A robust fractionation method for protein subcellular localization studies in Escherichia coli

Autor: Gilles Malherbe, David Paul Humphreys, Emma Davé

Publikováno v: BioTechniques, Vol 66, Iss 4, Pp 171-178 (2019)

Fractionation in Gram-negative bacteria is used to identify the subcellular localization of proteins, in particular the localization of exported recombinant proteins. The process of cell fractionation can be fraught with cross-contamination issues an

Externí odkaz: https://doaj.org/article/172f1e98938c49359f826783c5a43cbe

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes

Autor: Olena Pylypenko, Tobias Welz, Janine Tittel, Martin Kollmar, Florian Chardon, Gilles Malherbe, Sabine Weiss, Carina Ida Luise Michel, Annette Samol-Wolf, Andreas Till Grasskamp, Alistair Hume, Bruno Goud, Bruno Baron, Patrick England, Margaret A Titus, Petra Schwille, Thomas Weidemann, Anne Houdusse, Eugen Kerkhoff

Publikováno v: eLife, Vol 5 (2016)

There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleato

Externí odkaz: https://doaj.org/article/c2c1af684db44be18ca98b689ff18474

Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

Autor: Susanne Sievers, Daniela Zühlke, Thomas Sura, Katharina Riedel, Colin Robinson, Gilles Malherbe, Emma Dave, Rabea Schlüter, Katarzyna Magdalena Dolata, Isabel Guerrero Montero

Publikováno v: Microbial Cell Factories, Vol 18, Iss 1, Pp 1-15 (2019)
Microbial Cell Factories

Background The Twin-arginine translocation (Tat) pathway of Escherichia coli has great potential for the export of biopharmaceuticals to the periplasm due to its ability to transport folded proteins, and its proofreading mechanism that allows correct

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0905b1f3f73a1ac1933d58e44964f850
http://link.springer.com/article/10.1186/s12934-019-1071-7

Noise reduction for cochlear implant : an overview of the performances of various noise suppression techniques implemented on the digisonic cochlear implant

Autor: Gilles Malherbe, Gallego, S., olivier meste, Hervé Rix

Publikováno v: 7th International Cochlear Implant Conference
7th International Cochlear Implant Conference, 2002, Manchester, Grande Bretagne, Unknown Region
HAL

International audience

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::665790937acfb4cca6b4e5b4ea00af4d
https://hal.archives-ouvertes.fr/hal-02142728

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes

Autor: Andreas T. Grasskamp, Martin Kollmar, Janine Tittel, Margaret A. Titus, Petra Schwille, Eugen Kerkhoff, Florian Chardon, Bruno Goud, Thomas Weidemann, Gilles Malherbe, Olena Pylypenko, Annette Samol-Wolf, Bruno Baron, Patrick England, Carina Ida Luise Michel, Sabine Weiss, Alistair N. Hume, Anne Houdusse, Tobias Welz

Publikováno v: eLife
eLife, 2016, 5, pp.e17523. ⟨10.7554/eLife.17523⟩
eLife, eLife Sciences Publication, 2016, 5, pp.e17523. ⟨10.7554/eLife.17523⟩
eLife, Vol 5 (2016)
eLife, eLife Sciences Publication, 2016, 5, ⟨10.7554/eLife.17523⟩

There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleato

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b75111f3d4af95e8ee9b72d320b7cc30