Role of methoxypolyethylene glycol on the hydration, activity, conformation and dynamic properties of a lipase in a dry film

Autor: Gabriel Barletta, Giorgio Mazzola, Francesco Secundo

Publikováno v: Biotechnology and bioengineering
info:cnr-pdr/source/autori:Francesco Secundo (1); Gabriel Barletta (2); Giorgio Mazzola (3)/titolo:Role of methoxypolyethylene glycol on the hydration, activity, conformation and dynamic properties of a lipase in a dry film/doi:10.1002%2Fbit.21888/rivista:Biotechnology and bioengineering (Print)/anno:2008/pagina_da:255/pagina_a:262/intervallo_pagine:255–262/volume:101

A combined approach based on the use of ATR-FT/IR and steady-state fluorescence spectroscopy allowed to shed light on the effects of the additive methoxypolyethylene glycol (MePEG) on the hydration, conformation and dynamic properties of lipase from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e80982130a10832dde2b91bc2ccfe38e
https://doi.org/10.1002/bit.21888

Chemical modification of 3.alpha.,20.beta.-hydroxysteroid dehydrogenase with diethyl pyrocarbonate. Evidence for an essential, highly reactive, lysyl residue

Autor: Piero Pasta, Giorgio Mazzola, Giacomo Carrea

Publikováno v: Biochemistry. 26:1247-1251

Diethyl pyrocarbonate inactivated the tetrameric 3 alpha,20 beta-hydroxysteroid dehydrogenase with second-order rate constants of 1.63 M-1 s-1 at pH 6 and 25 degrees C or 190 M-1 s-1 at pH 9.4 and 25 degrees C. The activity was slowly and partially r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb52f406e5ae6569153b7c38d3b231fa
https://doi.org/10.1021/bi00379a007

The binding site for α-bungarotoxin resides in the sequence 188–201 of the α-subunit of acetylcholine receptor: Structure, conformation and binding characteristics of peptide [Lys] 188–201

Autor: Cecilia Gotti, Renato Longhi, Giorgio Mazzola, Diego Fornasari, Francesco Clementi

Publikováno v: Neuroscience Letters. 82:113-120

In order to study where the binding site of cholinergic agents is in the sequence of the alpha-subunit of nicotinic acetylcholine receptor (AChR), we have synthetized 3 peptides with an amino acid sequence corresponding to the following sequences of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5529ca9e1050f2118516ca6215fe764f
https://doi.org/10.1016/0304-3940(87)90180-7

Denaturation by urea and renaturation of 20β-hydroxysteroid dehydrogenase studied by high-performance size exclusion chromatography

Autor: Giorgio Mazzola, Piero Pasta, Giacomo Carrea, Renato Longhi, Giuseppe Vecchio

Publikováno v: Analytical Biochemistry. 180:181-185

The denaturation by urea and renaturation of 20β-hydroxysteroid dehydrogenase, a tetrameric enzyme consisting of four identical subunits, were followed by high-performance size exclusion chromatography to detect intermediates in the processes. Durin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f669b86abed54fea9315dcd3e17550fd
https://doi.org/10.1016/0003-2697(89)90109-7

Separation of choline- and ethanolamine-labeled metabolites by ion-exchange high-pressure liquid chromatography

Autor: Giorgio Mazzola, Claudia Kent

Publikováno v: Analytical biochemistry. 141(1)

Two methods utilizing ion-exchange high-performance liquid chromatography have been developed for the separation of choline- and ethanolamine-containing metabolic precursors for phosphatidylcholine and phosphatidylethanolamine. Both methods employ an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63a309aaa39401dbb79c0137741e8d3a
https://pubmed.ncbi.nlm.nih.gov/6541884