Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Gillian M. Stephens"'
Publikováno v:
Enzyme and Microbial Technology. 25:810-819
Production of toluene cis-glycol (TCG) was investigated using recombinant Escherichia coli strains that express toluene dioxygenase under the tac promoter. E. coli TG2 was selected as the host for the recombinant plasmid, pTAC365, because the TCG yie
Autor:
Gillian M. Stephens, E.Timothy Davies
Publikováno v:
Enzyme and Microbial Technology. 23:129-132
Acetobacterium woodii was grown on fructose, methanol, 3,4,5-trimethoxycinnamate, or 3-(3,4,5-trimethoxyphenyl)-propanoate in the presence or absence of caffeate. The cells were harvested and tested for their ability to hydrogenate CC bonds using
Publikováno v:
Microbiology. 143:2321-2329
Summary: An o-xylene-utilizing Rhodococcus, strain B3, was isolated from enrichments with o-xylene. The pathway for o-xylene degradation was investigated by simultaneous adaptation experiments, studies of product formation by a mutant and fortuitous
Publikováno v:
Biotechnology Letters. 19:1165-1168
Acetobacterium woodii produced catechol (up to 7.84 mM) by demethylating 2-methoxyphenol during growth in the presence or absence of fructose. The highest product concentrations were obtained when 2-methoxyphenol was the sole energy source but the hi
Publikováno v:
Biotechnology Techniques. 9:617-622
The viable count in fructose-grown cultures of Acetobacterium woodii fell to undetectable levels 186 h after the stationary phase was reached. The loss of viability was due to accumulation of undissociated acetic acid, since loss of viability could b
Publikováno v:
Biotechnology Techniques. 8:733-738
A simple procedure for screening Clostridium acetobutylicum for biotransformations to produce alcohols was developed by supplementing rich medium with glucose and butyric acid to obtain butanol-producing cultures. No special apparatus was required to
Publikováno v:
Chemical communications (Cambridge, England). (17)
Waste glycerol was converted to secondary amines in a one pot reaction, using Clostridium butyricum and catalytic hydrogen transfer-mediated amination.
Autor:
Gillian M. Stephens, Warwick B. Dunn, Alan J. Dickson, Rasmus Hansen, Arfa Maqsood, Christopher A. Sellick, Royston Goodacre
Publikováno v:
Analytical chemistry. 81(1)
Global metabolite analysis approaches, coupled with sophisticated data analysis and modeling procedures (metabolomics), permit a dynamic read-out of how cellular proteins interact with cellular and environmental conditions to determine cell status. T
Autor:
Roger M. Jarvis, Stephanie Schuler, Royston Goodacre, Gillian M. Stephens, Catherine L. Winder, David Broadhurst, Warwick B. Dunn
Publikováno v:
Analytical chemistry. 80(8)
Metabolomics and systems biology require the acquisition of reproducible, robust, reliable, and homogeneous biological data sets. Therefore, we developed and validated standard operating procedures (SOPs) for quenching and efficient extraction of met
Autor:
H. Jungnickel, John C. Vickerman, Oliver Sg, Gillian M. Stephens, Nicholas P. Lockyer, Emrys A. Jones
Publikováno v:
Analytical chemistry. 77(6)
We present a TOF-SIMS analysis of the cell surface differences between four yeast strains from two species, Candida glabrata and Saccharomyces cerevisiae (haploid strains BY4742 and BY4741 and the derived diploid BY4743). The study assesses the suita