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Autor:
Hisashi Kishi, Gilbert S. Greenwald
Publikováno v:
Biology of Reproduction. 61:1171-1176
The gonadotropin-primed immature rat has become the most common model for the study of follicular development and ovulation. In this study, prepubertal female rats, 23 and 24 days old, were injected s. c. with 5 IU eCG, and ovaries were collected for
Autor:
Hisashi Kishi, Gilbert S. Greenwald
Publikováno v:
Biology of Reproduction. 61:1177-1183
Prepubertal female rats were injected s.c. with 5.0 IU eCG, and ovaries were collected 24 and 48 h post-eCG, on Day 25, as well as from an untreated group also on Day 25. Large antral follicles were manually dissected, and the ovarian remnants were i
Publikováno v:
Biology of Reproduction. 58:255-260
The corpora lutea (CL) of the cyclic hamster are destroyed between Days 2 and 3 of the 4-day estrous cycle so that only one set is ever present (Day 1 = estrus, Day 4 = proestrus). The possibility that luteal cell death in the cyclic hamster is attri
Publikováno v:
Journal of Reproduction and Development. 44:305-311
A model of induced follicular atresia consists of hypophysectomizing hamsters on the morning of estrus (day 1 of cycle) followed by injecting 30 IU equine chorionic gonadotropin (eCG). On the morning of day 4 (0 h) atresia is induced by administratio
Autor:
Wei Yuan, Gilbert S. Greenwald
Publikováno v:
Experimental Biology and Medicine. 214:265-270
The purpose of this study was to determine the effects of ovine follicle-stimulating hormone (FSH), luteinizing hormone (LH); prolactin, and recombinant FSH and a protein kinase C activator (phorbol 12-myristate 13-acetate [PMA]) on progesterone prod
Autor:
Gilbert S. Greenwald, Mariko Otsuka, Gen Watanabe, Katsuhiko Arai, Hisashi Kishi, Kazuyoshi Taya
Publikováno v:
Biology of Reproduction. 56:423-429
The time course for loss of ability of Graafian follicles to secrete inhibin and estradiol was investigated during induced follicular atresia. Cyclic hamsters were hypophysectomized on Day 1 (estrus) and injected s.c. with 30 IU eCG. Thereafter, thes
Autor:
Gilbert S. Greenwald, Wei Yuan
Publikováno v:
Experimental Biology and Medicine. 209:376-381
The in vitro ability of ovine (o) follicle-stimulating hormone (FSH), (o)luteinizing hormone (LH), (o)prolactin (PRL), and recombinant human FSH (rhFSH) to stimulate progesterone (P4) synthesis by rat corpora lutea on Day 4 of pregnancy was investiga
Publikováno v:
Biology of Reproduction. 52:313-319
In vitro progesterone (P4) production by hamster luteal cells is stimulated throughout pregnancy by FSH and LH. Prolactin (PRL) by itself, however, increases P4 synthesis only on Day 12; on Day 4, FSH+LH+PRL induces optimal P4 secretion [Biol Reprod
Autor:
Gilbert S. Greenwald, Wei Yuan
Publikováno v:
Biology of Reproduction. 51:472-479
We have recently shown that FSH, LH, and prolactin (PRL)--alone or combined--act as luteotropins when incubated with luteal cells from pregnant hamsters (Yuan and Greenwald, Biol Reprod 1994; 51:43-49). The purpose of the present study was to determi