Zobrazeno 1 - 10
of 62
pro vyhledávání: '"Giorgio Raspi"'
Autor:
Alessandro D'Ulivo, Roberto Zamboni, Chandra Sortino, Massimo Onor, Cristina Lomonte, Giorgio Raspi, Emilia Bramanti
Publikováno v:
Talanta. 63:383-389
Hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC–CVGAFS) has been optimized for the analysis of thiolic proteins in denaturing conditions. Proteins are pre-column simultaneously denatu
Autor:
Wes W. C. Quigley, Chandra Sortino, Emilia Bramanti, Massimo Onor, Giorgio Raspi, Francesca Beni, Robert E. Synovec
Publikováno v:
info:cnr-pdr/source/autori:Emilia Bramanti, Wes W. C. Quigley, Chandra Sortino, Francesca Beni, Massimo Onor, Giorgio Raspi and Robert E. Synovec/titolo:Multidimensional Analysis of Denatured Milk Proteins by Hydrophobic Interaction Chromatography coupled to a Dynamic Surface Tension Detector/doi:/rivista:/anno:2004/pagina_da:79/pagina_a:91/intervallo_pagine:79–91/volume:1023
Multidimensional analysis of denatured milk proteins is reported using high-performance liquid chromatography (HPLC) combined with dynamic surface tension detection (DSTD). A hydrophobic interaction chromatography (HIC) column (a TSK-Gel Phenyl-5PW c
Publikováno v:
Journal of Chromatography A. 958:157-166
Separation and determination of denatured alpha-, beta- and kappa-caseins by hydrophobic interaction chromatography (HIC) was improved by using a TSK-Gel Ether-5PW column (Tosoh Biosep). The method, already proposed and performed by a TSK-Gel Phenyl-
Publikováno v:
Chromatographia. 55:693-699
A new method for the direct detection of trypsin, chymotrypsin A and kallikrein from porcine pancreas by capillary zone electrophoresis is proposed. Optimum conditions for quantitative determination were investigated which also allow the unequivocal
Publikováno v:
Polyhedron. 21:1475-1479
Bubble extraction combined with affinity chromatography can be considered as fully reliable techniques to concentrate and separate proteins and enzymes, and could be applied to the treatment of large samples to purify analytes from diluted solutions,
Publikováno v:
Polyhedron. 21:1405-1410
A method based on the use of capillary zone electrophoresis (CZE) is proposed for the determination of the heterologous protein bovine pancreatic trypsin inhibitor (BPTI) expressed in yeast. Saccharomyces cerevisiae cells were transformed with plasmi
Autor:
Alessandro D'Ulivo, Emilia Bramanti, Leonardo Lampugnani, Maria Spinetti, Roberto Zamboni, Serena Lucchesini, Giorgio Raspi
Publikováno v:
J. Anal. At. Spectrom.. 16:166-171
A new analytical method is proposed for the determination and characterization of thiolic proteins, based on hydrophobic interaction chromatography (HIC) coupled on-line with cold vapour atomic fluorescence spectrometry (CVAFS). Thiolic groups are de
Publikováno v:
Analyst (Lond., 1877, Online) 126 (2001): 997–1000. doi:10.1039/b102360h
info:cnr-pdr/source/autori:Bramanti, E (Bramanti, E); Sortino, C (Sortino, C); Raspi, G (Raspi, G); Synovec, RE (Synovec, RE)/titolo:Separation and determination of denatured caseins by hydrophobic interaction chromatography-Part II. Method validation and applications/doi:10.1039%2Fb102360h/rivista:Analyst (Lond., 1877, Online)/anno:2001/pagina_da:997/pagina_a:1000/intervallo_pagine:997–1000/volume:126
info:cnr-pdr/source/autori:Bramanti, E (Bramanti, E); Sortino, C (Sortino, C); Raspi, G (Raspi, G); Synovec, RE (Synovec, RE)/titolo:Separation and determination of denatured caseins by hydrophobic interaction chromatography-Part II. Method validation and applications/doi:10.1039%2Fb102360h/rivista:Analyst (Lond., 1877, Online)/anno:2001/pagina_da:997/pagina_a:1000/intervallo_pagine:997–1000/volume:126
A method recently described for the separation of denatured alpha-, beta- and kappa -caseins by hydrophobic interaction chromatography was validated by the analysis of reference skim milk powder (BCR-063R) certificated for total nitrogen content. The
Publikováno v:
The Analyst. 124:511-515
Aldolase, glyceraldehyde 3-phosphate dehydrogenase and ovalbumin were determined by hydrophobic interaction chromatography, which allows the evaluation of the number of –SH groups per molecule of protein in both the native and denatured form. These
Publikováno v:
Chromatographia. 49:47-53
The use of hydrophobic-interaction chromatography (HIC) is proposed for the simultaneous determination of more than one thiol-protein after formation of the corresponding mercury mercaptides withp-hydroxymercuribenzoate (PHMB). The new chromatographi