell biology laboratories. It is also occasionally used to activate eggs in the clinics practicing in vitro fertilization. However, neither the p
Autor:
Luigia Santella, Giovanni Gragnaniello, Jong Tai Chun, Agostina Puppo, Filip Vasilev, Ezio Garante
Publikováno v:
PLoS ONE
PLoS ONE, Vol 5, Iss 11, p e14100 (2010)
PLoS ONE, Vol 5, Iss 11, p e14100 (2010)
Background Fertilization of echinoderm eggs is accompanied by dynamic changes of the actin cytoskeleton and by a drastic increase of cytosolic Ca(2+). Since the plasma membrane-enriched phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) serves
Autor:
Gianfranco Fenzi, Lucio Pastore, Maria Rosaria Rusciano, Sara Monaco, Mario Vitale, Guido Rossi, Eleonora Leggiero, Giovanni Gragnaniello, Maddalena Illario, Gaetano Di Spigna
Publikováno v:
Cell cycle (Georgetown, Tex.). 8(13)
Insulin effects are mediated by multiple integrated signals generated by the insulin receptor. Fibroblasts, as most of mammalian cells, are a target of insulin action and are important actors in the vascular pathogenesis of hyperinsulinemia. A role f
Publikováno v:
FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 15(12)
We have explored the role of the recently discovered second messenger nicotinic acid adenine nucleotide phosphate (NAADP+) in Ca2+ swings that accompany the fertilization process in starfish oocytes. The injection of NAADP+ deep into the cytoplasm of
Publikováno v:
Experimental cell research. 248(2)
Cortical granules (secretory vesicles located under the cortex of mature oocytes) release their contents to the medium at fertilization. Their exocytosis modifies the extracellular environment, blocking the penetration of additional sperm. The granul