Zobrazeno 1 - 10 of 13 pro vyhledávání: '"Gijsbert J Jansen"'
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Akademický článek
Direct detection of SARS-CoV-2 antisense and sense genomic RNA in human saliva by semi-autonomous fluorescence in situ hybridization: A proxy for contagiousness?
Autor: Gijsbert J Jansen, Marit Wiersma, Willem J B van Wamel, Inge D Wijnberg
Publikováno v: PLoS ONE, Vol 16, Iss 8, p e0256378 (2021)
Saliva is a matrix which may act as a vector for pathogen transmission and may serve as a possible proxy for SARS-CoV-2 contagiousness. Therefore, the possibility of detection of intracellular SARS-CoV-2 in saliva by means of fluorescence in situ hyb
Externí odkaz: https://doaj.org/article/d119fa847a0f4c4483adcfa1d84f9fc0
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2
Evaluation of a fluorescence in situ hybridization (FISH)-based method for detection of SARS-CoV-2 in saliva
Autor: Gerrit G. Tamminga, Gijsbert J. Jansen, Marit Wiersma
Publikováno v: PloS one. 17(11)
The use of a non-invasive fluorescence in situ hybridization (FISH)-based method on saliva for the detection of SARS-CoV-2 is evaluated in a proof-of-concept study and thereafter utilized in an outpatient setting with the Biotrack-MED® analyzer. For
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0fdeaed82d451448438bf317abe6665
https://pubmed.ncbi.nlm.nih.gov/36346813
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3
Nine-month presence of SARS-CoV-2 in saliva: a case report
Autor: Gijsbert J. Jansen, Marit Wiersma
SARS-CoV-2 is a novel coronavirus that mainly affects the upper airways. Approximately one third of all detected cases is asymptomatic. We report an asymptomatic individual who tested positive for SARS-CoV-2 over a period of nine months. Of this indi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::84e8d4918bf576d6697fe5007884e53a
https://doi.org/10.1101/2021.10.08.21262462
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4
Development and validation of an alternative parameter for quantification of signals emitted by fluorescently labelled bacteria in microscopic images
Autor: Gijsbert J. Jansen, Gerrit G. Tamminga, Gert-Jan Euverink, Astrid H. Paulitsch-Fuchs
Publikováno v: Journal of microbiological methods, 166:105717. ELSEVIER SCIENCE BV
In this study, an alternative parameter for quantifying the signals of fluorescently labelled bacteria (e.g. propidium iodide, Cyanine 3, etc.) in microscopic images was investigated. Three common parameters (mean grey value (MGV), mean grey value wh
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c8bf293f0916d8d789607885d8b6282
https://research.rug.nl/en/publications/1415839f-fbda-4f47-ad9c-dd2e09c9adf2
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5
Different binarization processes validated against manual counts of fluorescent bacterial cells
Autor: Gijsbert J. Jansen, Astrid H. Paulitsch-Fuchs, Gerrit G. Tamminga, Gert-Jan Euverink
Publikováno v: Journal of microbiological methods, 128, 118-124. ELSEVIER SCIENCE BV
State of the art software methods (such as fixed value approaches or statistical approaches) to create a binary image of fluorescent bacterial cells are not as accurate and precise as they should be for counting bacteria and measuring their area. To
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2559cd2e1636dbc5ec1726be832b4106
https://doi.org/10.1016/j.mimet.2016.07.003
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8
Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes
Autor: Hermie J. M. Harmsen, Gijsbert J. Jansen, Gjalt W. Welling, Gerwin C. Raangs, Alison H. Franks, Frits Schut
Publikováno v: Applied and environmental microbiology, 64(9), 3336-3345. AMER SOC MICROBIOLOGY
Six 16S rRNA-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples. A set of two probes was specific for species of the Bacteroides fragilis group and the specie
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::040c603e2fb5cdb6f59c3f5267f5b837
https://doi.org/10.1128/aem.64.9.3336-3345.1998
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9
16S ribosomal RNA-targeted oligonucleotide probes for monitoring of intestinal tract bacteria
Autor: Gerwin C. Raangs, P Elfferich, Gjalt W. Welling, A. C. M. Wildeboer-Veloo, Gijsbert J. Jansen, John E. Degener
Publikováno v: SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 32, 17-19. Taylor & Francis Group
Background. The composition of a sample of faecal bacteria can be determined by culturing different dilutions on specific media. However, not all bacteria can be cultured and media are not always specific. With a culture-independent approach a more a
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a388161f7cbe59b1640b2f7e8b67eeec
https://hdl.handle.net/11370/cea78cb1-740f-47ae-9e49-af66e3e7450a
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10
Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples
Autor: Frits Schut, Gerwin C. Raangs, Gjalt W. Welling, Gijsbert J. Jansen, Ger R. Kamphuis, Michael H. F. Wilkinson, Petra S. Langendijk
Publikováno v: Applied and Environmental Microbiology, 61, 3069-3075
Applied and Environmental Microbiology, 61, 8, pp. 3069-3075
Applied Environmental Microbiology, 61(8). AMER SOC MICROBIOLOGY
Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applic
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b157b9ca89338b7ab7d0a5eb9e5a1cc2
http://hdl.handle.net/2066/20973
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